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Wounds are one of the most critical clinical issues in plastic surgery repair and restoration. Conventional wound dressing materials cannot absorb enough wound exudates and shield the site from microbial infection. Also, despite their healing prowess, bioactive molecules from medicinal plants are less bioavailable at the wound sites. This study developed a 3D-printed hydrogel of sodium alginate and gelatin loaded with freeze-dried Aloe barbadensis extract for enhanced wound healing. The hydrogel was hydrophilic and showed an average pore size of 163.66 ± 14.45 μm, moderate swellability, and ideal mechanical properties with tensile strength(σ) of 16.39 ± 0.98 MPa, and Young's modulus of 17.43 ± 1.41 MPa. They showed potential antibacterial activity against Staphylococcus aureus (87.7 ± 4 % inhibition) and Pseudomonas aeruginosa (84.4 ± 6 % inhibition). These hydrogels were hemocompatible, biocompatible, and biodegradable. Cell cytotoxicity assay and scratch assay showed effective Normal Human Dermal Fibroblast cells (NHDF) viability, proliferation, and migration on the hydrogel. In vivo studies of the 3D-printed hydrogel demonstrated significantly improved wound closure, reduced wound contraction, enhanced epithelial regeneration with minimal inflammation, and decreased scar formation after 14 days of treatment. Therefore, this 3D-printed hydrogel can be promising for wound healing with scar reduction. [Display omitted] • Aloe barbadensis loaded sodium alginate-gelatin hydrogel showed shear thinning and non-newtonian behavior • 3D Printed SA-G-ALB hydrogel scaffold was biodegradable, non-toxic, biocompatible and mechanically sturdy material • SA-G-ALB scaffold improved the bioavailability of Aloe barbadensis active molecules for wound healing • This SA-G-ALB scaffold showed full-thickness wound closure with significantly lesser contraction and reduced scar formation [ABSTRACT FROM AUTHOR]

Gastro retentive drug delivery systems (GRDDS) have gained immense popularity as they reduce dosing frequency, improve bioavailability, and enhance patient compliance. Herein, a plant-based, controlled swelling, and pH-sensitive GRDDS based on Aloe vera hydrogel and cellulose was developed for the sustained release of levofloxacin (LEVO). The properties of five various floating tablet formulations including dynamic swelling, pH-responsiveness, hardness, friability, drug release, and buoyant time were evaluated. The optimized formulation (FF) was characterized using FTIR and SEM, and the surface morphology exhibited a porous texture with microchannels that facilitated tablet swelling and prolonged release of LEVO. The formulation FF remained buoyant (> 12 h) in the simulated gastric fluid with a buoyancy time of 303 s. A pH-dependent swelling behavior of the formulation FF was revealed with the highest swelling (7.1 g/g) in water, followed by buffers of pH 6.8 (5.4 g/g), 4.5 (3.8 g/g), and 1.2 (2 g/g). The controlled release of LEVO was demonstrated for >12 h following the Hixson-Crowell model and non-Fickian diffusion. Pharmacokinetic parameters of LEVO were determined using in vivo studies. The non-toxic nature of the formulation under study was demonstrated. The results render this approach promising in reducing the dosing frequency, suggesting its potential for clinical applications. [Display omitted] • Natural hydrogels based on Aloe vera and cellulose were developed as levofloxacin drug carrier. • Optimized tablet formulation revealed efficient sustained drug release. • Developed formulation showed longer floating time than market tablet. • pH-sensitive swelling behavior was demonstrated. • In vivo and in vitro studies unraveled the pharmacokinetics of drug release. [ABSTRACT FROM AUTHOR]

Dental pulp regeneration exploits tissue engineering concepts using stem cells/scaffolds/growth-factors. Extracted collagen is commonly used as a biomaterial-scaffold due to its biocompatibility/biodegradability and mimics the natural extracellular matrix. Adding biomolecules into a collagen-scaffold enhanced pulp regeneration. Acemannan, β-(1-4)-acetylated-polymannose, is a polysaccharide extracted from aloe vera. Acemannan is a regenerative biomaterial. Therefore, acemannan could be a biomolecule in a collagen-scaffold. Here, acemannan and native collagen were obtained and characterized. The AceCol-scaffold's physical properties were investigated using FTIR, SEM, contact angle, swelling, pore size, porosity, compressive modulus, and degradation assays. The AceCol-scaffold's biocompatibility, growth factor secretion, osteogenic protein expression, and calcification were evaluated in vitro. The AceCol-scaffolds demonstrated higher hydrophilicity, swelling, porosity, and larger pore size than the collagen scaffolds (p < 0.05). Better cell-cell and cell-scaffold adhesion, and dentin extracellular matrix protein (BSP/OPN/DSPP) expression were observed in the AceCol-scaffold, however, DSPP expression was not detected in the collagen group. Significantly increased cellular proliferation, VEGF and BMP2 expression, and mineralization were detected in the AceCol-scaffold compared with the collagen-scaffold (p < 0.05). Computer simulation revealed that acemannan's 3D structure changes to bind with collagen. In conclusion, the AceCol-scaffold synergistically provides better physical and biological properties than collagen. The AceCol-scaffold is a promising material for tissue regeneration. [ABSTRACT FROM AUTHOR]

Plant-based polymers hold promising prospects thanks to their bioactivity, diversity and versatility but they are currently overshadowed by synthetic and animal-derived materials, especially in biomedical applications. In this study, we developed an entirely plant-based hydrogel with improved mechanical performance based on TEMPO-oxidized cellulose nanofibrils (TCNFs) and the polysaccharide fraction (AVPF) extracted from Aloe vera L. (Aloe barbadensis Miller). The hydrogel blends exhibited excellent viscoelastic properties, minimal shrinkage and a significant increase in compressive modulus (ranging from 2.7 to 13.2 kPa versus 0.8 kPa in single component hydrogels), suggesting a synergistic effect. In-depth analysis of interaction and morphology of the hydrogels by QCM-D, AFM and SEM imaging showed that the observed synergy was the result of the complementary action between the two components and a uniform spatial distribution of the two networks. TCNFs built the rigid skeleton for the hydrogels, while AVPF physically adsorbed on TCNFs, forming a flexible matrix, allowing for better load transfer and dissipation in both static and dynamic loading, leading to a remarkable increase in moduli that surpassed the mere sum of the two individual components. In addition, the obtained hydrogels also showed little to no perceptible shrinkage after drying, unlike the single-component hydrogels made from the initial materials. These hydrogels offer a sustainable and ethical alternative to animal-derived materials, with great potential in biomedical fields., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.)

Jamun (Syzygium cumini L. Skeels), a less recognized, underutilized, and highly perishable fruit is a delicacy of tropical regions. Soft pulp and thin exocarp make these small purple berries susceptible to mechanical injury and several postharvest diseases. Therefore, this study aimed to evaluate the potential of Aloe vera gel (AG), hydroxypropyl methylcellulose (HPMC) and Adhatoda vasica Nees. leaves extract (AVLE) coatings for enhancing postharvest shelf-life and quality of jamun fruits stored at 60–65%RH and 9 ± 2 °C. Antifungal, physicochemical, biochemical and cell wall softening enzyme assays were conducted to assess the quality changes in jamun during storage. Our findings reveal that incorporating AG and AVLE in the edible coatings reduces the disease severity of the coated fruits compared to control fruits. Additionally, AG + HPMC + AVLE (2 %) and (4 %) preserved jamun for 22 and 24 days, respectively, with better nutritional quality against control (15 days). The coated fruits, especially, AG + HPMC + AVLE (4 %) showed minimal weight loss 32.92 % with no fungal incidents by the end of storage. Moreover, AG + HPMC + AVLE (4 %) maintained pH (3.68), TA (0.85 %), and TSS (9.67°Brix) than control fruits. Also, AG + HPMC + AVLE (2 %) effectively delayed fruit quality deterioration by suppressing cell wall-softening enzyme activity, and thus slowing the increase in reducing and non-reducing sugars. This study highlights a novel edible coating of Aloe vera gel and A. vasica leaves extract with excellent potential to maintain the quality and extend the shelf-life of jamun fruits after harvest. • HPMC incorporated Aloe vera gel (AG) edible coating with improved physical and barrier characteristics. • The novel plant based antimicrobial Adhatoda vasica Nees. (leaves extract) was incorporated in edible coating. • AG + HPMC + AVLE coating Extended shelf life to maximum 24 days of the jamun fruit. • Physical and chemical characteristics of jamun fruits were maintained during their postharvest storage. • Edible coating treatments delayed wall softening lead to lesser accumulation of sugars and soluble solids in the fruit. [ABSTRACT FROM AUTHOR]

