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Normal human adult lymphocytes were cultured with IFNs (α, β, and γ) in different concentrations and at culture times. The cells', supernatants' beta 2-m were measured by enzyme immunoassay. It was found the both beta 2-m were increased dependent upon in the culture IFN doses. The increasing percentages of beta 2-m was different for each IFN. The maximal expression of the cells' beta 2-m was observed for 24-48 hours after IFN treatment, in the IFN, maximal expression of cells' beta 2-m was for 24 hours. In the case of IFN-γ-cells' beta 2-m, they increased higher in the period of 96-hour culture. The increasing rate of beta 2-m with IFN-γ was higher than the IFN-α, β. It is supposed that beta 2-m is a useful new marker against cells' active condition.