1. Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein
- Author
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Rashmi DarshaKumar Dhurvas, William Viveiros, Adriana K. Cavalcante, Kavita K. Katti, Alice Raphael Karikachery, Sizue Ota Rogero, Jorge G.S. Batista, Gisela de Assis Martini, Kattesh V. Katti, Kelme C. Damasceno, Velaphi C. Thipe, Jose R. Rogero, Ademar B. Lugão, Matheus B. Ormenio, Mohanavelu Nappinnai, DarshaKumar Dhurvas Mohandoss, and Joana da S. Maziero
- Subjects
food.ingredient ,Chemistry ,Organic Chemistry ,Biophysics ,Pharmaceutical Science ,Bioengineering ,02 engineering and technology ,General Medicine ,Pharmacology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Silver nanoparticle ,In vitro ,0104 chemical sciences ,Biomaterials ,food ,In vivo ,Drug Discovery ,Toxicity ,Gum arabic ,Ecotoxicity ,0210 nano-technology ,Cytotoxicity ,EC50 - Abstract
Introduction We report, herein, in vitro, and in vivo toxicity evaluation of silver nanoparticles stabilized with gum arabic protein (AgNP-GP) in Daphnia similis, Danio rerio embryos and in Sprague Dawley rats. Purpose The objective of this investigation was to evaluate in vitro and in vivo toxicity of silver nanoparticles stabilized with gum arabic protein (AgNP-GP), in multispecies due to the recognition that toxicity evaluations beyond a single species reflect the environmental realism. In the present study, AgNP-GP was synthesized through the reduction of silver salt using the tri-alanine-phosphine peptide (commonly referred to as "Katti Peptide") and stabilized using gum arabic protein. Methods In vitro cytotoxicity tests were performed according to ISO 10993-5 protocols to assess cytotoxicity index (IC50) values. Acute ecotoxicity (EC50) studies were performed using Daphnia similis, according to the ABNT NBR 15088 protocols. In vivo toxicity also included evaluation of acute embryotoxicity using Danio rerio (zebrafish) embryos following the OECD No. 236 guidelines. We also used Sprague Dawley rats to assess the toxicity of AgNP-GP in doses from 2.5 to 10.0 mg kg-1 body weight. Results AgNP-GP nanoparticles were characterized through UV (405 nm), core size (20±5 nm through TEM), hydrodynamic size (70-80 nm), Zeta (ζ) potential (- 26 mV) using DLS and Powder X ray diffraction (PXRD) and EDS. PXRD showed pattern consistent with the Ag (1 1 1) peak. EC50 in Daphnia similis was 4.40 (3.59-5.40) μg L-1. In the zebrafish species, LC50 was 177 μg L-1. Oral administration of AgNP-GP in Sprague Dawley rats for a period of 28 days revealed no adverse effects in doses of up to 10.0 mg kg-1 b.w. in both male and female animals. Conclusion The non-toxicity of AgNP-GP in rats offers a myriad of applications of AgNP-GP in health and hygiene for use as antibiotics, antimicrobial and antifungal agents.
- Published
- 2020