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Your search keyword '"Wang, Weiwei"' showing total 21 results
Start Over Author "Wang, Weiwei" Journal international journal of molecular sciences
21 results on '"Wang, Weiwei"'

1. Reference Gene Selection for RT-qPCR Normalization in Toxoplasma gondii Exposed to Broxaldine.

Author

Qiu, Yanhua, Bai, Yubin, Wang, Weiwei, Wang, Qing, Chen, Shulin, and Zhang, Jiyu

Subjects
GENE expression, POLYMERASE chain reaction, TOXOPLASMA gondii, GENES
Abstract

Reverse transcription–quantitative real-time polymerase chain reaction (RT-qPCR) is widely used to accurately assess target gene expression. Evaluating gene expression requires the selection of appropriate reference genes. To identify reliable reference genes for Toxoplasma gondii (T. gondii) under varying concentrations of broxaldine (BRO), we employed the ΔCt method, BestKeeper, NormFinder, GeNorm, and the comprehensive web-based platform RefFinder to assess the expression stability of ten candidate reference genes in T. gondii. Herein, our findings reveal that the stability of these candidate reference genes is influenced by different experimental conditions. Under normal conditions, the most stable genes were TGME49_205470 and TGME49_226020. However, the most stable genes differed when BRO concentrations were at 1, 2, and 4 μg/mL. Across all samples, TGME49_247220 and TGME49_235930 were identified as the most stable reference genes. Moreover, we also confirmed the stability of TGME49_247220 and TGME49_235930 as reference genes through RT-qPCR assays. The present study provides a foundation for applying the RT-qPCR method to investigate target gene expression following BRO treatment in T. gondii. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim

Author

Qin Lu, Yongzhong Cui, Jinwen Zhang, Wang Weiwei, Nawaz Haider Bashir, Hang Chen, and Haixia Wu

Subjects
0106 biological sciences, 0301 basic medicine, histological structure, distribution pattern, QH301-705.5, Biology, Polysaccharide, 01 natural sciences, Catalysis, Inorganic Chemistry, Transcriptome, 03 medical and health sciences, plant defense, Gene expression, Botany, Plant defense against herbivory, Physical and Theoretical Chemistry, Biology (General), Molecular Biology, QD1-999, Spectroscopy, chemistry.chemical_classification, glandular trichome, Organic Chemistry, General Medicine, Metabolism, Trichome, Computer Science Applications, Staining, gene expression pattern, secretion, Chemistry, 030104 developmental biology, chemistry, Stalk, 010606 plant biology & botany
Abstract

Rhus potaninii Maxim is an economically and medicinally important tree species in China. It produces galls (induced by aphids) with a high abundance of tannins. Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii. A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf. The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8–15 cells. Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids. The dense glandular trichomes provide mechanical protection for young tissues, additionally, their secretion protects the young tissues from pathogens by a special chemical component. According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids. This shows a similar tendency to the staining. The numbers of differentially expressed genes were large or small, the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways. Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense. To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.

Published
2021
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17. Proteomic Analysis of Roots Response to Potassium Deficiency and the Effect of TaHAK1-4A on K + Uptake in Wheat.

Author

Xu, Ke, Zhao, Yong, Yu, Yaxin, Sun, Ruoxi, Wang, Weiwei, Zhang, Shuhua, and Yang, Xueju

Subjects
HYPOKALEMIA, PROTEOMICS, POST-translational modification, IMMOBILIZED proteins, CARBOHYDRATE metabolism
Abstract

Potassium (K+) is essential for plant growth and stress responses. A deficiency in soil K+ contents can result in decreased wheat quality and productivity. Thus, clarifying the molecular mechanism underlying wheat responses to low-K+ (LK) stress is critical. In this study, a tandem mass tag (TMT)-based quantitative proteomic analysis was performed to investigate the differentially abundant proteins (DAPs) in roots of the LK-tolerant wheat cultivar "KN9204" at the seedling stage after exposure to LK stress. A total of 104 DAPs were identified in the LK-treated roots. The DAPs related to carbohydrate and energy metabolism, transport, stress responses and defense, and post-translational modifications under LK conditions were highlighted. We identified a high-affinity potassium transporter (TaHAK1-4A) that was significantly up-regulated after the LK treatment. Additionally, TaHAK1-4A was mainly expressed in roots, and the encoded protein was localized in the plasma membrane. The complementation assay in yeast suggested that TaHAK1-4A mediates K+ uptake under extreme LK conditions. The overexpression of TaHAK1-4A increased the fresh weight and root length of Arabidopsis under LK conditions and improved the growth of Arabidopsis athak5 mutant seedlings, which grow poorly under LK conditions. Moreover, silencing of TaHAK1-4A in wheat roots treated with LK stress decreased the root length, dry weight, K+ concentration, and K+ influx. Accordingly, TaHAK1-4A is important for the uptake of K+ by roots exposed to LK stress. Our results reveal the protein metabolic changes in wheat induced by LK stress. Furthermore, we identified a candidate gene potentially relevant for developing wheat lines with increased K+ use efficiency. [ABSTRACT FROM AUTHOR]

Published
2022
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