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The Ziziphora tenuior L. is an herbaceous, plant rich in essential oil. Ziziphora tenuior L. has antibacterial and disinfection effect and its vapour is used to treat respiratory tract problems and removing symptoms of the common cold. This plant is carminative, promotes expelling of phlegm and sputum and is a tonic for the stomach. To perform this study some chemical factors (triglyceride, cholesterol, HDL, LDL, ALT, AST and total protein) of the rat's serum were measured in the clinical laboratory. In this study, number of 24 white male wistar rats with the weight range of 215 ± 15 g and at 10 weeks of age were prepared from the Laboratory Animals Breeding Center of the University. Rats were divided into three classes of 8 and 200 mg/kg and 400 mg/kg of the Ziziphora tenuior L. plant essence was used in groups 1 and 2 respectively and no plant compound was used in the third group as the control group. The plant was prescribed for 21 days in groups. Amount of the cholesterol, ALT and triglyceride factors showed the significant difference compared to the control group.