1. Human islet mass, morphology, and survival after cryopreservation using the Edmonton protocol
- Author
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Miranda, Priya M, Mohan, Viswanathan, Ganthimathy, Sekhar, Anjana, Ranjit M, Gunasekaran, S, Thiagarajan, Venkatachalam, Churchill, Thomas A, Kin, Tatsuya, Shapiro, AM James, and Lakey, Jonathan RT
- Subjects
Diabetes ,Transplantation ,Autoimmune Disease ,Apoptosis ,Cell Survival ,Cryopreservation ,Glucagon ,Humans ,Insulin ,Islets of Langerhans ,Mitotic Index ,Necrosis ,Somatostatin ,Tissue Culture Techniques ,islet transplantation ,cryopreservation ,post-thaw culture ,islet isolation ,clinical phase ,pre-clinical phase ,ultrastructure TUNEL ,immunohistochemistry ,Medical Microbiology ,Medical Physiology - Abstract
The aim of this study was to assess recovery, cell death, and cell composition of post-thaw cultured human islets. Cryopreserved islets were provided by the Clinical Islet Transplant Program, Edmonton, Canada. Islets were processed using media prepared in accordance with Pre-Edmonton and Edmonton protocols. Cryopreserved islets were rapidly thawed and cultured for 24 h, 3 d, 5 d, and 7 d, following which they were processed for histology. Islet quantification, integrity, morphology and tissue turnover were studied via hematoxylin and eosin stained sections. Ultrastructure was studied by electron microscopy and endocrine cell composition by immunohistochemistry. Using the Pre-Edmonton protocol, islet recovery was 50.1% and islet survival was 50% at 24 h while for the Edmonton protocol, the islet recovery was 69.4% (p
- Published
- 2013