15 results on '"De Prado, R."'
Search Results
2. Morphological and Molecular Characterization of Different Echinochloa spp. and Oryza sativa Populations
- Author
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Ruiz-Santaella, J. P., primary, Bastida, F., additional, Franco, A. R., additional, and De Prado, R., additional
- Published
- 2006
- Full Text
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Catalog
3. Reduced Glyphosate Movement and Mutation of the EPSPS Gene (Pro106Ser) Endow Resistance in Conyza canadensis Harvested in Mexico.
- Author
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Palma-Bautista C, Vázquez-Garcia JG, López-Valencia G, Domínguez-Valenzuela JA, Barro F, and De Prado R
- Subjects
- Mexico, Herbicide Resistance genetics, Mutation, 3-Phosphoshikimate 1-Carboxyvinyltransferase genetics, Glyphosate, Conyza genetics, Herbicides pharmacology
- Abstract
Glyphosate has been the most widely used herbicide for decades providing a unique tool, alone or in mixtures, to control weeds on citrus in Veracruz. Conyza canadensis has developed glyphosate resistance for the first time in Mexico. The level and mechanisms of resistance of four resistant populations Rs (R1, R2, R3, and R4) were studied and compared with that of a susceptible population ( S ). Resistance factor levels showed two moderately resistant populations (R2 and R3) and two highly resistant populations (R1 and R4). Glyphosate translocation through leaves to roots was ∼2.8 times higher in the S population than in the four R populations. A mutation (Pro106Ser) in the EPSPS 2 gene was identified in the R1 and R4 populations. Mutation in the target site associated with reduced translocation is involved in increased glyphosate resistance in the R1 and R4 populations; whereas for the R2 and R3 populations, it was only mediated by reduced translocation. This is the first study of glyphosate resistance in C. canadensis from Mexico in which the resistance mechanisms involved are described in detail and control alternatives are proposed. more...
- Published
- 2023
- Full Text
- View/download PDF
4. Non-Target-Site Resistance Mechanisms Endow Multiple Herbicide Resistance to Five Mechanisms of Action in Conyza bonariensis .
- Author
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Palma-Bautista C, Vázquez-García JG, Domínguez-Valenzuela JA, Ferreira Mendes K, Alcántara de la Cruz R, Torra J, and De Prado R
- Subjects
- 3-Phosphoshikimate 1-Carboxyvinyltransferase, Herbicide Resistance, Acetolactate Synthase, Conyza, Herbicides pharmacology
- Abstract
The repeated use of herbicides can lead to the selection of multiple resistance weeds. Some populations of Conyza bonariensis occurring in olive groves from southern Spain have developed resistance to various herbicides. This study determined the resistance levels to 2,4-D, glyphosate, diflufenican, paraquat, and tribenuron-methyl in a putative resistant (R) C. bonariensis population, and the possible non-target-site resistance (NTSR) mechanisms involved were characterized. Resistance factors varied as follows: glyphosate (8.9), 2,4-D (4.8), diflufenican (5.0), tribenuron-methyl (19.6), and paraquat (85.5). Absorption of
14 C-glyphosate was up to 25% higher in the susceptible (S) population compared to the R one, but14 C-paraquat absorption was similar (up to 70%) in both populations. S plants translocated more than 60% of both14 C-glyphosate and14 C-paraquat toward shoots and roots, while R plants translocated less than 10%. The R population was able to metabolize 57% of the 2,4-D into nontoxic metabolites and 68% of the tribenuron-methyl into metsulfuron-methyl (10%), metsulfuron-methyl-hydroxylate (18%), and conjugate-metsulfuron-methyl (40%). Among the NTSR mechanisms investigated, absorption and translocation could be involved in glyphosate resistance, but only translocation for paraquat. Proofs of the presence of enhanced metabolism as a resistance mechanism were found for tribenuron-methyl and 2,4-D, but not for diflufenican. This research informs the first occurrence of multiple resistance to five herbicide classes (acetolactate synthase inhibitors, 5-enolpyruvylshikimate-3-phosphate synthase inhibitors, photosystem I electron diverters, photosystem II inhibitors, and synthetic auxin herbicides) in C. bonariensis . more...- Published
- 2021
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5. Multiple Herbicide Resistance Evolution: The Case of Eleusine indica in Brazil.
