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Your search keyword '"floury endosperm"' showing total 2 results
Start Over Descriptor "floury endosperm" Journal international journal of molecular sciences
2 results on '"floury endosperm"'

1. A Novel Mutation of OsPPDKB, Encoding Pyruvate Orthophosphate Dikinase, Affects Metabolism and Structure of Starch in the Rice Endosperm

Author

Long Zhang, Qiaoquan Liu, Linglong Zhao, Lingshang Lin, Cunxu Wei, and Lingxiao Zhao

Subjects
0106 biological sciences, 0301 basic medicine, Starch, Mutant, 01 natural sciences, Catalysis, Endosperm, Inorganic Chemistry, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Amylose, amyloplast development, Physical and Theoretical Chemistry, floury endosperm, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, rice, starch, Organic Chemistry, fungi, food and beverages, General Medicine, Subcellular localization, Computer Science Applications, Complementation, Chloroplast, pyruvate orthophosphate dikinase, 030104 developmental biology, chemistry, Biochemistry, lcsh:Biology (General), lcsh:QD1-999, Cytoplasm, 010606 plant biology & botany
Abstract

Starch, as a main energy storage substance, plays an important role in plant growth and human life. Despite the fact that several enzymes and regulators involved in starch biosynthesis have been identified, the regulating mechanism of starch synthesis is still unclear. In this study, we isolated a rice floury endosperm mutant M14 from a mutant pool induced by 60Co. Both total starch content and amylose content in M14 seeds significantly decreased, and starch thermal and pasting properties changed. Compound starch granules were defected in the floury endosperm of M14 seeds. Map-based cloning and a complementation test showed that the floury endosperm phenotype was determined by a gene of OsPPDKB, which encodes pyruvate orthophosphate dikinase (PPDK, EC 2.7.9.1). Subcellular localization analysis demonstrated that PPDK was localized in chloroplast and cytoplasm, the chOsPPDKB highly expressed in leaf and leaf sheath, and the cyOsPPDKB constitutively expressed with a high expression in developing endosperm. Moreover, the expression of starch synthesis-related genes was also obviously altered in M14 developing endosperm. The above results indicated that PPDK played an important role in starch metabolism and structure in rice endosperm.

Published
2018

2. A Novel Mutation of OsPPDKB, Encoding Pyruvate Orthophosphate Dikinase, Affects Metabolism and Structure of Starch in the Rice Endosperm.

Author

Zhang, Long, Zhao, Linglong, Lin, Lingshang, Zhao, Lingxiao, Liu, Qiaoquan, and Wei, Cunxu

Subjects
*PYRUVATES, *BIOSYNTHESIS, *AMYLOSE, *ENDOSPERM, *CYTOPLASM, *ORTHOPHOSPHATES
Abstract

Starch, as a main energy storage substance, plays an important role in plant growth and human life. Despite the fact that several enzymes and regulators involved in starch biosynthesis have been identified, the regulating mechanism of starch synthesis is still unclear. In this study, we isolated a rice floury endosperm mutant M14 from a mutant pool induced by 60Co. Both total starch content and amylose content in M14 seeds significantly decreased, and starch thermal and pasting properties changed. Compound starch granules were defected in the floury endosperm of M14 seeds. Map-based cloning and a complementation test showed that the floury endosperm phenotype was determined by a gene of OsPPDKB, which encodes pyruvate orthophosphate dikinase (PPDK, EC 2.7.9.1). Subcellular localization analysis demonstrated that PPDK was localized in chloroplast and cytoplasm, the chOsPPDKB highly expressed in leaf and leaf sheath, and the cyOsPPDKB constitutively expressed with a high expression in developing endosperm. Moreover, the expression of starch synthesis-related genes was also obviously altered in M14 developing endosperm. The above results indicated that PPDK played an important role in starch metabolism and structure in rice endosperm. [ABSTRACT FROM AUTHOR]

Published
2018
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