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Your search keyword '"Del Sambro, Laura"' showing total 3 results
Start Over Author "Del Sambro, Laura" Journal italian journal of food safety
3 results on '"Del Sambro, Laura"'

1. Isolation of Yersinia pseudotuberculosis in bovine mastitis: A potential milk-borne hazard

Author

Lorusso, Andrea, Addante, Luciana, Capozzi, Loredana, Bianco, Angelica, Del Sambro, Laura, Gallitelli, Maria Ester, and Parisi, Antonio

Subjects
whole genome sequencing, lcsh:Food processing and manufacture, lcsh:TP368-456, Yersinia pseudotuberculosis, milk-borne diseases, mastitis, Case Report
Abstract

This paper describes the first confirmed case of a subclinical mastitis caused by Yersinia pseudotuberculosis in a dairy cow from Italy. Milk samples from an adult cow of the Bruna breed were analyzed accordingly to standard milk cultivation protocols. Bacteriological examinations allowed to isolate atypical Gram-negative rods identified as Y. pseudotuberculosis using biochemical tests. The isolate was subjected to Whole Genome Sequencing (WGS) and the species identification was confirmed using rMLST. Moreover, the virulence and antibacterial susceptibility of the isolate have been also determined. The most common virulence genes were screened through WGS, showing the presence of inv, ail, pil and HPI genes. No antibiotic resistance was found. Even though scarcely described as causal agent of subclinical mastitis, the detection of Y. pseudotubercolusosis suggests that this pathogen could be spread to humans through raw milk, representing a potential food safety hazard.

Published
2021

3. Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products.

Author

Bianco, Angelica, Capozzi, Loredana, Miccolupo, Angela, Iannetti, Simona, Danzetta, Maria Luisa, Del Sambro, Laura, Caruso, Marta, Santagada, Gianfranco, and Parisi, Antonio

Subjects
BACILLUS cereus, DAIRY products, FOOD poisoning, GENES, PUBLIC safety
Abstract

Members of Bacillus cereus group are important food contaminants and they are of relevant interest in food safety and public heath due to their ability to cause two distinct forms of food poisoning, emetic and diarrhoeal syndrome. In the present study, 90 strains of B. cereus isolated from dairy products, have been typed using Multilocus Sequence Typing (MLST) analysis and investigated for the occurrence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, entS and bceT) and one emetogenic gene (ces), to determine their genetic diversity. A total of 58 sequence types were identified and among these 17 were signalled as new profiles. Among the virulence genes, the majority of our strains carried the entS (92%), entFM (86%), nhe (82%) and cytK (72%) genes. All remaining genes were identified in at least one strain with different prevalence, stressing the genetic diversity, how even the different grade of pathogenicity of B. cereus isolated from dairy products. [ABSTRACT FROM AUTHOR]

Published
2020
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