Medical wound dressings are effective in protecting wounds, maintaining moisture, creating an optimal healing environment and accelerating wound healing. However, their deficiencies in mechanical properties, adhesion and prevention of adhesion to the wound bed have been identified as limiting factors for their therapeutic efficacy in wound healing. To address these issues, we prepared glycosilicone gel dressings consisting of hydrophobic polysiloxanes and highly hydrophilic polysaccharides via ester exchange and silicone hydrogen addition reactions. Silicone gel dressings exhibit skin-like "respiratory" properties, with good permeability to O 2 and CO 2. Additionally, elongation and other important parameters are similar to those of the skin, which provides a foundation for the application of silicone gels in the field of wound dressings. The introduction of Aloe vera polysaccharide (AP) results in the glycosilicone gel exhibiting certain mechanical properties, including a tensile strength of 0.35 MPa and an adhesion force of 10 N/m. Furthermore, a mouse model of total skin defect demonstrated that the wound healing rate of the mice on the 12th day was 98 %, which effectively promotes wound healing. Consequently, the glycosilicone gel is anticipated to be an optimal wound dressing. [ABSTRACT FROM AUTHOR]

Macromolecule hydrogels made from natural extracts have received much attention because of their favorable biocompatibility and wound healing properties. However, their clinical applications are limited by their insufficient mechanical strength and low adhesion properties. To overcome these limitations, we developed a novel injectable Aloe vera hydrogel (PDMA-GelMA@AV). By integrating gelatin methacrylate (GelMA) and polydopamine methacrylamide (PDMA), we significantly improved the mechanical and adhesion properties of the hydrogel. The PDMA-GelMA@AV hydrogel degraded in a simulated wound environment, which was synchronized with the sustained release of the bioactive components of A. vera. In vitro and in vivo analyses revealed that this hydrogel has good biocompatibility. In vitro studies also revealed that the sustained release of the active ingredients of A. vera promoted fibroblast proliferation and migration and increased the expression of key proteins and mRNAs required for wound healing. In addition, it modulated LPS-stimulated macrophages and decreased the expression of TNF-α, IL-1β and iNOS while increasing the expression of TGF-β and ARG. In vivo experiments further confirmed the efficacy of hydrogels in wound healing applications. These findings offer a novel perspective on the application of natural macromolecules as hydrogel-based delivery vehicles in wound care. [ABSTRACT FROM AUTHOR]

Hydrogel wound dressings provide a moist environment, which promotes the formation of granulation tissue and epithelium in the wound area, accelerating the wound healing process. There have been numerous approaches to skin wound management and treatment, but the limitations of current methods highlight the need for more effective alternatives. A Chitosan polyherbal hydrogel integrated with AgNPs was synthesized to assess its wound-healing potential both in vitro and in vivo. The AgNPs were synthesized using Calotropis procera leaf extract and characterized via X-ray diffraction analysis (XRD), Scanning electron microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FT-IR). In swelling kinetic analysis, the hydrogel's weight reached its maximum at 8 h of incubation and began to decrease from 12 h up to 72 h (49 % ± 6.04). The hydrogel formulation demonstrated strong antimicrobial potential against E. coli and S. aureus with an inhibition zone of 18 mm and 25 mm, respectively. Furthermore, in mice studies, the formulation exhibited significant wound size reduction within 12 days, supported by histopathology analysis revealing higher angiogenic potential compared to commercial hydrogels. The concentrations of IL-6 and TNF-α in CS-polyherbal/AgNPs hydrogel were 500 pg/ml and 125 pg/ml, respectively. Additionally, a network pharmacology approach identified 11 chemical constituents in Aloe vera, Azadirachta indica , and Alternanthera brasiliana extracts, along with 326 potential targets, suggesting the superior wound healing properties of this formulation compared to commercially available hydrogels. • A novel hydrogel was synthesized by integrating chitosan with AgNPs using Calotropis procera leaf extract. • XRD, SEM, and FT-IR were used to examine and verify the structure and synthesis of AgNPs-incorporated hydrogel. • In-vitro studies show antibacterial action against E. coli, S. aureus , while in-vivo mice tests reveal faster wound healing than market hydrogels. • A network pharmacology method was used to discover active components, targets, and signaling pathways for wound healing. [ABSTRACT FROM AUTHOR]

This study aimed to enhance the properties of polyvinylpyrrolidone (PVP) for use as biocompatible facial masks. To achieve this, nanofibers were developed by blending PVP with cellulose nanofibers (CNFs) and Aloe vera (AV) powder using electrospinning. The results showed that incorporating CNFs and AV into the PVP matrix led to the formation of smooth and uniform nanofibers. In particular, adding 3–6 wt% AV powder in PVP/CNF composites improved fiber diameter distribution and uniformity compared to pure PVP. The PVP/CNF/AV nanofibers exhibited desirable properties for facial mask applications. They displayed 86–93 % porosity, which allowed for efficient moisture absorption capacity of up to 1829 %, and excellent water vapor permeability rate of 3.92 g/m2h. The mechanical properties of the electrospun nanofiber composites were evaluated through tensile testing. The results showed that Young's modulus values decreased progressively with the addition of CNFs and AV powder to the PVP polymer matrix, indicating a plasticizing effect that enhances flexibility. The fracture strain remained similar across all composites, suggesting that CNFs and AV did not significantly weaken the PVP matrix. The tensile strength initially increased with CNF addition but decreased with incremental AV loading. Biocompatibility studies revealed that all nanofibers exhibited excellent fibroblast viability, surpassing 98 %. This indicates that incorporating CNFs and AV did not compromise cell viability, further highlighting the suitability of the PVP/CNF/AV composites for facial mask applications. [ABSTRACT FROM AUTHOR]

Despite the outstanding characteristics of alginate, it suffers from bad UV-barrier, water barrier, and antimicrobial properties limiting its wide usage in food packaging. For this concern, Box-Behnken design (BBD) was applied to prepare an alginate-based edible coating of the best optimized UV-shielding properties upon the incorporation of both Aloe vera (AV) and zinc oxide nanoparticles (ZnO-NPs). The optimized minimum UV-transmittance was 4.96% when the optimized compositions of alginate (1.05 g), AV (2.95 g), and ZnO-NPs (4.93 wt%) were used. FTIR was used to verify the successful edible coating preparation while the wide-angle X-ray diffraction (XRD) was used to identify the interactions between the film's components. The incorporation of both AV and ZnO-NPs significantly improved alginate's thermal, water vapor permeability (WVP), mechanical and antimicrobial properties. In addition, the films incorporated with both AV and ZnO-NPs exhibited excellent UV-barrier properties compared with neat alginate. The optimized alginate film incorporated with both ZnO-NPs and AV significantly extended the shelf-life of tomato fruits up to 16 days without any defects. Due to the outstanding physical, UV-shielding, and antimicrobial properties of the optimized alginate/AV/ZnO-NPs film, it could be used potentially in food packaging industries. • Aloe vera AV and ZnO nanoparticles ZnO-NPs were incorporated into alginate coating. • UV shielding properties were optimized by using Box-Behnken design. • AV and ZnO-NPs incorporation improved the alginate packaging efficiency. • The prepared coating inhibited the spoilage of tomatoes. • The optimized coating might be used potentially in food packaging applications. [ABSTRACT FROM AUTHOR]

