1. Graphene Oxide Scaffold Stimulates Differentiation and Proangiogenic Activities of Myogenic Progenitor Cells
- Author
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Aleksandra Wędzińska, André Chwalibog, Jaśmina Bałaban, Mateusz Wierzbicki, Malwina Sosnowska, Ewa Sawosz, Marta Kutwin, Sławomir Jaworski, Barbara Wójcik, and Anna Hotowy
- Subjects
0301 basic medicine ,Vascular Endothelial Growth Factor A ,Scaffold ,Angiogenesis ,Cell ,Gene Expression ,02 engineering and technology ,Chick Embryo ,Microscopy, Atomic Force ,Chorioallantoic Membrane ,lcsh:Chemistry ,chemistry.chemical_compound ,angiogenesis ,Cell Movement ,lcsh:QH301-705.5 ,Spectroscopy ,Tissue Scaffolds ,Muscle cell differentiation ,Cell Differentiation ,General Medicine ,021001 nanoscience & nanotechnology ,Computer Science Applications ,Cell biology ,Vascular endothelial growth factor ,medicine.anatomical_structure ,graphene oxide ,Graphite ,Signal transduction ,0210 nano-technology ,myogenic progenitor cells ,Neovascularization, Physiologic ,Catalysis ,Article ,Inorganic Chemistry ,03 medical and health sciences ,medicine ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,Physical and Theoretical Chemistry ,Progenitor cell ,Molecular Biology ,MyoD Protein ,Organic Chemistry ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,030104 developmental biology ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 - Abstract
The physiological process of muscle regeneration is quite limited due to low satellite cell quantity and also the inability to regenerate and reconstruct niche tissue. The purpose of the study was to examine whether a graphene oxide scaffold is able to stimulate myogenic progenitor cell proliferation and the endocrine functions of differentiating cells, and therefore, their active participation in the construction of muscle tissue. Studies were carried out using mesenchymal cells taken from 6-day-old chicken embryos and human umbilical vein endothelial cells (HUVEC) were used to assess angiogenesis. The graphene scaffold was readily colonized by myogenic progenitor cells and the cells dissected from heart, brain, eye, and blood vessels did not avoid the scaffold. The scaffold strongly induced myogenic progenitor cell signaling pathways and simultaneously activated proangiogenic signaling pathways via exocrine vascular endothelial growth factor (VEGF) secretion. The present study revealed that the graphene oxide (GO) scaffold initiates the processes of muscle cell differentiation due to mechanical interaction with myogenic progenitor cell.
- Published
- 2020
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