1. Isolation and Characterization of Polymorphic Microsatellite Loci from Metapenaeopsis barbata Using PCR-Based Isolation of Microsatellite Arrays (PIMA)
- Author
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Ching Ju Cho, Hung Du Lin, Tzen Yuh Chiang, Tzong Der Tzeng, and Feng-Jiau Lin
- Subjects
Conservation genetics ,microsatellite ,China ,Linkage disequilibrium ,Penaeidae ,Taiwan ,RAPD-PCR enrichment ,Locus (genetics) ,Biology ,Polymerase Chain Reaction ,Article ,Catalysis ,law.invention ,lcsh:Chemistry ,Inorganic Chemistry ,law ,Metapenaeopsis barbata ,Genetic variation ,Animals ,Transition Temperature ,Nucleotide Motifs ,Physical and Theoretical Chemistry ,Allele ,lcsh:QH301-705.5 ,Molecular Biology ,Spectroscopy ,Polymerase chain reaction ,Oligonucleotide Array Sequence Analysis ,Repetitive Sequences, Nucleic Acid ,Genetics ,PIMA ,Polymorphism, Genetic ,Organic Chemistry ,General Medicine ,biology.organism_classification ,Computer Science Applications ,lcsh:Biology (General) ,lcsh:QD1-999 ,Genetic Loci ,Microsatellite ,management ,Microsatellite Repeats - Abstract
The red-spot prawn, Metapenaeopsis barbata, is a commercially important, widely distributed demersal species in the Indo-West Pacific Ocean. Overfishing has made its populations decline in the past decade. To study conservation genetics, eight polymorphic microsatellite loci were isolated. Genetic characteristics of the SSR (simple sequence repeat) fingerprints were estimated in 61 individuals from adjacent seas of Taiwan and China. The number of alleles, ranging from 2 to 4, as well as observed and expected heterozygosities in populations, ranging from 0.048 to 0.538, and 0.048 and 0.654, respectively, were detected. No deviation from Hardy–Weinberg expectations was detected at either locus. No significant linkage disequilibrium was detected in locus pairs. The polymorphic microsatellite loci will be useful for investigations of the genetic variation, population structure, and conservation genetics of this species. more...
- Published
- 2012
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