1. The Effects of Melatonin in Glutamate-Induced Neurotoxicity of Rat Cerebellar Granular Cell Culture
- Author
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Ahmet Hacimuftuoglu, Selma Düzenli, Halis Suleyman, Dilek Bulucu, and Akçahan Gepdiremen
- Subjects
medicine.medical_specialty ,Programmed cell death ,Glutamic Acid ,Biology ,Neuroprotection ,Antioxidants ,Rats, Sprague-Dawley ,Melatonin ,Granular cell ,Cerebellum ,Internal medicine ,medicine ,Animals ,Dead cell ,Neurons ,Pharmacology ,Cell Death ,Neurotoxicity ,Glutamate receptor ,medicine.disease ,Rats ,Endocrinology ,Animals, Newborn ,Biochemistry ,medicine.drug - Abstract
In the present study, melatonin was tested in subsequent doses in glutamate induced neurotoxicity in cerebellar granular cell culture of rat pups. Glutamate at 10(-7) M was found to induce neuronal cell death. The dead cell score was 2.75+/-0.7 in the control, while it was found to be 35.12+/-1.8 in the glutamate-administered group (P0.0001). Melatonin very potently blocked glutamate neurotoxicity at all doses tested, with 10(-3) M, the highest dose tested, being the most effective. Glutamate may exert a neuroprotective effect by blocking one or more steps of the oxidation cascade in neurons and this effect may be blocked by melatonin.
- Published
- 2000
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