Maintaining a moist and sterile environment is conducive to accelerating wound healing. To develop a natural wound dressing with good water retention capacity and antibacterial activity, we prepared a novel natural multifunctional hydrogel for infected wound healing, which combines the advantages of Aloe polysaccharide (AP) and honey. AP was extracted from Aloe barbadensis , and its structure was characterized by fourier transform infra-red (FT-IR) spectoscopy and nuclear magnetic resonance (NMR) spectroscopy. AP is an acetylated mannan composed of (1 → 4)β-Man p , which is acetylated at C-2, C-3 and C-6 positions. AP/Honey@PVA hydrogel was prepared by cross-linking AP, honey, PVA with borax, which has good mechanical strength and excellent biocompatibility for blood cells, NIH-3T3 cells and L929 cells. The hydrogels showed significant inhibitory effect on Staphylococcus aureus , Escherichia coli and Candida albicans , as well as accelerated the healing of infected full-thickness wound. This study reveals the structure of AP and proves that AP and honey composite hydrogel has potential application prospect in the therapy of infected wounds. • An acetylated (1 → 4)β-mannan (AP) was extracted from Aloe barbadensis. • AP/honey/PVA composite hydrogel with broad antibacterial activity was developed. • The composite hydrogel accelerated the healing of infected wounds. [ABSTRACT FROM AUTHOR]

Immense socio-economic burden of chronic wound demands effective, low-cost strategies for wound care. Herein, we have developed a chemical crosslinker-free phyto-hydrogel by encapsulating phytochemicals of Aloe vera mucilage extract (AVM) in the self-assembled polymeric chains of two different silk fibroin (SF) proteins (from Bombyx mori and Antheraea assamensis). Additionally, polyvinylpyrrolidone (PVP) has been used as a stabilizer that also contributed to the mucoadhesive property of the composite (SAP; made of SF, AVM, and PVP) hydrogel. The physicochemical properties of the hydrogel were evaluated and compared with SF hydrogel containing only SF proteins without any additives. The biocompatibility assessment of the hydrogel under in vitro conditions has shown improved cellular proliferative and migratory responses, suggesting faster tissue repairability of the hydrogel. A detailed in vivo comparative study with a commercially available DuoDERM® gel revealed that SAP hydrogel not only promoted wound closure but also showed better deposition and remodeling of the extracellular matrix. Moreover, the hydrogel also demonstrated its ability to downregulate pro-inflammatory markers (IL-1β, TNF-α) and upregulation of anti-inflammatory markers (IL-10, TGF-β) at the early stage of healing. Therefore, the bioactive proteins-carbohydrates composite efficiently accelerates skin regeneration and possesses great translational potential to offer a low-cost alternative wound care therapeutic. [ABSTRACT FROM AUTHOR]

The applications of edible coatings stemmed exclusively from alginate in food packaging are restricted due to their inherent deficient antimicrobial, barrier, and UV-barrier properties. In this work, we aimed to design smart alginate-based coatings for active food packaging through the addition of both aloe vera (AV) and garlic oil (GO). The interactions between the film components were verified by FTIR and XRD. Thermal and mechanical properties were improved by the presence of AV and GO. The presence of AV and GO did not significantly influence the transparency of alginate films. The films exhibited a significant UV-shielding to all UV regions. Water vapor permeability was significantly (p < 0.05) reduced either through the incorporation of AV or GO. The antimicrobial properties of the prepared films were considerably improved by the presence of AV and GO. The shelf-life of tomatoes (Solanum lycopersicum L.) was extended when coated with the alginate film incorporated with AV and GO. Owing to the outstanding UV-shielding, mechanical, thermal, and antimicrobial properties, the alginate/AV/GO active coatings could potentially be implemented in the food packaging industry. • Aloe vera (AV) and garlic oil (GO) were incorporated into alginate. • UV-shielding properties were significantly improved compared with neat alginate. • GO and AV significantly improved thermal, mechanical and antimicrobial properties. • The prepared active edible films extended the shelf-life of tomatoes. • The prepared active edible films could be used potentially in food packaging. [ABSTRACT FROM AUTHOR]

Thermocompression was employed to prepare citric acid-crosslinked chitosan films with Aloe vera (AV) as bioactive compound. Films were easy to handle and mechanical properties did not change with the addition of AV up to 10 wt%, although both TS and EAB decreased for the films with 15 wt% AV, indicating that high AV contents would hinder intermolecular interactions among the formulation components. Maillard reaction occurred between chitosan and citric acid at the processing temperature used (115 °C), while physical interactions took place with AV, as shown by FTIR analysis. All films were insoluble but displayed hydration and limited swelling due to both physical and chemical interactions promoted by AV and citric acid, respectively. A slow AV release, governed by a Fickian diffusion controlled mechanism, and an increase of surface hydrophilicity, which favors cell adhesion, were observed. [ABSTRACT FROM AUTHOR]

This work aims to prepare multifunctional edible coating based on alginate and Aloe vera (AV) optimized to minimum water vapor permeability (WVP) using Box-Behnken design. Titanium oxide nanoparticles (n TiO 2) were incorporated into the optimized film at different contents. FTIR confirmed the structures, while XRD confirmed n TiO 2 incorporation with a size ranging from 20.38 to 28.81 nm. The UV-shielding was enhanced either in the presence of AV or n TiO 2. Thermal stability by TGA was improved upon increasing the n TiO 2 content. WVP was significantly decreased from 21.53 g mm/m2 day kPa for calcium alginate to 7.18 g mm/m2 day kPa in the presence of AV and n TiO 2 (5 wt%). Color measurements showed non-significant changes in the film's transparency after AV and n TiO 2 incorporation. Mechanical and antimicrobial properties were improved in the presence of n TiO 2. Shelf-life studies on tomatoes showed significant resistance to mass loss and spoilage when coated with (alginate/AV) film containing 5 wt% of n TiO 2. • Alginate and Aloe vera were optimized using Box-Behnken design to a minimum WVP. • n TiO 2 was added to the optimized system at 1, 3 and 5% contents. • n TiO 2 improved WVP, thermal, mechanical, UV-shielding and antimicrobial properties. • n TiO 2 did not affect the film's transparency. • n TiO 2 nanocomposite extended the shelf-life of tomatoes. [ABSTRACT FROM AUTHOR]

Mannanases are ubiquitous enzymes and are being explored for diverse industrial applications. In this study, a novel bacterial strain Microbacterium sp. CIAB417 was identified and characterized for extracellular production of mannanase. Microbacterium sp. CIAB417 was found to produce maximum mannanase after 36 h of incubation at 37 °C. Mannanase produced by the isolate was observed for maximum activity at optimum pH of 6 and optimum temperature of 50 °C. Crude mannanase was found to be capable of producing mannooligosachharides (MOS) by hydrolyzing hemicellulose from locust bean gum and Aloe vera. The produced MOS was characterized and found to be mixture of mannobiose to mannohexose units. Mannanase was also explored for decolorization of dyes. Bromophenol blue and coomassie blue R-250 were observed to be decolorized to the extent of 45.40 and 42.75%, respectively. Hence, the identified bacterial strain producing mannanase could be of great significance for applications in food and textile industry. [ABSTRACT FROM AUTHOR]

At present, more and more attention has been paid to the development of active wound dressings. Chitosan, a kind of carbohydrate polymer with good biocompatibility, is widely used in the field of wound dressings. In this study, a slopeing free surface electrospinning (SFSE) device was presented to prepare large quantities of polycaprolactone/chitosan/aloe vera (PCL/CS/AV) nanofiber membranes (NFMs) for antibacterial wound dressing. And the morphologies of PCL/CS/AV NFMs with varying weight ratios of PCL:CS:AV were studied using SEM, and the optimal weight ratio of 5:3:2 was determined for better wound dressings. Then the structure, wetting property and yield of the PCL/CS/AV NFMs with the optimal weight ratio were investigated, and the effects of the addition of AV on the antibacterial performance and the biocompatibility of NFMs was studied. In addition, the preparation mechanism of SFSE was researched by simulating the electric field distribution using Maxwell 3D due to the important role of the electric field in the SFSE process. The simulation analyses of electric fields agreed with the experimental data. The results illustrated SFSE could prepare high quality PCL/CS/AV NFMs in batches, and its yield of PCL/CS/AV NFMs was 10 times more than the single-needle ES, and the fabricated NFMs showed excellent antibacterial performance and biocompatibility, which made them suitable for wound dressings. [ABSTRACT FROM AUTHOR]

