1. Knockdown of UGT352A5 decreases the thiamethoxam resistance in Bemisia tabaci (Hemiptera: Gennadius).
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Du, Tianhua, Fu, Buli, Wei, Xuegao, Yin, Cheng, Yang, Jing, Huang, Mingjiao, Liang, Jinjin, Gong, Peipan, Liu, Shaonan, Xue, Hu, Hu, Jinyu, Diao, Yongchao, Gui, Lianyou, Yang, Xin, and Zhang, Youjun
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SWEETPOTATO whitefly , *THIAMETHOXAM , *INTEGRATED pest control , *URIDINE diphosphate , *HEMIPTERA , *INSECTICIDES , *INSECTICIDE resistance - Abstract
Uridine diphosphate (UDP)-glycosyltransferases (UGTs), which are major phase II detoxification enzymes, have been implicated in the glycosylation of lipophilic endobiotics and xenobiotics and thus potentially lead to the evolution of insecticide resistance. In this study, we identified and cloned two putative UGT genes from transcriptome data which are named UGT352A4 and UGT352A5. As demonstrated by qRT-PCR, two UGT genes were over-expressed in the thiamethoxam-resistant (THQR) strain relative to the susceptible (THQS) strain. Moreover, the induction experiment revealed that the expression of the UGT352A5 gene was significantly increased following exposure to thiamethoxam in the THQR strain. Furthermore, the expression of both UGT352A4 and UGT352A5 was downregulated after RNA interference, whereas only the silencing of UGT352A5 resulted in a noticeable increase in the mortality of THQR adults. Our results represent the first line of evidence showing that UGT352A5 might be responsible for conferring thiamethoxam resistance in B. tabaci. The results will be shed new insights for obtaining a better understanding of the role of UGTs in the evolution of insecticide resistance and developing new insect resistance management tactics within the sustainable integrated pest management framework. • Two UGT genes whether function to thiamethoxam resistance were comparative verified. • Over-expressed UGT352A4 does not involve in thiamethoxam resistance in B. tabaci. • Silenced the UGT352A5 increased the sensitivity of B. tabaci to thiamethoxam • UGT352A5 expression level increased with the increasing time of thiamethoxam exposure. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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