- Author
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Vázquez-García JG, Alcántara-de la Cruz R, Rojano-Delgado AM, Palma-Bautista C, de Portugal Vasconcelos JM, and De Prado R
- Subjects
- Acetyl-CoA Carboxylase antagonists & inhibitors, Acetyl-CoA Carboxylase genetics, Acetyl-CoA Carboxylase metabolism, Brazil, Eleusine enzymology, Eleusine genetics, Enzyme Inhibitors pharmacology, Glycine pharmacology, Imidazoles pharmacology, Paraquat pharmacology, Plant Proteins antagonists & inhibitors, Plant Proteins genetics, Plant Proteins metabolism, Glyphosate, Eleusine drug effects, Glycine analogs & derivatives, Herbicide Resistance, Herbicides pharmacology
- Abstract
The occurrence of multiple herbicide resistant weeds has increased considerably in glyphosate-resistant soybean fields in Brazil; however, the mechanisms governing this resistance have not been studied. In its study, the target-site and nontarget-site mechanisms were characterized in an Eleusine indica population (R-15) with multiple resistance to the acetyl-CoA carboxylase (ACCase) inhibitors, glyphosate, imazamox, and paraquat. Absorption and translocation rates of
14 C-diclofop-methyl14 C-imazamox and14 C-glyphosate of the R-15 population were similar to those of a susceptible (S-15) population; however, the R-15 population translocated ∼38% less14 C-paraquat to the rest of plant and roots than the S-15 population. Furthermore, the R-15 plants metabolized (by P450 cytochrome) 55% and 88% more diclofop-methyl (conjugate) and imazamox (imazamox-OH and conjugate), respectively, than the S-15 plants. In addition, the Pro-106-Ser mutation was found in the EPSPS gene of this population. This report describes the first characterization of the resistance mechanisms in a multiple herbicide resistant weed from Brazil. more...- Published
- 2021
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6. Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards.
- Author
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Mora AD, Rosario J, Rojano-Delgado AM, Palma-Bautista C, Torra J, Alcántara-de la Cruz R, and De Prado R
- Subjects
- 2,4-Dichlorophenoxyacetic Acid metabolism, 2,4-Dichlorophenoxyacetic Acid pharmacology, Asteraceae metabolism, Dicamba metabolism, Dicamba pharmacology, Glycine metabolism, Glycine pharmacology, Herbicides metabolism, Indoleacetic Acids metabolism, Glyphosate, Asteraceae drug effects, Citrus growth & development, Glycine analogs & derivatives, Herbicide Resistance, Herbicides pharmacology, Indoleacetic Acids pharmacology
- Abstract
Dominican farmers have started to apply synthetic auxin herbicides (SAHs) as the main alternative to mitigate the impacts of the occurrence of glyphosate-resistant (GR) Parthenium hysterophorus populations in citrus orchards. A GR P. hysterophorus population survived field labeled rates of glyphosate, 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba, and picloram, which showed poor control (<50%). In in vivo assays, resistance levels were high for glyphosate and moderate for picloram, dicamba, and 2,4-D. Sequencing the 5-enolpyruvylshikimate-3-phosphate synthase gene revealed the double Thr-102-Ile and Pro-106-Ser amino acid substitution, conferring resistance to glyphosate. Additionally, reduced absorption and impaired translocation contributed to this resistance. Regarding SAH, impaired 2,4-D transport and enhanced metabolism were confirmed in resistant plants. The application of malathion improved the efficacy of SAHs (control >50%), showing that metabolism of these herbicides was mediated by cytochrome P450 enzymes. This study reports, for the first time, multiple resistance to SAHs and glyphosate in P. hysterophorus . more...
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- 2019
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7. Reduced Absorption and Impaired Translocation Endows Glyphosate Resistance in Amaranthus palmeri Harvested in Glyphosate-Resistant Soybean from Argentina.
- Author
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Palma-Bautista C, Torra J, Garcia MJ, Bracamonte E, Rojano-Delgado AM, Alcántara-de la Cruz R, and De Prado R
- Subjects
- 3-Phosphoshikimate 1-Carboxyvinyltransferase genetics, 3-Phosphoshikimate 1-Carboxyvinyltransferase metabolism, Amaranthus drug effects, Argentina, Biological Transport, Glycine metabolism, Glycine pharmacology, Herbicide Resistance, Herbicides pharmacology, Plant Leaves drug effects, Plant Leaves metabolism, Plant Proteins genetics, Plant Proteins metabolism, Plant Weeds drug effects, Glycine max growth & development, Glyphosate, Amaranthus metabolism, Glycine analogs & derivatives, Herbicides metabolism, Plant Weeds metabolism, Glycine max drug effects
- Abstract
Amaranthus palmeri S. Watson is probably the worst glyphosate-resistant (GR) weed worldwide. The EPSPS (5-enolpyruvylshikimate-3-phosphate-synthase) gene amplification has been reported as the major target-site-resistance (TSR) mechanism conferring resistance to glyphosate in this species. In this study, TSR and non-target-site-resistance (NTSR) mechanisms to glyphosate were characterized in a putative resistant A. palmeri population (GRP), harvested in a GR soybean crop from Argentina. Glyphosate resistance was confirmed for the GRP population by dose-response assays. No evidence of TSR mechanisms, as well as glyphosate metabolism, was found in this population. Moreover, a susceptible population (GSP) that absorbed about 10% more herbicide than the GRP population was evaluated at different periods after treatment. The GSP population translocated about 20% more glyphosate to the remainder of the shoots and roots at 96 h after treatment than the control, while the GRP population retained 62% of herbicide in the treated leaves. This is the first case of glyphosate resistance in A. palmeri involving exclusively NTSR mechanisms. more...
