1. Association between global DNA methylation (LINE-1) and occupational particulate matter exposure among informal electronic-waste recyclers in Ghana.
- Author
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Issah, Ibrahim, Arko-Mensah, John, Rozek, Laura S., Rentschler, Katie, Agyekum, Thomas P., Dwumoh, Duah, Batterman, Stuart, Robins, Thomas G., and Fobil, Julius N.
- Subjects
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PARTICULATE matter , *WASTE recycling , *CONFIDENCE intervals , *OCCUPATIONAL exposure , *BLUE collar workers , *REGRESSION analysis , *DNA methylation , *PSYCHOSOCIAL factors , *DESCRIPTIVE statistics - Abstract
This study examined the associations between PM (2.5 and 10) and global DNA methylation among 100 e-waste workers and 51 non-e-waste workers serving as controls. Long interspersed nucleotide repetitive elements-1 (LINE-1) was measured by pyrosequencing. Personal PM2.5 and PM10 were measured over a 4-hour work-shift using real-time particulate monitors incorporated into a backpack. Linear regression models were used to assess the association between PM and LINE-1 DNA methylation. The concentrations of PM2.5 and PM10 were significantly higher among the e-waste workers than the controls (77.32 vs 34.88, p < 0.001 and 210.21 vs 121.92, p < 0.001, respectively). PM2.5 exposure was associated with increased LINE-1 CpG2 DNA methylation (β = 0.003; 95% CI; 0.001, 0.006; p = 0.022) but not with the average of all 4 CpG sites of LINE-1. In summary, high levels of PM2.5 exposure was associated with increased levels of global DNA methylation in a site-specific manner. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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