It is of great clinical significance to design wound dressing materials with combined excellent wound healing properties and superior capability to suppress hypertrophic scar formation. This study aimed to examine if and how the cationicity of chitosan would affect the hypertrophic scar-related outcomes, through preparing carboxymethyl chitosan hydrogels with different genipin concentrations (2.5%, 5%, 10% and 15%, respectively). An optimum window of chitosan cationicity (5% in our case) demonstrated potential to mitigate hypertrophic scar in wound healing by suppressing the expression of a-smooth muscle actin (a-SMA) and promoting secretion of type I matrix metalloproteinases (MMP-1). In vivo, the CMCS-5% hydrogel again showed smaller, thinner and smoother wound appearance. Moreover, the CMCS-5% sample with additional incorporation of 2% (V/V) Aloe vera gel exhibited further improved performance in scar inhibition. Overall, such findings might have important implications in chitosan-based wound dressing design for high-quality wound repair and effective scar inhibition. • Chitosan cationicity was modulated with different concentrations of genipin. • Cationicity of chitosan hydrogels affected expression levels of a-SMA and MMP-1. • Chitosan-5% hydrogel showed the best prevention effect for hypertrophic scar. • Co-treatment with CMCS-5% and Aloe vera gel further improved wound appearance. [ABSTRACT FROM AUTHOR]

Recently, the use of nanofibers (NFs) for tissue engineering has been more developed. For this purpose, we fabricated the NFs (Zein/Polycaprolactone/Collagen) (Zein/PCL/Collagen) incorporated by zinc oxide NPs (ZnO NPs) and Aloe-vera (NFs/ZnO/Alv) using the electrospinning method. Prepared NFs were studied for their morphological, mechanical, thermal stability, and hydrophilic properties. Among the developed NFs, those loaded by ZnO (1 wt%) and Alv (8 wt%) and with Zein/PCL (70:30) displayed the suitable thermal stability and mechanical properties. The water contact angle of NFs improved by decreasing the Zein/PCL blending ratio. Cell culture results showed that the NFs had good cytocompatibility. The cell adhesion potential of this mats were certified with studying by fibroblast cells for various time intervals (24 h and 72 h). The NFs/ZnO/Alv sample revealed inhibition activity against S. aureus (19.23 ± 1.35 mm) and E. coli (15.38 ± 1.12 mm) bacteria. Thus, these results offered that the prepared NFs can be promised as an active scaffold for wound healing uses. • Aloe-vera (Alv)-loaded nanofibrous scaffold based on Zein/Collagen/PCL were successfully developed. • The developed NFs showed the suitable thermal stability and mechanical properties. • The water contact angle of NFs improved by decreasing the Zein/PCL blending ratio. • The developed NFs revealed inhibition activity against S. aureus and E. coli bacteria. • The cell culture results showed that the NFs showed good cytocompatibility. [ABSTRACT FROM AUTHOR]

Combining natural polysaccharides with synthetic materials improves their functional properties which are essential for designing sustained-release drug delivery systems. In this context, the Aloe vera leaf mucilage/hydrogel (ALH) was reacted with acrylic acid (AA) to synthesize a copolymerized hydrogel, i.e., ALH-grafted-Polyacrylic acid (ALH-g-PAA) through free radical copolymerization. Concentrations of the crosslinker N,N′ -methylene-bis-acrylamide (MBA), and the initiator potassium persulfate (KPS) were optimized to study their effects on ALH-g-PAA swelling. The FTIR and solid-state NMR (CP/MAS 13C NMR) spectra witnessed the formation of ALH-g-PAA. Scanning electron microscopy (SEM) analysis revealed superporous nature of ALH-g-PAA. The gel fraction (%) of ALH-g-PAA was directly related to the concentrations of AA and MBA whereas the sol fraction was inversely related to the concentrations of AA and MBA. The porosity (%) of ALH-g-PAA directly depends on the concentration of AA and MBA. The ALH-g-PAA swelled admirably at pH 7.4 and insignificantly at pH 1.2. The ALH-g-PAA offered on/off switching properties at pH 7.4/1.2. The metoprolol tartrate was loaded on different formulations of ALH-g-PAA. The ALH-g-PAA showed pH, time, and swelling-dependent release of metoprolol tartrate (MT) for 24 h following the first-order kinetic and Korsmeyer-Peppas model. Haemocompatibility studies ascertained the non-thrombogenic and non-hemolytic behavior of ALH-g-PAA. [Display omitted] • Synthesis of Aloe vera mucilage-based hydrogel by free radical polymerization • Copolymeric hydrogel appears a superporous and stimuli-responsive material • Hydrogel exhibits on-off switching at pH 7.4 and 1.2, respectively • The synthesized hydrogel shows pH-dependent swelling and drug release • Hydrogel exhibited non-hemolytic and non-thrombogenic potential [ABSTRACT FROM AUTHOR]

This study investigates the impact of water and salinity stress on Aloe vera , focusing on the role of Aloe vera polysaccharides in mitigating these stresses. Pectins and acemannan were the most affected polymers. Low soil moisture and high salinity (NaCl 80 mM) increased pectic substances, altering rhamnogalacturonan type I in Aloe vera gel. Aloe vera pectins maintained a consistent 60 % methyl-esterification regardless of conditions. Interestingly, acemannan content rose with salinity, particularly under low moisture, accompanied by 90 to 150 % acetylation increase. These changes improved the functionality of Aloe vera polysaccharides: pectins increased cell wall reinforcement and interactions, while highly acetylated acemannan retained water for sustained plant functions. This study highlights the crucial role of Aloe vera polysaccharides in enhancing plant resilience to water and salinity stress, leading to improved functional properties. [Display omitted] • The role of acemannan and pectin on the tolerance of Aloe vera to saline-water stress was studied. • Acemannan and pectic polysaccharides levels increase with higher saline-water stress. • Abiotic stress leads to increased acetylation of acemannan and branching of pectic polysaccharides. • Highly acetylated acemannan may improve intracellular water management during saline-water stress. • The presence of highly branched pectin as a result of abiotic stress could reinforce the cell wall. [ABSTRACT FROM AUTHOR]

Developing high-performance hydrogels with anti-freeze, and antimicrobial properties is crucial for the practical application of flexible sensors. In this study, we prepared silver nanoparticles (AgNPs) with aloe polysaccharide (AP) as a reducing agent. Then, the AP/AgNPs were added to a system of polyvinyl alcohol and borax crosslinked in water/glycerol to obtain a multifunctional conductive hydrogel. The incorporated AgNPs improved the conductivity (0.39 S/m) and mechanical properties (elongation at break: 732.9 %, fracture strength: 1267.6 kPa) of the hydrogel. In addition, resultant hydrogel exhibited potential for sensing strain, temperature, and humidity. When used as a strain sensor, the hydrogel system exhibited low detection limit (0.1 %), and fast response (0.08 s). The resistance of the hydrogel decreased with an increase in the absorbed moisture content, enabling humidity detection (25–95 %) to monitor breathing status. As a temperature sensor, the hydrogel supported a wide detection range (−50 to +90 °C) and sensitivity (−30–0 °C, temperature coefficient of resistance (TCR) = −5.64 %/°C) to detect changes in the ambient temperature. This study proposes a simple method for manufacturing multifunctional hydrogel sensors, which broadens their application prospects in wearable sensing and electronic products. [Display omitted] • Reduction of silver nanoparticles using aloe vera polysaccharides • Conductive hydrogels with excellent mechanical properties, anti–freeze and anti–bacterial properties • Minimum monitoring limit of 0.1 % as a strain sensor • Multi-functional sensors for human movement, respiration and temperature monitoring [ABSTRACT FROM AUTHOR]

Recently, various innovative advancements have been made in carbohydrate research to design versatile materials for biomedical applications. The current research focuses on the development of copolymeric hydrogel wound dressings (HWD) using a combination of aloe vera (AV) - sterculia gum (SG) - poly (vinylsulfonic acid) (VSA)-based with the aim to enhancing their efficacy in drug delivery (DD) applications. These hydrogel dressings were encapsulated with levofloxacin and lidocaine to address both microbial infection and pain. Copolymers were characterized by FESEM, SEM, EDS, AFM, 13C NMR, FTIR, XRD, and TGA-DTG analysis. Hydrogel exhibited a fluid absorption capacity of 4.52 ± 0.12 g per gram of polymeric dressing in simulated wound conditions. The hydrogels displayed a sustained release of drugs, demonstrating a non-Fickian diffusion mechanism. Polymer dressings revealed antibacterial, mucoadhesive, antioxidant, biocompatible and non-cytotoxic properties. Additionally, HWD displayed permeability to O 2 and water vapour, yet was impermeable to microbial penetration. Overall, the findings of physiological, biochemical and drug delivery properties demonstrated the suitability of materials for wound dressing applications. [ABSTRACT FROM AUTHOR]

