1. Elimination of ESAT-6 and CFP-10 from a candidate vaccine against bovine tuberculosis impaired its protection efficacy in the BALBc mouse model
- Author
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Maria Emilia Eirin, Elizabeth Andrea Garcia, Laura Inés Klepp, Fabiana Bigi, Federico Carlos Blanco, and Ximena Ferrara Muñiz
- Subjects
Microbiology (medical) ,Tuberculosis ,030231 tropical medicine ,lcsh:QR1-502 ,complex mixtures ,lcsh:Microbiology ,cfp-10 ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Immune system ,Antigen ,Bacterial Proteins ,vaccine ,medicine ,Animals ,030212 general & internal medicine ,bovine tuberculosis ,esat-6 ,Mycobacterium bovis ,Antigens, Bacterial ,Vaccines ,CFP-10 ,biology ,medicine.disease ,biology.organism_classification ,bacterial infections and mycoses ,Virology ,Diva ,Vaccination ,mycobacterium bovis ,Infectious Diseases ,ESAT-6 ,Cattle ,Tuberculosis, Bovine - Abstract
Background Bovine tuberculosis (bTB) is a zoonotic disease caused by Mycobacterium bovis that mainly affects cattle. Although vaccination is the most effective strategy to control bTB, it may interfere with the diagnosis of the infection. Therefore, ancillary tests to differentiate vaccinated from infected animals (DIVA) are essential in a cattle vaccination scenario. ESAT-6 and CFP-10 are the most promissory DIVA antigens. Method In this study, we deleted esat6 and cfp10 genes from the M. bovis Δ mce2 live-attenuated vaccine candidate and evaluated its protection level against bTB in BALBc mice. Results We found that the M. bovis strain mutant in mce2, esat-6 and cfp-10 failed to confer protection against virulent M. bovis challenge in a mouse model of tuberculosis. Conclusions This result highlights the relevant role of ESAT-6 and CFP-10 in the induction of protective immune response against M. bovis infection and reveals the need of evaluating different strategies to compensate for the lack of these DIVA antigens in new vaccine formulations.
- Published
- 2020