1. Effectiveness of essential oils against dual-species biofilm of Listeria monocytogenes and Pseudomonas fluorescens in a Ricotta-based model system
- Author
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Francesca Maggio, Annalisa Serio, Chiara Rossi, Chiara Purgatorio, Francesco Buccioni, Clemencia Chaves- López, and Antonello Paparella
- Subjects
Thymbra capitata (L.) Cav. ,Cinnamomum zeylanicum ,Listeria monocytogenes ,Pseudomonas fluorescens ,Dairy facilities ,Food processing and manufacture ,TP368-456 - Abstract
Biofilms represent an evolutionary form of life, which translates from life in free-living cells to a community lifestyle. In natural habitats, biofilms are a multispecies complex, where synergies or antagonisms can be established. For example, Listeria monocytogenes and Pseudomonas fluorescens are associated with a dual-species biofilm that is widespread in dairy plants. In food plants, multiple strategies are devised to control biofilms, including natural compounds such as essential oils (EOs). In this respect, this study evaluated the effectiveness of Thymbra capitata (L.) Cav. EO and Cinnamomum zeylanicum (CEO) against a dual-species biofilm of L. monocytogenes and P. fluorescens, mimicking dairy process conditions. Based on Minimum Inhibitory Concentrations results, the EOs concentration (10 µL/mL) was chosen for the antibiofilm assay at 12°C on polystyrene (PS), and stainless steel surfaces for 168 h, using a Ricotta-based model system as culture medium. Biofilm biomass was assessed by crystal violet staining, and the planktonic and sessile cells were quantified in terms of Log CFU/cm2. Results showed that CEO displayed the greatest antibiofilm activity, reducing significantly (P
- Published
- 2023
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