- Published
- 2019
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8. Correction to Pool of Resistance Mechanisms to Glyphosate in Digitaria insularis.
- Author
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de Carvalho LB, Alves PLDCA, González-Torralva F, Cruz-Hipolito HE, Rojano-Delgado AM, De Prado R, Gil-Humanes J, Barro F, and Luque de Castro MD
- Published
- 2017
- Full Text
- View/download PDF
9. Pool of resistance mechanisms to glyphosate in Digitaria insularis.
- Author
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de Carvalho LB, Alves PL, González-Torralva F, Cruz-Hipolito HE, Rojano-Delgado AM, De Prado R, Gil-Humanes J, Barro F, and de Castro MD
- Subjects
- 3-Phosphoshikimate 1-Carboxyvinyltransferase genetics, 3-Phosphoshikimate 1-Carboxyvinyltransferase metabolism, Brazil, Glycine pharmacokinetics, Glycine pharmacology, Glyoxylates metabolism, Herbicides pharmacology, Isoxazoles, Mutation, Organophosphonates metabolism, Plant Leaves drug effects, Plant Leaves metabolism, Sarcosine metabolism, Shikimic Acid analysis, Shikimic Acid metabolism, Tetrazoles, Glyphosate, Digitaria drug effects, Digitaria physiology, Glycine analogs & derivatives, Herbicide Resistance
- Abstract
Digitaria insularis biotypes resistant to glyphosate have been detected in Brazil. Studies were carried out in controlled conditions to determine the role of absorption, translocation, metabolism, and gene mutation as mechanisms of glyphosate resistance in D. insularis. The susceptible biotype absorbed at least 12% more (14)C-glyphosate up to 48 h after treatment (HAT) than resistant biotypes. High differential (14)C-glyphosate translocation was observed at 12 HAT, so that >70% of the absorbed herbicide remained in the treated leaf in resistant biotypes, whereas 42% remained in the susceptible biotype at 96 HAT. Glyphosate was degraded to aminomethylphosphonic acid (AMPA), glyoxylate, and sarcosine by >90% in resistant biotypes, whereas a small amount of herbicide (up to 11%) was degraded by the susceptible biotype up to 168 HAT. Two amino acid changes were found at positions 182 and 310 in EPSPS, consisting of a proline to threonine and a tyrosine to cysteine substitution, respectively, in resistant biotypes. Therefore, absorption, translocation, metabolism, and gene mutation play an important role in the D. insularis glyphosate resistance. more...
- Published
- 2012
- Full Text
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10. Mechanism of resistance to ACCase-inhibiting herbicides in wild oat (Avena fatua ) from Latin America.
- Author
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Cruz-Hipolito H, Osuna MD, Dominguez-Valenzuela JA, Espinoza N, and De Prado R
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- Acetyl-CoA Carboxylase genetics, Mutation, Poaceae enzymology, Acetyl-CoA Carboxylase antagonists & inhibitors, Enzyme Inhibitors pharmacology, Herbicide Resistance genetics, Herbicides pharmacology, Poaceae genetics
- Abstract
Whole-plant response of two suspected resistant Avena fatua biotypes from Chile and Mexico to ACCase-inhibiting herbicides [aryloxyphenoxypropionate (APP), cyclohexanedione (CHD), and pinoxaden (PPZ)] and the mechanism behind their resistance were studied. Both dose-response and ACCase enzyme activity assays revealed cross-resistance to the three herbicide families in the biotype from Chile. On the other hand, the wild oat biotype from Mexico exhibited resistance to the APP herbicides and cross-resistance to the CHD herbicides, but no resistance to PPZ. Differences in susceptibility between the two biotypes were unrelated to absorption, translocation, and metabolism of the herbicides. PCR generated fragments of the ACCase CT domain spanning the potential mutations sited in the resistant and susceptible biotypes were sequenced and compared. A point mutation was detected in the aspartic acid triplet at the amino acid position 2078 in the Chilean biotype and in isoleucine at the amino acid position 2041 in the Mexican wild oat biotype, which resulted in a glycine triplet and an asparagine triplet, respectively. On the basis of in vitro assays, the target enzyme (ACCase) in these resistant biotypes contains a herbicide-insensitive form. This is the first reported evidence of resistance to pinoxaden in A. fatua. more...