In recent years, there increment demand for healthier food options that can replace high-fat ingredients in bakery products without compromising their taste and texture. This research was focused on a formulation study of the blend of nano polysaccharides derived from aloe vera and guar gum at various concentrations. This study selected the blend concentration of 1 % aloe vera mucilage (AM) and 1 % guar gum (GG) due to its optimal gelling properties. Different magnetic stirring time durations were employed to formulate AGB (aloe vera guar gum blend). The particle size of AGB revealed the lowest nanoparticle size (761.03 ± 62 nm) with a stirring time of 4 h. The FTIR analysis found the presence of monomer sugars in AGB nano polysaccharide powder such as mannose, arabinose, and glucose. The thermogram results displayed an endothermic peak for all samples with a glass transition temperature (Tg) between 16 and 50 °C. The SEM image of the AGB indicated uniform spherical particles. The AGB powder exhibited good functional properties. The antimicrobial activity of AGB powder against Staphylococcus aureus , Escherichia coli , and Candida albicans was 22.32 ± 0.02, 21.56 ± 0.02, and 19.33 ± 0.33 mm, respectively. Furthermore, the effects of different levels of vegetable fat replacement with AGB powder on cake sensory properties, thermal stability, and texture characteristics were also examined. Notably, the cake containing a 50 % substitution of vegetable fat with AGB (C50) supplied desirable physicochemical, textural, and sensory properties. These results can provide advantages for the development of fat replacers in bakery products. [Display omitted] • Spray-dried aloe vera and guar gum powder demonstrated a nanoscale particle size. • FTIR analysis identified mannose, arabinose, and glucose in the sample. • Aloe vera and guar gum blend powder revealed higher thermal stability. • Cake with 50 % less fat has similar taste acceptance as the standard cake. [ABSTRACT FROM AUTHOR]

The aim of our study is to provide new type skeleton of α-glucosidase inhibitor from food source. A new 6′- O -(E)–cinnamoyl-7-methoxy-aloin A (1) was isolated from Aloe vera. Also, known chysalodin (2) was the first report for A. vera. These structures were identified with based on HR-ESI Mass and 1/2D-NMR spectra. These isolated compounds were tested for their inhibitory activities against α-glucosidase by using spectrophotometer. Of these, compound 2 exhibited inhibitory activity, with an IC 50 value of 13.4 ± 1.5 μM. An enzyme kinetic study identified the mechanism of binding of the ligand with the enzyme; the ligand binds in the active site of the enzyme in a competitive mode. Additionally, fluorescence quenching between the ligand and receptor revealed a two-to-one reaction. Finally, this finding provides that anthraquinone dimer (2) could be a starting point for the design of new class of α-glucosidase inhibitor. • A new 6′- O -(E)–cinnamoyl-7-methoxy-aloin A (1), and chysalodin (2) were isolated from Aloe vera for the first time. • Chysalodin (2) was bound into α-glucosidase with IC 50 value of 13.4 ± 1.5 μM. • Through steady-state kinetic study, this was revealed with competitive of mode for inhibition. • Fluorescence quenching led to calculate the kinetic parameters of compound 2. [ABSTRACT FROM AUTHOR]

Spirograph is a geometric drawing that produces mathematical roulette curves of the variety technically known as hypotrochoids and epitrochoids. This paper presents a research on a novel Spirograph Based Mechanical System (SBMS) meant for the production of electrospun mat with near uniform characteristics for wound dressing applications. A combination of natural (Chitosan) and artificial (Polyethylene oxide) polymer, along with Aloe vera plant extract has been evaluated as a material for wound dressing application. The improvement in the mechanical property, in vitro and in vivo studies indicate developed SBMS compared to the typical collectors has been found suitable for producing electrospun mat for wound dressing applications. [ABSTRACT FROM AUTHOR]

Gelatin-based hydrogels have gained considerable attention due to their resemblance to the extracellular matrix and hydrophilic three-dimensional network structure. Apart from providing an air-permeable and moist environment, these hydrogels optimize the inflammatory microenvironment of the wounds. These properties make gelatin-based hydrogels highly competitive in the field of wound dressings. In this study, a series of composite hydrogels were prepared using gelatin (Gel) and carboxymethyl chitosan (CMCh) as primary materials, glutaraldehyde as a crosslinker, and aloe vera juice as an anti-inflammatory component. The properties of the hydrogel, including its rheological properties, microscopic structures, mechanical properties, swelling ratios, thermal stability, antibacterial properties, and biocompatibility, were investigated. The results demonstrate that the gelatin-based hydrogels exhibit good elasticity and rapid self-healing ability. The hydrogels exhibited slight shear behavior, which is advantageous for skin care applications. Furthermore, the inclusion of aloe vera juice into the hydrogel resulted in a dense structure, improved mechanical properties and enhanced swelling ratio. The Gel/CMCh/Aloe hydrogels tolerate a compressive strength similar to that of human skin. Moreover, the hydrogels displayed excellent cytocompatibility with HFF-1 cells, and exhibited antibacterial activity against E. coli and S. aureus. Lomefloxacin was used as a model drug to study the releasing behavior of the Gel/CMCh/aloe hydrogels. The results showed that the drug was released rapidly at the initial stage, and could continue to be released for 12 h, the maximum releasing rate exceeded 20 %. These findings suggest that the gelatin-based hydrogels hold great promise as effective wound dressings. [ABSTRACT FROM AUTHOR]

Herein, the hydrogel from the leaf of the Aloe vera plant (ALH) was succinylated (SALH) and saponified (NaSALH). The FTIR, solid-state CP/MAS 13C NMR, and SEM-EDX spectroscopic analyses witnessed the formation of SALH and NaSALH from ALH. The pH ZPC for NaSALH was found to be 4.90, indicating the presence of −ve charge on its surface. The Cd2+ sorption efficiency of NaSALH was found to be dependent on pH, NaALH dose, Cd2+ concentration, contact time, and temperature. The maximum Cd2+ removal from DW and HGW was found to be 227.27 and 212.77 mg g−1 according to the Langmuir isothermal model (>0.99) at pH of 6, NaSALH dose of 40 mg g−1, Cd2+ concentration of 90 mg L−1, contact time of 30 min, and temperature of 298 K. The kinetic analysis of Cd2+ sorption data witnessed that the Cd2+ removal by chemisorption mechanism and followed pseudo-second-order kinetics (>0.99). The −ve values of ΔG° and ΔH° assessed the spontaneous and exothermic nature of sorption of Cd2+ by NaSALH. The regeneration and sorption/desorption studies indicated that the sorbent NaSALH is regenerable. • Isolation of mucilage from Aloe vera • Synthesis of sodium salt of succinate bonded A. vera hydrogel (NaSALH). • Evaluation of cadmium removal capacity of NaSALH from DW and HGW • Cd2+ sorption data fitted well to Langmuir isotherm, pseudo second-order kinetics, and ion-exchange models. • NaSALH appeared supersorbent and regenerable. [ABSTRACT FROM AUTHOR]

