1. The chromatographic analysis of oligosaccharides and preparation of 1-kestose and nystose in yacon.
- Author
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Zhu, Zhen-Yuan, Lian, Hong-Yu, Si, Chuan-Ling, Liu, Yang, Liu, Nian, Chen, Jing, Ding, Li-Na, Yao, Qiang, and Zhang, Yongmin
- Subjects
OLIGOSACCHARIDES ,YACON ,GLUCOSE ,QUALITATIVE research ,HIGH performance liquid chromatography ,SUCROSE ,SILICA gel ,MASS spectrometry - Abstract
The thin-layer chromatographic analysis of the crude oligosaccharides extracted from yacon revealed the presence of glucose, fructose, sucrose, 1-kestose and nystose. The qualitative and quantitative analysis was carried out on oligosaccharides by high pressure liquid chromatography and the results showed that the contents of d-glucose, fructose, sucrose, 1-kestose, nystose and 1-fructofuranosyl nystose in oligosaccharides were 38.30%, 16.44%, 14.58%, 12.29%, 12.17%, 6.20%, respectively. The content of the fructooligosaccharides in oligosaccharides was 30.66%. The crude oligosaccharides were separated and purified by silica gel column chromatography. The two fractions obtained from crude oligosaccharides were 1-kestose and nystose, which were identified by mass spectra. The yield of 1-kestose and nystose were 10.36% and 9.73%, respectively. The purity of 1-kestose was 82.9% and of nystose was 73.6%. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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