1. Effect of metal ions on the enzymatic hydrolysis of hemp seed oil by lipase Candida sp. 99-125
- Author
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Pei Wang, Limin Hao, Zhaodi Ke, Xin Liu, Qiaozhen Kang, and Jike Lu
- Subjects
inorganic chemicals ,0106 biological sciences ,Metal ions in aqueous solution ,01 natural sciences ,Biochemistry ,Absorbance ,Fungal Proteins ,Hydrolysis ,Ultraviolet visible spectroscopy ,Structural Biology ,010608 biotechnology ,Enzymatic hydrolysis ,Plant Oils ,Thermal stability ,Lipase ,Molecular Biology ,Candida ,Cannabis ,biology ,010405 organic chemistry ,Chemistry ,General Medicine ,0104 chemical sciences ,Metals ,Seeds ,biology.protein ,Selectivity ,Nuclear chemistry - Abstract
In order to study the effect of metal ions on the enzymatic hydrolysis of hemp seed oil by Candida sp. 99-125, the spectroscopy, stability and hydrolytic activity of the biocatalyst were investigated in presence of Ca2+, Mg2+, Fe2+, Fe3+, Cu2+, Sn2+, Pb2+, Zn2+ and Ba2+ metal ions, respectively. The UV spectroscopy showed that all the metal ions enhanced the absorbance but the decrease of fluorescence intensity was observed. All the metal ions could improve the lipase thermal stability except Cu2+ and Ba2+. Hydrolysis of hemp seed oil proved that Ca2+, Fe3+, Pb2+ and Ba2+ could significantly improve the hydrolytic rate, and metal ions could influence lipase selectivity. The study revealed that metal ions could improve lipase stability, hydrolysis activity in the hydrolytic process of hemp seed oil by Candida sp. 99-125.
- Published
- 2018