Raft-forming systems are designed to relieve reflux symptoms by forming a physical barrier on top of the stomach. The present study aimed to evaluate the physico-chemical properties of alginate- aloe vera raft-forming systems for the first time. To achieve this goal, aloe vera was used in the proportion of 1 and 1.5 % in raft suspensions containing 5 % alginate as the main component of gel structure. Rafts were characterized by their volume, floating behavior, thickness, swelling properties, strength, resilience, reflux resistance, and acid neutralization capacity (ANC). Results showed the effectiveness of aloe vera in forming rafts that were voluminous, buoyant with greater total floating time (TFT), and stronger than formulations with no aloe vera. Furthermore, data showed that the presence of aloe vera could improve resilience time, swelling proportions, resistance to reflux under simulant conditions of movement in the stomach, and ANC values of rafts. Rafts were further characterized by oscillatory strain sweep test, differential scanning calorimetry, and Fourier transform infrared spectroscopy. Evaluation of the mechanical properties of rafts displayed a viscoelastic behavior of gels corresponding to the internal cross-linked structure of rafts. This study demonstrated that designing of alginate- aloe vera rafts can be suitable for the treatment of gastro-esophageal reflux disorders. • The combination of alginate with Aloe vera affected raft structure and its characteristics. • The presence of Aloe vera could improve strength, resistance to reflux, and other related properties of rafts under simulant conditions in stomach. • Alginate- Aloe vera based rafts were cohesive and viscoelastic, corresponding to the internal cross-linked structure. • Alginate- Aloe vera rafts showed suitable potential for the treatment of gastro-esophageal reflux disorders. [ABSTRACT FROM AUTHOR]

Abstract Aloe barbadensis Miller (Aloe vera) is a kind of popular medicinal and horticultural crops widely cultivated around the world. Polysaccharides with different structural features and bioactivities have been isolated from the pulp of Aloe vera. In the current study, a novel polysaccharide fraction (AGP 40) isolated and purified from gel juice of Aloe vera was subjected to structural and conformational analysis. AGP 40 had a backbone consisting of major →4)-β-Man p -(1 → residues and minor →3)-β-Man p -(1 → and →4)-β-Glc p -(1 → residues. Low degree of branches distributed at O -6 of 4-linked mannose in the main chain. The acetyl groups in AGP 40 presented as mono- or di-substituents (O -2, O -3, O -6, O -2,3, O -2,6 and O -3,6). AGP 40 performed as random coil conformation in 0.1 M NaNO 3 solution, and had fibrous filaments and irregular spherical structures in solid state. Detailed structural and conformational analysis of AGP 40 in the present study may greatly improve the bioactive function investigation and mechanism explanation of acetyl glucomannans in the future. Highlights • Polysaccharide (AGP 40) from gel of Aloe vera was typical O -acetyl-glucomannan. • Acetyl groups linked as mono−/di-substituents along the β-1,4-glucomannan backbone. • AGP 40 (1.0 mg/mL) adopted random coil conformation in 0.1 M NaNO 3 solution. • AGP 40 performed fibrous or ribbon-like morphology in solid state. [ABSTRACT FROM AUTHOR]

Abstract Scar preventive dextran based bionanocomposite dressings containing aloe vera (AV) and manuka honey (MH) were developed as wound care devices. This work was a challenge to fabricate herbal dressing that promotes healing, which at the same time is biocompatible, non-toxic, biodegradable, and cost effective in terms of the simplicity of application in complex chronic wound situations. With this aim, we synthesized in - situ crosslinked dextran/nanosoy/glycerol/chitosan (DNG/Ch) nanocomposite membranes via solvent casting technique followed by subsequent addition of AV and MH to obtain DNG/Ch/AV and DNG/Ch/MH herbal dressings. The drug release kinetics of the bionanocomposite dressings indicated an initial burst release of AV and MH, followed by controlled release when examined in - vitro using non-fickian and quasi-fickian model. Antibacterial studies confirmed >99% antibacterial activity against both Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) and bacterial adherence test demonstrated its efficacy for arresting microbial invasion. Wound healing analysis conducted in - vivo showed enhanced epithelialization in the terms scar prevention and aesthetics with absolute wound contraction for the mice treated with AV loaded dressings after 14th post wounding day. Histological features displayed ordered deposition of collagen with a thin epidermis layer. Hence, the present herbal dressing could function without many cytotoxicity and biocompatibility issues. Graphical abstract Unlabelled Image Highlights • Bionanocomposite dressings of dextran/nanosoy/chitosan with aloe vera and manuka honey were fabricated. • Dressings exhibited complete inhibition of bacterial infection. • Rapid healing with diminished scar formation was demonstrated with aloe vera dressings. • Drug release mechanism and kinetics indicated long term effectiveness of dressings at the wound site. [ABSTRACT FROM AUTHOR]

Abstract In this study, firstly, gelation process and its optimization at different pH (3–7) and concentration (0.2–1.6%, w/v) on the viscoelastic behavior of reconstituted Aloe vera (A. vera) samples were studied using response surface methodology (RSM). The optimum condition obtained by numerical optimization for the formulation of reconstituted samples was 1.6%, w/v at pH 7 with desirability of 0.84. Secondly, structural alteration of the reconstituted A. vera gels were evaluated using FTIR, SEM, and TEM at particular pH (3–7) and concentration (0.2–1.6%, w/v). The FTIR spectrum of reconstituted hydrogels showed formation of new hydrogen bond at 3600–3000 cm−1 and deacetylation of acemannan at 1740 cm−1 with increase in A. vera concentration. Moreover, SEM and TEM micrograph images of A. vera xerogel at lower concentrations (0.2–0.4%, w/v) evidenced the intact surface morphology and formation of clumps and at higher concentration (0.8–1.6%, w/v), exhibited rope-like network patterns and composed of spherical aggregates. Highlights • Effect of concentration and pH on reconstituted A. vera gel formation was studied. • 1.6% w/v of A. vera concentration at pH 7 was the optimized condition. • Spherical like pattern was more pronounced in optimized reconstituted gel. • Hydrogen & electrostatic bonds formation in the reconstituted gels were confirmed. [ABSTRACT FROM AUTHOR]

Vinpocetine (VIN), a derivative of vincamine found in the vinca plant, widens blood vessels in the brain and has been shown to improve cognitive function, memory, and cerebrovascular disorders. Nevertheless, the clinical utility of VIN is constrained by factors such as low oral bioavailability owing to the first-pass metabolism that often demands frequent dosing of 3–4 tablets/day. In this regard, the present work aimed to develop VIN-loaded chitosan nanoparticles (VIN-CH-NPs) to surmount these limitations and in view to enhance delivery to the brain of VIN by minimizing systemic exposure. The chitosan (CH) nanoparticles (NP) were developed by ionotropic gelation technique employing tripolyphosphate (TPP) as a cross-linking agent. Employing Design of Experiments (DoE), the effect of CH and TPP concentrations and stirring speed were systematically optimized using Box Behnken design (BBD). The optimized batch of nanoparticles displayed a particle size, zeta potential, entrapment efficiency, and drug loading of 130.6 ± 8.38 nm, +40.81 ± 0.11 mV, 97.56 ± 0.04 %, and 61 ± 0.89 %, respectively. Fourier Transform Infrared Spectroscopy indicated the chemical integrity of the drug ruling out the interaction between the VIN and excipients used. DSC and PXRD data indicated that reduction of the crystallinity of VIN in the chitosan matrix. These VIN-CH-NPs manifested good stability, exhibiting an almost spherical morphology. To mitigate rapid mucociliary clearance upon intranasal administration, the optimized VIN-CH-NPs were incorporated into thermosensitive in situ gel (VIN-CHN-ISG). It was observed that the in-situ gel loaded with nanoparticles was opalescent with a pH level of 5.3 ± 0.38. It was also noted that the gelation temperature was 32 ± 0.89 °C, and the gelation time was approximately 15 s. The drug delivery to the brain through the nasal application of optimized VIN-NPs in situ gel was assessed in rats. The results indicated significant nasal application of the in-situ gel nearly doubled the C max (P < 0.05) and AUC 0-t (P < 0.05) in the brain compared to oral administration. Nasal administration improved drug delivery to the brain by reducing systemic exposure to VIN. A histopathological study of the nasal mucosa revealed no irritation or toxicity, making it safe for nasal administration. These findings suggest that the developed NPs in-situ gel effectively targeted vinpocetine to the brain through the nasal pathway, providing a potential therapeutic strategy for managing Alzheimer's disease. [Display omitted] [ABSTRACT FROM AUTHOR]

