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Flexibility of hamstring muscles is very important in sports which can be measured in many tests. The relationship of each test for measuring on the hamstring muscles flexibility has not been verified. The aim of this study was to examine the correlation between the four tests. Ninety-six participants have received four methods of hamstring muscles flexibility tests consist of sit and reach test (SR), stand and reach test (ST), passive knee extension test (PKE), and passive straight leg raise test (PSLR). Pearson productmoment correlation analysis was used to analyze the relationship between the four tests. This study demonstrated a highly significant correlation between SR and ST (r=0.87), a moderate significant correlation between SR and PSLR (r=0.50), a moderate significant correlation between ST and SLR (r=0.46), and a low significant correlation between PKE and SLR (r=0.33). We conclude that SR and ST were a high correlation in the measurement of hamstring muscle flexibility which can be used interchangeably. [ABSTRACT FROM AUTHOR]