Search

In recent years, natural resources have proven to be tremendous sources of glycoproteins. As biological macromolecules, glycoproteins are essential to the growth and development of organisms, and have attracted increasing attention around the world. This review summarized and discussed the development of glycoproteins from natural resources, including isolation methods, purification processes, structural features and biological activities. Generally, the vast majority of glycoproteins can be isolated by hot water extraction followed by purification through gel filtration chromatography. Combined with component analysis, the physicochemical properties of glycoproteins are studied by using several spectroscopic techniques such as ultraviolet-visible (UV–visible), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR). Moreover, natural glycoproteins possess various remarkable biological activities, including anti-tumor, anti-oxidant, anti-coagulant and anti-microbial activities. The content of this review will provide a theoretical basis for the research on related glycoproteins and give a perspective on the use of these medical resources. [ABSTRACT FROM AUTHOR]

Nanoscale metal–organic frameworks (NMOFs) have attracted considerable attention for controlled drug delivery. However, the interaction between nanoparticles and the biological macromolecules of physiological system must be valued because the formed protein corona will endow NMOFs with new biorecognition properties. In this study, we carried out detailed protein adsorption studies in vitro and cell uptake tests of HeLa cells for nanospherical Uio66 and nanooctahedral Uio67. Uio67 with higher binding constants to human serum albumin needed to combine more protein molecules to achieve colloidal stability state than that needed by Uio66, and this phenomenon led Uio67 to aggregate under the same incubation condition due to the formation of a single-layer protein. Uio67 also induced an evident conformation change in protein to stabilize the combination. In particular, the cell uptake efficiencies of the two systems showed a significant thickness dependence on the protein corona. When samples incubated in 10% fetal bovine serum (FBS), the intracellular rate was the highest for both systems, but the rate was not proportional to the FBS concentration. Results of this work are important to the development of the considerable potential NMOFs-based medicals and also provide additional insight into protein corona. Unlabelled Image • Distinct morphologies impacted on surface chemistry and cell uptake differently. • Cell uptake efficiencies showed thickness dependence on the protein corona. • Uio67 needed to combine more protein to achieve the colloidal stability state • HSA experienced different degrees conformation change to stabilize the combination. [ABSTRACT FROM AUTHOR]