The chondrogenic efficacy of aloe vera blended collagen-chitosan (COL-CS-AV) porous scaffold was investigated using articular chondrocytes in a standard condition. Cytocompatibility was analyzed using fluorescent dyes (calcein AM/ethidium bromide) and the viable cells were quantified by MTT assay. Glycosaminoglycan (GAG) content of ECM was estimated by using 1, 9-Dimethyl methylene Blue (DMMB). The total RNA content was quantified and the cartilage specific genes (col2a1, Acan) were amplified by reverse transcription-PCR from the cell lysate of the scaffolds. Histological examination was made using Haematoxylin and Eosin (H&E), safranin-O, masson's trichrome, alcian blue, and alizarin red to stain the specific component of ECM secreted on the construct. The cartilage specific collagen type II was estimated by immunohistochemistry using monoclonal type II collagen antibody. The results of these studies proved that COL-CS-AV scaffold has more chondrogenic efficacy than COL-CS, thus the aloe vera blend COL-CS-AV scaffold might be used as suitable candidate for cartilage tissue engineering. [ABSTRACT FROM AUTHOR]

The crosslinked sodium alginate/mucilage/ Aloe vera /glycerin was optimized by different ratios of each factor to be an absorption wound dressing base for infected wound healing. Mucilage was extracted from seeds of Ocimum americanum. The Box-Behnken design (BBD) in response surface methodology (RSM) was used to construct an optimal wound dressing base with the target ranges of mechanical and physical properties of each formulation. The independent variables selected were sodium alginate (X 1 : 0.25–0.75 g), mucilage (X 2 : 0.00–0.30 g), Aloe vera (X 3 : 0.00–0.30 g), and glycerin (X 4 : 0.00–1.00 g). The dependent variables were tensile strength (Y 1 : low value), elongation at break (Y 2 : high value), Young's modulus (Y 3 : high value), swelling ratio (Y 4 : high value), erosion (Y 5 : low value), and moisture uptake (Y 6 : high value). The results showed that the wound dressing base with the most desirable response consists of sodium alginate (59.90 % w/w), mucilage (23.96 % w/w), and glycerin (16.14 % w/w) without Aloe vera gel powder (0.00 % w/w). [Display omitted] [ABSTRACT FROM AUTHOR]

Biopolymer-based food packaging films are gaining increasing popularity, as consumers' demands for sustainable alternatives and environmental concerns associated with synthetic plastic packaging grow. In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities. The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films. The EuNE droplet size was about 200 nm, and they were uniformly distributed throughout the film matrices. Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency. The XRD spectra of the fabricated films showed good compatibility between the chitosan and the incorporated active agents. The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively. • Chitosan is a functional biopolymer used for fabrication of composite film. • Chitosan was blended with active agents like EuNE, AVG and ZnONPs. • The composite films had superior UV barrier and antimicrobial properties. • Blending of AVG and ZnONPs reduced release of EuNE from the composite films. • The developed composite films can be sustainable alternatives to synthetic plastics. [ABSTRACT FROM AUTHOR]

We prepared nanocomposite (XG-AVE-Ag/MgO NCs) using the bimetallic Ag/MgO NPs, Aloe vera extract (AVE), and biopolymer (xanthan gum (XG)) to archive a synergetic antibacterial and wound healing activity. The changes in XRD peaks at 20° of XG-AVE-Ag/MgO NCs indicated the XG encapsulation. The XG-AVE-Ag/MgO NCs showed the zeta potential and zeta size of 151.3 ± 3.14 d.nm and −15.2 ± 1.08 mV with a PDI of 0.265 while TEM showed an average size of 61.19 ± 3.89 nm. The EDS confirmed the co-existence of Ag, Mg, carbon, oxygen, and nitrogen in NCs. XG-AVE-Ag/MgO NCs displayed higher antibacterial activity in terms of zone of inhibition, at 15.00 ± 0.12 mm for B. cereus and 14.50 ± 0.85 mm for E. coli. Moreover, NCs exhibited MICs of 2.5 μg/mL for E. coli , and 0.62 μg/mL for B. cereus. The in vitro cytotoxicity and hemolysis assays indicated the non-toxic properties of XG-AVE-Ag/MgO NCs. The higher wound closure activity was observed with the treatment of XG-AVE-Ag/MgO NCs (91.19 ± 1.87 %) compared to the control, untreated group (68.68 ± 3.54 %) at 48 h of incubation. These findings revealed that XG-AVE-Ag/MgO NCs was promising, non-toxic, antibacterial, and wound-healing agent that deserved further in-vivo studies. [Display omitted] • We prepared and characterized the biopolymer nanocomposites (XG-AVE-Ag/MgO NCs). • The XG-AVE-Ag/MgO NCs were a spherical shape, sized <200 nm with good stability. • XG-AVE-Ag/MgO NCs inhibited E. coli and B. cereus and triggers wound closure activity. • XG-AVE-Ag/MgO NCs was non-toxic, hemocompatible to NIH3T3, HEK 293 and RBCs [ABSTRACT FROM AUTHOR]

Psoriasis is a disease that causes keratinocytes to proliferate ten times faster than normal, resulting in chronic inflammation and immune cell infiltration in the skin. Aloe vera (A. vera) creams have been used topically for treating psoriasis because they contain several antioxidant species; however, they have several limitations. Natural rubber latex (NRL) has been used as occlusive dressings to promote wound healing by stimulating cell proliferation, neoangiogenesis, and extracellular matrix formation. In this work, we developed a new A. vera -releasing NRL dressing by a solvent casting method to load A. vera into NRL. FTIR and rheological analyzes revealed no covalent interactions between A. vera and NRL in the dressing. We observed that 58.8 % of the loaded A. vera , present on the surface and inside the dressing, was released after 4 days. Biocompatibility and hemocompatibility were validated in vitro using human dermal fibroblasts and sheep blood, respectively. We observed that ~70 % of the free antioxidant properties of A. vera were preserved, and the total phenolic content was 2.31-fold higher than NRL alone. In summary, we combined the antipsoriatic properties of A. vera with the healing activity of NRL to generate a novel occlusive dressing that may be indicated for the management and/or treatment of psoriasis symptoms simply and economically. [Display omitted] • The occlusive dressing released 58.8 % of the Aloe vera loaded after 4 days. • The occlusive dressing was biocompatible and considered safe. • These dressings preserved ~70 % of the antioxidant properties of free Aloe vera. • These dressings exhibited phenolic contents 2.31-fold higher than latex rubber. • These dressings manage psoriasis symptoms simply and economically. [ABSTRACT FROM AUTHOR]

The objective of this project is to create a unique type of polymer known as an ion imprinted polymer (IIP) and a non-imprinted polymer (NIP) utilizing natural waste biosorbent materials. One example of this type of waste is Aloe vera , a plant with many medicinal uses that is grown globally. Aloe vera is considered one of the most valuable medicinal plants with a wide range of applications. Extracted Aloe vera was used as functional monomers for the first time to prepare new IIPs, epichlorohydrin, and Cu2+ ion as the cross-linking agent and template, respectively. The NIP was also synthesized for comparison, without the use of the Cu2+ salt. Following polymerization, the IIP particles were cleansed of template ions through a 0.1 M EDTA leaching process, resulting in the formation of cavities within the particles, these cavities in the polymer provide selective linking zones for these specific template ions. The synthesized IIPs were characterized using the most recent identification instruments. The experimental parameters for adsorption, such as pH of a solution, contact time, initial copper concentration, adsorbent dosage, and temperature have been optimized. The most effective conditions for metal adsorption onto the ionic imprinted polymer were found to be a pH of 8.0, a temperature of 30 °C, a concentration of 0.03 g/100 mL, and a contact time of 50 min. Based on the ANOVA statistical value, the adsorption of Cu2+ ion on IIP is significant with very low probability (p) values (<0.001). The Langmuir isotherm model and a second-order reaction were both used in the adsorption process. According to thermodynamic characteristics, Cu2+ adsorption over IIPs and NIP was an endothermic, spontaneous process. Compared to NIP, the imprinted polymer exhibits a significantly better capacity and selectivity for Cu2+ adsorption, the maximum removal percentage of IIPs and NIP was 96.02 % and 74.3 % respectively. Moreover, the research showed that ion imprinting can be a promising technique for preparing selective adsorbents to separate and preconcentrate metal in a medium of multiple competitive metals (Co2+, Cd2+, Ni2+, Zn2+, Fe2+, and Pb2+) The most important point for this new Cu2+-IIPs was shown superior reusability up to 8 cycles with small decrees in uptake capability. [Display omitted] [ABSTRACT FROM AUTHOR]

