1. FOXO1 regulates the formation of bovine fat by targeting CD36 and STEAP4.
- Author
-
Song, Yaping, Zhang, Jiupan, Jiang, Chao, Song, Xiaoyu, Wu, Hao, Zhang, Juan, Raza, Sayed Haidar Abbas, Zhang, Le, Zhang, Lingkai, Cai, Bei, Wang, Xingping, Reng, Zhuoma Luo, Ma, Yun, and Wei, Dawei
- Subjects
- *
BOS , *FORKHEAD transcription factors , *FAT , *SITE-specific mutagenesis , *BIOSYNTHESIS , *PI3K/AKT pathway - Abstract
Intramuscular fat content is closely related to the quality of beef, where the forkhead box protein O1 (FOXO1) is involved in adipocyte differentiation and lipid metabolism, but the specific mechanism of its involvement is still unclear. In this study, interfering with FOXO1 promoted the G1/S transformation of bovine adipocytes by enhancing the expression of proliferation marker genes PCNA , CDK1 , CDK2 , CCNA2 , CCNB1 , and CCNE2 , thereby positively regulating the proliferation of bovine adipocytes. Additionally, interfering with FOXO1 negatively regulated the expression of adipogenic differentiation marker genes PPARG and CEBPA , as well as lipid anabolism marker genes ACC , FASN , SCD1 , SREBP1 , FABP4 , ACSL1 , LPL , and DGAT1 , thus reducing triglyceride (TG) content and inhibiting the generation of lipid droplets in bovine adipocytes. A combination of transcriptomic and metabolomics analyses revealed that FOXO1 could regulate the lipogenesis of cattle by influencing the AMPK and PI3K/AKT pathways. Importantly, chromatin immunoprecipitation (ChIP) and site-directed mutagenesis revealed that FOXO1 could regulate bovine lipogenesis by binding to the promoter regions of the CD36 and STEAP4 genes and affecting their transcriptional activities. These results provide a foundation for studying the role and molecular mechanism of FOXO1 in the bovine adipogenesis. • Interfering with bovine FOXO1 positively regulates the proliferation of bovine adipocytes. • Interfering with FOXO1 inhibits the generation of lipid droplets in bovine adipocytes by PPARG and CEBPA. • FOXO1 could regulate lipogenesis by influencing the activity of AMPK and PI3K/AKT pathways. • FOXO1 can regulate the lipogenesis by affecting the transcriptional activity of CD36 and STEAP4. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF