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Your search keyword '"truncated o-glycans"' showing total 2 results
Start Over Descriptor "truncated o-glycans" Journal international journal of molecular sciences
2 results on '"truncated o-glycans"'

1. Mucins and Truncated

Author

Ricardo, Coelho, Lara, Marcos-Silva, Nuno, Mendes, Daniela, Pereira, Catarina, Brito, Francis, Jacob, Catharina, Steentoft, Ulla, Mandel, Henrik, Clausen, Leonor, David, and Sara, Ricardo

Subjects
Glycosylation, endocrine system diseases, MUC16, Transplantation, Heterologous, Mice, Nude, MUC1, Article, Polysaccharides, Cell Line, Tumor, Biomarkers, Tumor, COSMC, Animals, Humans, Ovarian Neoplasms, truncated O-glycans, Gene Expression Profiling, Mucin-1, Membrane Proteins, female genital diseases and pregnancy complications, Cystadenocarcinoma, Serous, Phenotype, CA-125 Antigen, Female, ovarian cancer cell lines, Molecular Chaperones, serous ovarian carcinomas
Abstract

Optimal research results rely on the selection of cellular models capable of recapitulating the characteristics of primary tumours from which they originate. The expression of mucins (MUC16 and MUC1) and truncated O-glycans (Tn, STn and T) represents a characteristic footprint of serous ovarian carcinomas (SOCs). Therefore, selecting ovarian cancer (OVCA) cell lines that reflect this phenotype is crucial to explore the putative biological role of these biomarkers in the SOC setting. Here, we investigated a panel of OVCA cell lines commonly used as SOC models, and tested whether, when cultured in 2D and 3D conditions, these recapitulate the mucin and O-glycan expression profiles of SOCs. We further explored the role of truncating the O-glycosylation capacity in OVCAR3 cells through knockout of the COSMC chaperone, using in vitro and in vivo assays. We found that the majority of OVCA cell lines of serous origin do not share the mucin and truncated O-glycan footprint of SOCs, although 3D cultures showed a higher resemblance. We also found that genetic truncation of the O-glycosylation capacity of OVCAR3 cells did not enhance oncogenic features either in vitro or in vivo. This study underscores the importance of well-characterized cellular models to study specific features of ovarian cancer.

Published
2018

2. Mucins and Truncated O -Glycans Unveil Phenotypic Discrepancies between Serous Ovarian Cancer Cell Lines and Primary Tumours.

Author

Coelho R, Marcos-Silva L, Mendes N, Pereira D, Brito C, Jacob F, Steentoft C, Mandel U, Clausen H, David L, and Ricardo S

Subjects
Animals, Biomarkers, Tumor genetics, Biomarkers, Tumor metabolism, CA-125 Antigen genetics, Cell Line, Tumor, Cystadenocarcinoma, Serous genetics, Cystadenocarcinoma, Serous pathology, Female, Gene Expression Profiling, Glycosylation, Humans, Membrane Proteins genetics, Mice, Nude, Molecular Chaperones genetics, Molecular Chaperones metabolism, Mucin-1 genetics, Ovarian Neoplasms genetics, Ovarian Neoplasms pathology, Phenotype, Transplantation, Heterologous, CA-125 Antigen metabolism, Cystadenocarcinoma, Serous metabolism, Membrane Proteins metabolism, Mucin-1 metabolism, Ovarian Neoplasms metabolism, Polysaccharides metabolism
Abstract

Optimal research results rely on the selection of cellular models capable of recapitulating the characteristics of primary tumours from which they originate. The expression of mucins (MUC16 and MUC1) and truncated O -glycans (Tn, STn and T) represents a characteristic footprint of serous ovarian carcinomas (SOCs). Therefore, selecting ovarian cancer (OVCA) cell lines that reflect this phenotype is crucial to explore the putative biological role of these biomarkers in the SOC setting. Here, we investigated a panel of OVCA cell lines commonly used as SOC models, and tested whether, when cultured in 2D and 3D conditions, these recapitulate the mucin and O -glycan expression profiles of SOCs. We further explored the role of truncating the O -glycosylation capacity in OVCAR3 cells through knockout of the COSMC chaperone, using in vitro and in vivo assays. We found that the majority of OVCA cell lines of serous origin do not share the mucin and truncated O -glycan footprint of SOCs, although 3D cultures showed a higher resemblance. We also found that genetic truncation of the O -glycosylation capacity of OVCAR3 cells did not enhance oncogenic features either in vitro or in vivo. This study underscores the importance of well-characterized cellular models to study specific features of ovarian cancer.

Published
2018
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