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Introduction: The aim of this study was to investigate this hypothesis that oxytocin can decrease apoptotic index in testis under acute ischemia reperfusion in a rat model. Materials and Methods: Twenty adult rats were randomly divided into 4 groups: 1: eontrols 2: Ischemia- Reperfusion (IR) 3: oxytocin (OT) 4: oxytocin after induction of IR (OTA). Testicular ischemia was achieved by torsion of the left testis 720 clockwise for 2 hours. Two hours after isehemia, torsion was removed and reperftision was performed. Immediately after induction of reperfusion 0.03 μg/kg oxytocin were administered intraperitoneally to the OTA group. Three hours after surgery left testis were removed and evaluations were made by Jhonson's score, Radioimmunoassay (RIA), immunohistochemistry and histomorphometry for study of maturity of spermatogenesis, endocrine profiles, apoptosis and quantitative studies, respectively. Results: Induction of ischemia-reperftision in group2 induced marked degeneration of germ eells, edema and blood vessels congestion in testis. A significant reduced Jhonson's score were detected in IR group in compare with eontrols (p<0.05).and apoptotic index increase significantly (p<0.05) in compare with the controls. Sex hormones were not changed in all experimental groups. Administration of OT in OTA group decrease the apoptotic index in compare with the IR group significantly (p<0.05) and histological parameters same as germinal epithelium thickness and gem cells number were increased but not significant statically. Conclusion: These results suggested that oxytocin can decrease apoptotie index and improves complication of acute isehemic reperfusion in testis in a rat model. [ABSTRACT FROM AUTHOR]

Unlabelled: Ischemia reperfusion (IR) is the main pathology of torsion of testis and it is a common urologic emergency. There is some evidence that shows oxytocin (OT) plays role in ischemia reperfusion., Objective: To evaluate this hypothesis that OT can decrease germ cell apoptotic index in testis under acute ischemia reperfusion in a rat model., Materials and Methods: 20 adult rats were randomly divided into four groups: Control, IR, OT and IR+ OT (OTA). Testicular ischemia was achieved by 720° torsion of the left testis for 2 hr. Then, torsion was removed and reperfusion was performed. Immediately after induction of reperfusion 0.03 µg/kg OT were administered intraperitoneally to the IR+ OT. Three hours after surgery left testis was removed and evaluations were made by Johnson's score, ELISA, immunohistochemistry and histomorphometry for study of maturity of spermatogenesis, endocrine profiles, apoptosis and quantitative studies, respectively., Results: The results showed in addition tissue edema and congestion, a significant reduced in Johnson's score were detected in IR group in comparison with controls (p=0.01), and apoptotic index increased significantly (p=0.001). Administration of OT in OT+IR group, increased Johnson's score but it was not statistically significant. Germinal epithelium thickness was increased significantly (p=0.03), although apoptotic index decreased significantly in comparison with the IR group (p=0.04). However there was not significant difference in serum levels of testosterone, FSH and LH in none of groups (p=0.07)., Conclusion: These results suggested that OT can decrease apoptotic index and improves complication of acute ischemic reperfusion in testis in a rat model.