1. Streptomyces sedi sp. nov., isolated from surface-sterilized roots of Sedum sp.
- Author
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Li J, Zhao GZ, Qin S, Zhu WY, Xu LH, and Li WJ
- Subjects
- Bacterial Typing Techniques, Base Composition, China, DNA Gyrase genetics, DNA, Bacterial analysis, DNA, Ribosomal analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Polymerase Chain Reaction methods, Quinones analysis, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Species Specificity, Streptomyces genetics, Streptomyces isolation & purification, Streptomyces physiology, Plant Roots microbiology, Sedum microbiology, Streptomyces classification
- Abstract
An endophytic actinomycete, strain YIM 65188(T), was isolated from surface-sterilized tissue of Sedum sp. collected from Yunnan province, south-west China, and characterized by using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain YIM 65188(T) is a member of the genus Streptomyces and exhibited 97.5 and 96.3 % gene sequence similarities to Streptomyces specialis GW41-1564(T) and Streptomyces hainanensis YIM 47672(T), respectively, whereas low sequence similarity values (<95.2 %) distinguished strain YIM 65188(T) from all other Streptomyces species with validly published names. Strain YIM 65188(T) had a unique menaquinone composition, with the predominant quinones being MK-11(H(6)) and MK-10(H(6)), with moderate amounts of MK-10(H(8)), MK-11(H(8)), MK-9(H(8)), MK-11(H(4)), MK-9(H(6)) and MK-10(H(4)). Similarly, the closest phylogenetic relative, S. specialis GW41-1564(T), also had an unusual quinone composition, with the predominant menaquinones MK-10(H(4)) and MK-10(H(6)) and minor amounts of MK-9(H(4)) and MK-9(H(6)). The DNA-DNA hybridization value between strain YIM 65188(T) and S. specialis GW41-1564(T) was 42.7 %. On the basis of phenotypic and phylogenetic characteristics, strain YIM 65188(T) was identified as a novel species of the genus Streptomyces for which the name Streptomyces sedi sp. nov. is proposed, with YIM 65188(T) (=CCTCC AA 208020(T) =DSM 41942(T)) as the type strain.
- Published
- 2009
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