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The aim of the present study is to reveal the possible mechanism of anti-inflammatory activity of Crateva adansonii leaf extract to claim folkoric use of the plant in inflammation disease conditions. Isolation of chloroform leaf extract fraction (CEF) containing anti-inflammatory marker compound of the plant lupeol is done through column chromatography procedure, which is further confirmed by HPLC analysis with standard lupeol compound. The isolated marker compound lupeol from CEF shows significant in vitro anti-inflammatory effect compared to methanolic and chloroform leaf extracts (ME & CE) of the plant Crateva adansonii and reference standard indomethacin. Further in silico screening of lupeol and indomethacin against five crucial inflammatory molecular targets such as COX-2 (PDB ID: 4COX), MPO (PDB ID: 3ZS0), IL1β (PDBID: 1T4Q), IL6 (PDBID: 19PM) and TNFα (PDBID: 2AZ5) was done using autodock tool. Maximal binding affinity (− 11.6, − 9.0, − 9.9, − 7.5, − 9.0 kcal/mol) was exhibited by lupeol against all five targets of inflammation COX-2, MPO, TNFα, IL1β & IL6 respectively. In vitro and in silico modeling confirms the anti-inflammatory efficiency of lupeol and also unveils the hidden molecular mechanisms of folkoric use of Crateva adansonii plant in inflammatory disease conditions. [ABSTRACT FROM AUTHOR]

The decolourization of the azo dye (acid yellow) by laccase from Bacillus sp. strain TR under submerged fermentation (SmF) was optimized by response surface methodology (RSM). The laccase maximum yield was achieved at 96 h of SmF with pH 7.0, 1.0 g/L of maltose and 3.0 g/L of ammonium acetate at 37 °C. The enzyme yield was estimated that 570 U/mL. About 76.4% of acid yellow decolourization efficiency was observed by the laccase enzyme within 96 h. The substrate surface changes were observed before and after the laccase treatment was analyzed with the scanning electron microscope (SEM) and N = N transformation either nitrogen or ammonia was showed by Fourier transform infrared spectroscopy (FT-IR) analysis. The HPLC analysis explained the formation of various intermediates in the conversion of acid yellow to final products. Further, in silico studies proved the enzyme–substrate interactions and showed a better score of − 27.435 kJ/mol. [ABSTRACT FROM AUTHOR]

Marine snails are abundant sources of biologically important conopeptides with their potential applications in drug development. The present study aimed to identify the potential conopeptides from the venom duct of Conus inscriptus. After extraction conopeptides were characterized by liquid chromatography–mass spectrometric analysis showing totally 29 protein sequences with disulfide linkages of different molecular mass distributed in the range of 387–1536 m/z and the peptides showing mostly to T-superfamily of conotoxins with 78 kDa heat shock proteins. The venom peptides showed six different molecular weight bands (37, 51, 60, 70, 80 and 90 kDa) above 30 kDa after in-gel enzymatic digestion. Furthermore, the conopeptides exhibit potential cytotoxic activity against HeLa-HPV 16 associated, Vero (normal) cell lines and brine shrimp. Fourier transform infra-red spectroscopy analysis confirms the structural and functional groups. The observed results suggests the venom peptides from C. inscriptus as a potential anticancer agent. [ABSTRACT FROM AUTHOR]