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As previously work has been done on the isolation of free living nitrogen fixers from tea rhizosphere of South Assam, this edition was an additional work for verifying the nitrogen fixing ability of an isolate was an attempt to understand the nitrogenase activity of the isolate. In this paper an isolate has been isolated in a nitrogen free medium, it's morphological and biochemical assessments have been made earlier. And here its nitrogen fixation ability was depicted after doing Acetylene reduction Assay. From that a clear idea of its nitrogen fixing ability has been drawn, even if the capacity is low.