1. Jacalin-Curcumin Complex Sensitizes the Breast Cancer MDA-MB-231 Cell Line.
- Author
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Petrova, Lidiya, Gergov, Nikolay, Stoup, Marie, Zapryanova, Silvina, Van Damme, Els J. M., Lebègue, Nicolas, Liberelle, Maxime, Zasheva, Diana, and Bogoeva, Vanya
- Subjects
CANCER cells ,TRIPLE-negative breast cancer ,BREAST cancer ,CELL lines ,CELL physiology ,BREAST - Abstract
Protein–drug interactions are crucial for understanding drug delivery and cell functions. Jacalin is a suitable molecule for such targeting, as it specifically recognizes the tumor-associated Thomsen–Friedenreich (TF) antigen that is expressed on the glycosylated proteins in cancer cells. The present paper describes the interaction of curcumin and jacalin, a possible carrier molecule for the delivery of antitumor drugs due to its ability to recognize tumor cells. Our results have shown that both steady-state fluorescence and fluorescent labelling of jacalin are two reliable methods to determine jacalin-curcumin interactions. The affinity of jacalin for curcumin is consistently within the micromolar range (using fluorescence and microscale thermophoresis) showing high-affinity binding of the complex. In vitro experiments on triple-negative breast cancer MDA-MB-231 cells indicated inhibition of cell growth after treating with the jacalin-curcumin complex for 48 h. The cell survival fraction was significantly reduced to 50% after combined treatment. In this paper, we report for the first time about the jacalin-curcumin interaction. We quantified this unique biomolecular interaction and gathered additional information on the binding event. We observed that the jacalin-curcumin complex inhibits the proliferation of the triple-negative breast cancer MDA-MB-231 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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