1. Spiky titanium dioxide nanoparticles-loaded Plantaginis Semen polysaccharide as an adjuvant to enhance immune responses.
- Author
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Ren, Zhe, Yu, Ruihong, Meng, Zhen, Sun, Mengke, Huang, Yongyuan, Xu, Ting, Guo, Qiong, and Qin, Tao
- Subjects
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IMMUNOLOGICAL adjuvants , *SEMEN , *TITANIUM dioxide , *IMMUNE response , *T cells , *POLYSACCHARIDES - Abstract
The purpose of this study was to prepare spiky titanium dioxide nanoparticles-loaded Plantaginis Semen polysaccharide (SN-TiO 2 -PSP), and the structural characterization and immune response of infectious laryngotracheitis (ILT) vaccine in Hetian chickens were investigated. The structural characterization of SN-TiO 2 -PSP was analyzed by FT-IR, TEM, and TGA analysis. And the immune organs indexes, lymphocytes proliferation, specific antibody levels, and ratios of CD4+ and CD8+ T lymphocytes were studied. Structural characterization results showed that SN-TiO 2 -PSP has a typical polysaccharide absorption peak and good stability. The SN-TiO 2 -PSP's shape was similar to sea urchin, and its zeta potential and particle size were 27.56 mV and 976.11 nm, respectively. In vivo results showed that SN-TiO 2 -PSP could enhance the proliferation of peripheral lymphocytes, specific antibody levels, CD4+ and CD8+ T lymphocytes ratios, IL-4 and INF-γ levels in Hetian chickens vaccinated with ILT vaccine on D 7 , D 14 , D 21 , and D 28. In addition, SN-TiO 2 -PSP not only enhanced the indexes of immune organs but also promoted the development of immune organs. Therefore, SN-TiO 2 -PSP has immune adjuvant activity and may become a new potential immune adjuvant. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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