1. Synaptosomal Actin Dynamics in the Developmental Visual Cortex Regulate Behavioral Visual Acuity in Rats.
- Author
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Bi AL, Zhang YY, Lu ZY, Tang HY, Zhang XY, Zhang ZH, Li BQ, Guo DD, Gong S, Li Q, Wang XR, Lu XZ, and Bi HS
- Subjects
- Amblyopia metabolism, Amblyopia physiopathology, Animals, Antineoplastic Agents pharmacology, Behavior, Animal physiology, Depsipeptides pharmacology, Maze Learning, Polymerization drug effects, Rats, Vision, Ocular physiology, Actins chemistry, Actins metabolism, Neuronal Plasticity physiology, Synaptosomes metabolism, Visual Acuity physiology, Visual Cortex physiology
- Abstract
Purpose: Synaptosomal actin dynamics are essential for synaptic structural stability. Whether actin dynamics are involved in structural and functional synaptic plasticity within the primary visual cortex (V1) or behavioral visual acuity in rats has still not been thoroughly investigated., Methods: Synaptosome preparation and western blot analysis were used to analyze synaptosomal actin dynamics. Transmission electron microscopy was used to detect synaptic density and mitochondrial area alterations. A visual water maze task was applied to assess behavioral visual acuity. Microinjection of the actin polymerization inhibitor or stabilizer detected the effect of actin dynamics on visual function., Results: Actin dynamics, the mitochondrial area, and synaptic density within the area of V1 are increased during the critical period for the development of binocularity. Microinjection of the actin polymerization inhibitor cytochalasin D into the V1 decreased the mitochondrial area, synaptic density, and behavioral visual acuity. Long-term monocular deprivation reduced actin dynamics, the mitochondrial area, and synaptic density within the V1 contralateral to the deprived eye compared with those ipsilateral to the deprived eye and impaired visual acuity in the amblyopic eye. In addition, the mitochondrial area, synaptic density, and behavioral visual acuity were improved by stabilization of actin polymerization by jasplakinolide microinjection., Conclusions: During the critical period of visual development of binocularity, synaptosomal actin dynamics regulate synaptic structure and function and play roles in behavioral visual acuity in rats.
- Published
- 2021
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