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The article presents experimental data on the influence of the feeding diet on the biochemical blood parameters of laying hens. As feed additives in the poultry diet were used: feed vitamin-mineral concentrate (FVMC) in the amount of 0.3% of the basic diet (BD) (1st experimental group), probiotic additive “Bacell-M” in the amount of 0.2% of BD (2nd experimental group), complex feed concentrate (CFC), containing FVMC and probiotic additive “Bacell-M”, in the amount of 0.3% of BD (3rd experimental group). The control group received only BD. It was found that the introduction of the feed additives into the diet of laying hens stimulated the metabolic processes in the poultry body, which was manifested by an increase in a number of biochemical constants. So, the level of total protein in the 1st experimental group increased by 34%, in the 2nd and 3rd experimental groups it increased in 1.43 and 1.42 times in comparison with the control group. The urea concentration exceeded the analogical values of the control poultry by 27% (1st experimental group), by 23.2% (2nd experimental group) and by 24.3% (3rd experimental group), respectively. The use of feed additives influenced the state of carbohydrate and lipid metabolism of hens, which was manifested by an increase in glucose concentration in the groups by 6.3%, 13.1% and 21.5% and by an increase of triglycerides by 13.2%, in 1.7 and in 2.0 times respectively. The inclusion of additives in the feeding schemes contributed to the correction of mineral metabolism, providing more complete absorption of calcium by the poultry, the increase of which in the experimental groups was by 16.2%, 1.68% and in 2.21 times, and also prevented the development of hepatocyte cytolysis, as indicated by a decrease in the activity of hepatoindicatory of enzymes: ALT decreased by 9.8%, 29.5% and in 1.85 times, AST decreased by 7.9%, 12.7% and 18.9%, respectively. The maximum positive effect in the indicators of the biochemical status of laying hens was observed when CFC was used in the diet, which was due to the synergy of FVMC and the Bacell-M probiotic additive.