1. Anti-inflammatory agents reduce microglial response, demyelinating process and neuronal toxin uptake in a model of encephalopathy produced by Shiga Toxin 2.
- Author
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Pinto A, Berdasco C, Arenas-Mosquera D, Cangelosi A, Geoghegan PA, Nuñez MC, and Goldstein J
- Subjects
- Animals, Brain Diseases microbiology, Dexamethasone administration & dosage, Dexamethasone pharmacology, Escherichia coli Infections microbiology, Etanercept administration & dosage, Etanercept pharmacology, Humans, Lipopolysaccharides toxicity, Male, Mice, Microglia pathology, Oligodendroglia drug effects, Oligodendroglia pathology, Rats, Rats, Sprague-Dawley, Anti-Inflammatory Agents administration & dosage, Brain Diseases drug therapy, Enterohemorrhagic Escherichia coli pathogenicity, Escherichia coli Infections drug therapy, Microglia drug effects, Shiga Toxin 2 toxicity
- Abstract
Infections by Enterohemorrhagic Escherichia coli may cause in addition to hemolytic uremic syndrome neurological disorders which may lead to fatal outcomes in patients. The brain striatum is usually affected during this outcome. The aim of this study was to determine in this area the role of the microglia in pro-inflammatory events that may occur during Shiga toxin 2 intoxication and consequently to this, whether oligodendrocytes were being affected. In the present paper we demonstrated that anti-inflammatory treatments reduced deleterious effects in brain striatal cells exposed to Shiga toxin 2 and LPS. While dexamethasone treatment decreased microglial activation and recovered myelin integrity in the mice striatum, etanercept treatment decreased neuronal uptake of Stx2 in rat striatal neurons, improving the affected area from toxin-derived injury. In conclusion, microglial activation is related to pro-inflammatory events that may deteriorate the brain function during intoxication with Stx2 and LPS. Consequently, the role of anti-inflammatory agents in the treatment of EHEC-derived encephalopathy should be studied in clinical trials., (Copyright © 2018 Elsevier GmbH. All rights reserved.)
- Published
- 2018
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