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In this paper, the Sphere-packing bound, Wang-Xing-Safavi-Naini bound, Johnson bound and Gilbert-Varshamov bound on the subspace code of length 2 ν + δ , size M , minimum subspace distance 2 j based on m -dimensional totally singular subspace in the (2 ν + δ) -dimensional orthogonal space 𝔽 q (2 ν + δ) over finite fields 𝔽 q of characteristic 2, denoted by (2 ν + δ , M , 2 j , m) q , are presented, where ν is a positive integer, δ = 0 , 1 , 2 , 0 ≤ m ≤ ν , 0 ≤ j ≤ m. Then, we prove that (2 ν + δ , M , 2 j , m) q codes attain the Wang-Xing-Safavi-Naini bound if and only if they are certain Steiner structures in ℳ (m , 0 , 0 ; 2 ν + δ) , where ℳ (m , 0 , 0 ; 2 ν + δ) denotes the collection of all the m -dimensional totally singular subspaces in the (2 ν + δ) -dimensional orthogonal space 𝔽 q (2 ν + δ) over 𝔽 q of characteristic 2. Finally, Gilbert-Varshamov bound and linear programming bound on the subspace code (2 ν + δ , M , d) q in ℳ (2 ν + δ) are provided, where ℳ (2 ν + δ) denotes the collection of all the totally singular subspaces in the (2 ν + δ) -dimensional orthogonal space 𝔽 q (2 ν + δ) over 𝔽 q of characteristic 2. [ABSTRACT FROM AUTHOR]