Wound healing is an extremely intricate process involving various potential factors that can contribute towards delayed healing, one of them being bacterial colonization. The current research addresses this issue through the development of herbal antimicrobial films that can be stripped off easily, formed using an essential oil component thymol, biopolymer chitosan, and herbal plant Aloe vera. In comparison to the conventionally used nanoemulsions, thymol encapsulated in chitosan- Aloe vera (CA) film exhibited high encapsulation efficiency (95.3 %) with alleviated physical stability, as established using a high zeta potential value. The pronounced loss of crystallinity, validated using X-ray diffractometry, combined with the results obtained from Infrared and Fluorescence spectroscopic analysis, confirmed the encapsulation of thymol in CA matrix through hydrophobic interactions. This encapsulation increases the spaces between biopolymer chains facilitating greater intrusion of water, conducive for preventing the possibility of bacterial infection. Antimicrobial activity was tested against various pathogenic microbes such as Bacillus , Staphylococcus , Escherichia , Pseudomonas , Klebsiella and Candida. Results showed potential antimicrobial activity in the prepared films. Release test was also run at 25 °C suggesting a two-step biphasic release mechanism. The encapsulated thymol had higher biological activity, as assessed by antioxidant DPPH assay, likely due to improved dispersibility. [Display omitted] [ABSTRACT FROM AUTHOR]

Impairment of intestinal epithelium barrier is a hallmark of gut pathology. Cell death can compromise barrier function and impair epithelial restitution directly or indirectly in inflammatory bowel disease (IBD). Our previous work demonstrated that glucomannan from Aloe vera gel (AGP) protected mice from DSS-induced colitis, with unclear mechanism of AGP-intestinal barrier interactions. Here, AGP maintained the integrity of intestinal barrier in colitis mice. RNA-Sequencing results indicated that pathways related to anoikis (apoptosis induced by loss of cell-matrix interaction), mitochondrial function and oxidative stress were significantly altered in the process of AGP-intestinal barrier interaction. Further experiments confirmed that AGP activated Nrf2, decreased ROS levels, mitigated mitochondrial dysfunction and anoikis of colonic epithelial cells in mice. Intriguingly, AGP reversed oxidative stress and mitochondrial dysfunction induced by knockdown or inhibitor (ML385) of Nrf2 in IEC-6 cells, which indicated the essential role of Nrf2-mitochondrial axis in the intestinal protective function of AGP. In addition, AGP alleviated anoikis caused by impaired mitochondrial function. Hence, this current work indicated that AGP might maintain intestinal barrier integrity by mitigating anoikis mediated by Nrf2-mitochondria axis. These findings provide new evidence into the effect of polysaccharides maintaining intestinal barrier integrity. • Aloe vera gel polysaccharide (AGP) maintains intestinal barrier integrity. • The effect of AGP was related to oxidative stress, mitochondria and anoikis. • AGP reduced ROS levels via activating the Nrf2/Gpx2 cascade. • AGP maintained mitochondrial function through activating Nrf2. • AGP mitigated anoikis dependent on mitochondrial pathway of caspase activation. [ABSTRACT FROM AUTHOR]

Application of natural materials in wound healing is an interest topic due to effective treatment with no side effects. In this paper, Aloe Vera extract was encapsulated into Tragacanth Gum through a sonochemical microemulsion process to prepare a wound healing product. FESEM/EDX and FT-IR proved the successfully formation of the nanocapsules with spherical shape by cross-linking aluminum ions with Tragacanth Gum . The therapeutic characteristics of the prepared wound healing product were investigated using antimicrobial, cytotoxicity and wound healing assays. Relative high antimicrobial activities with the microbial reduction of 84, 91 and 80% against E. coli , S. aureus and C. albicans , a cell viability of 98% against human fibroblast cells and a good wound healing activity with considerable migration rate of fibroblast cells are the important advantages of the new formed wound healing product. [ABSTRACT FROM AUTHOR]

Biologically active scaffolds used in tissue engineering and regenerative medicine have been generating promising results in skin replacement. The present study aims to test the hypothesis that the incorporation of Aloe vera and snail mucus into scaffolds based on gelatin and chitosan could improve their structure, composition and biodegradability, with a potential effect on bioactivity. Homogeneous pore diameter as well as pore walls in the composite scaffold could be seen in the SEM image. The pores in the scaffolds were interconnected and their sizes ranged from 93 to 296 μm. The addition of Aloe vera and snail mucus enlarged the mean pore size with increased porosity and caused changes in the pore architecture. The FTIR analysis has shown good affinity and interaction between the matrix and the Aloe, which may decrease water-binding sites, so this fact hindered the water absorption capacity of the material. The mechanical properties could explain the highest swelling capacity of the snail scaffold, because the high percentage of elongation could facilitate the entry of liquid in it, generating a matrix with plenty of fluid retention. The real innovation in the present work could be the use of these substances (Aloe and snail mucus) for tissue engineering. [ABSTRACT FROM AUTHOR]

Aloe vera and curcumin loaded oxidized pectin–gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism – erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management. [ABSTRACT FROM AUTHOR]

Abstract: Significant wound healing activity of Aloe vera (AV) and higher elastic strength of Silk fibroin (SF) along with mammalian cell compatibility makes AV and SF an attractive material for tissue engineering. The purpose of the present work was to combine their unique properties, with the advantage of electrospinning to prepare a hybrid transdermal biomaterial for dermal substitutes. The physico-chemical characterization of the developed scaffold showed finer morphology expressing amino and esteric groups with improved hydrophilic properties and favorable tensile strain of 116% desirable for skin tissue engineering. Their biological response showed favorable fibroblast proliferation compared to control which almost increased linearly by (p <0.01) 34.68% on day 3, (p <0.01) 19.13% on day 6, and (p <0.001) 97.86% on day 9 with higher expression of CMFDA, collagen and F-actin proteins. The obtained results prove that the nanofibrous scaffold with synergistic property of AV and SF would be a potential biomaterial for skin tissue regeneration. [Copyright &y& Elsevier]

Highlights: [•] Aloe vera (Aloe barbadensis) polysaccharides (AVP) were isolated and characterized. [•] Open wounds of rats were daily treated with different doses of AVP for 30 days. [•] AVP enhanced wound closure in treated animals. [•] AVP upregulates MMP-3 and TIMP-2 gene expression in wounded skins 15 days post treatment. [•] AVP increased the level of collagen and aminosugar synthesis in repaired wounds. [ABSTRACT FROM AUTHOR]

Abstract: Alginate and Aloe vera are natural materials widely investigated and used in the biomedical field. In this research work, thin hydrogel films composed by alginate and Aloe vera gel in different proportions (95:5, 85:15 and 75:25, v/v) were prepared and characterized. The films were evaluated regarding the light transmission behavior, contact angle measurements, and chemical, thermal and mechanical properties. These thin hydrogel films, prepared by crosslinking reaction using 5% calcium chloride solution, were also investigated relatively to their water solubility and swelling behavior. Results showed that Aloe vera improved the transparency of the films, as well their thermal stability. The developed films present adequate mechanical properties for skin applications, while the solubility studies demonstrated the insolubility of the films after 24h of immersion in distilled water. The water absorption and swelling behavior of these films were greatly improved by the increase in Aloe vera proportion. [Copyright &y& Elsevier]

Abstract: The Aloe protein of 14kDa from the Aloe vera leaf gel was isolated by an ion exchange chromatography using DEAE-cellulose and CM-cellulose column. The purified Aloe protein exhibited a potent anti-fungal activity against Candida paraprilosis, Candida krusei and Candida albicans. In addition, the purified Aloe protein also showed an anti-inflammatory property against pure lipoxygenase and cyclooxygenase-2 with 84% and 73% inhibition, respectively, and was verified by binding with these proteins by real time method by the phenomenon of surface plasmon resonance. This Aloe protein is a novel protein possessing antifungal and anti-inflammatory properties and thus sets a platform to be used as a medicinal plant product. [Copyright &y& Elsevier]