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Due to its low molecular weight and abundant functional groups, water-soluble lignin (WSL) is considered as a more potent antioxidant than traditional industrial lignin in biofields. However, few studies have been conducted to evaluate its intracellular and endogenous reactive oxygen species (ROS)-scavenging ability, especially for the intervention of ROS-related disease in vivo. In this work, WSL in bamboo autohydrolysate (WSL-BM) and wheat stalk autohydrolysate (WSL-WS) were isolated and characterized to comparably analyze their bioactivities. The composition analysis and NMR characterization showed that both WSL-BM and WSL-WS contained relatively similar components and substructures, but WSL-BM contained higher contents of phenolic OH groups. Both WSL samples exhibited excellent biocompatibility with the concentration below 50 μg/mL, while WSL-BM exhibited superior ROS-scavenging ability and ROS-related ulcerative colitis treatment potential at same concentration. In addition, WSL-BM also showed better performance in ameliorating inflammation and oxidative stress in RAW 264.7 cells and colitis mice by activating Nrf2 and suppressing NFκB signaling, resulting in an overall improvement in both macroscopic and histological parameters. Overall, these results implied that WSL from gramineous biomass can be used as a novel anti-inflammatory and antioxidative agent in the biomedical field. This work shows the application of water-soluble lignin (WSL) extracted from bamboo (BM) and wheat stalk (WS) as as a bioactivator (anti-inflammatory and antioxidative) for ulcerative colitis. [Display omitted] [ABSTRACT FROM AUTHOR]

New antibacterial agents are needed to overcome the challenges of microbial food contamination. In this study, we investigated the potential of Elaeagnus mollis polysaccharide (EMP) to modify black phosphorus (BP) for use as a bactericide for foodborne pathogenic bacteria. The resulting compound (EMP-BP) displayed enhanced stability and activity compared with BP. EMP-BP exhibited an increased antibacterial activity (bactericidal efficiency of 99.999 % after 60 min of light exposure) compared to EMP and BP. Further studies revealed that photocatalytically generated reactive oxygen species (ROS) and active polysaccharides acted collectively on the cell membrane, leading to cell deformation and death. Furthermore, EMP-BP inhibited biofilm formation and reduced expression of virulence factors of Staphylococcus aureus , and material hemolysis and cytotoxicity tests prove that the material had good biocompatibility. In addition, bacteria treated with EMP-BP remained highly sensitive to antibiotics and did not develop significant resistance. In summary, we report an environmentally friendly method for controlling pathogenic foodborne bacteria that is efficient and apparently safe. [Display omitted] • The novel EMP-BP nanomaterial composite can be cheaply and easily fabricated. • Designing EMP over BP for enhancing material stability and antibacterial activity. • EMP-BP inhibits bacteria by photogenerated ROS and natural active ingredients. [ABSTRACT FROM AUTHOR]

New antibacterial agents are needed to overcome the challenges of microbial food contamination. In this study, we investigated the potential of Elaeagnus mollis polysaccharide (EMP) to modify black phosphorus (BP) for use as a bactericide for foodborne pathogenic bacteria. The resulting compound (EMP-BP) displayed enhanced stability and activity compared with BP. EMP-BP exhibited an increased antibacterial activity (bactericidal efficiency of 99.999 % after 60 min of light exposure) compared to EMP and BP. Further studies revealed that photocatalytically generated reactive oxygen species (ROS) and active polysaccharides acted collectively on the cell membrane, leading to cell deformation and death. Furthermore, EMP-BP inhibited biofilm formation and reduced expression of virulence factors of Staphylococcus aureus , and material hemolysis and cytotoxicity tests prove that the material had good biocompatibility. In addition, bacteria treated with EMP-BP remained highly sensitive to antibiotics and did not develop significant resistance. In summary, we report an environmentally friendly method for controlling pathogenic foodborne bacteria that is efficient and apparently safe. [Display omitted] • The novel EMP-BP nanomaterial composite can be cheaply and easily fabricated. • Designing EMP over BP for enhancing material stability and antibacterial activity. • EMP-BP inhibits bacteria by photogenerated ROS and natural active ingredients. [ABSTRACT FROM AUTHOR]