- Published
- 2011
- Full Text
- View/download PDF
11. Differential susceptibility to glyphosate among the Conyza weed species in Spain.
- Author
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González-Torralva F, Cruz-Hipolito H, Bastida F, Mülleder N, Smeda RJ, and De Prado R
- Subjects
- Glycine pharmacology, Herbicide Resistance, Spain, Glyphosate, Conyza drug effects, Glycine analogs & derivatives, Herbicides pharmacology
- Abstract
Greenhouse and laboratory experiments were conducted to investigate differences in glyphosate susceptibility among three species of the genus Conyza introduced as weeds in Spain: tall fleabane (Conyza sumatrensis), hairy fleabane (Conyza bonariensis), and horseweed (Conyza canadensis). Plant material was obtained from seeds collected in weed populations growing in olive groves and citrus orchards in southern Spain, with no previous history of glyphosate application. Dose-response curves displayed ED(50) values of 2.9, 15.7, and 34.9 g ai ha(-1), respectively, for C. sumatrensis, C. bonariensis, and C. canadensis plants at the rosette stage (6-8 leaves). Significant differences were found among the three species in the glyphosate retention on leaves as well as the leaf contact angle. The species order according to glyphosate retention was C. sumatrensis > C. bonariensis > C. canadensis, while the mean contact angles of glyphosate droplets were 59.2, 65.5, and 72.9 degrees , respectively. There were no significant differences among species in the absorption of [(14)C]glyphosate (ranged from 37.4% for C. canadensis to 52.4% for C. sumatrensis), but the order among species was the same as glyphosate retention. The amount of radioactivity translocated from treated leaves was lower in C. canadensis as compared to the other two species (C. sumatrensis > C. bonariensis > C. canadensis). Combined, all of the studied parameters identified differential susceptibility to glyphosate among the Conyza species. Each species accumulated shikimate in leaf tissues following application of glyphosate at 200 g ai ha(-1). However, C. canadensis exhibited lower shikimate levels than the other two species at 168 h after herbicide application. For hairy fleabane, a greenhouse study explored its susceptibility to glyphosate at three developmental stages: rosette, bolting (stem height, 10-15 cm), and flowering. The ED(50) was lower at the rosette stage (15.7 g ai ha(-1)) as compared to bolting (86.6 g ai ha(-1)), with the highest ED(50) values occurring at flowering (117.5 g ai ha(-1)); plants at the earlier developmental stage retained more glyphosate. These results agree with field observations that plants at early developmental stages are more sensitive to glyphosate. more...
- Published
- 2010
- Full Text
- View/download PDF
12. Nontarget mechanims involved in glyphosate tolerance found in Canavalia ensiformis plants.
- Author
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Cruz-Hipolito H, Osuna MD, Heredia A, Ruiz-Santaella JP, and De Prado R
- Subjects
- Amaranthus physiology, Canavalia physiology, Glycine chemistry, Glycine pharmacology, Herbicides chemistry, Plant Leaves drug effects, Plant Leaves physiology, Shikimic Acid metabolism, Glyphosate, Amaranthus drug effects, Canavalia drug effects, Drug Resistance, Glycine analogs & derivatives, Herbicides pharmacology
- Abstract
A glyphosate-tolerant population of Canavalia ensiformis was collected in a cover crop in citrus orchards in Veracruz (Mexico), where glyphosate had been used for the first time. A susceptible Amaranthus hybridus L. population was collected from a nearby field that had never been treated with glyphosate. Dose-response experiments indicated a glyphosate tolerance ratio [ED(50)(C. ensiformis)/ED(50) (A. hybridus)] of 7.7. The hypothesis of a high level of glyphosate tolerance was provisionally corroborated on the basis of shikimate accumulation in both species. The susceptible population accumulated 6 times more shikimic acid in leaf tissue 96 h after glyphosate application than the tolerant leguminous crop. Two different physiological factors were involved in the glyphosate tolerance of this C. ensiformis population, which were confirmed by [(14)C]glyphosate, being a lack of penetration of glyphosate through the cuticle of the leguminous plants and an impaired herbicide translocation to the roots and the rest of shoots. This paper reports that two different nontarget site-based mechanisms, limited absorption and reduced translocation, contribute to the glyphosate tolerance found in C. ensiformis. more...
- Published
- 2009
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13. Resistance mechanism to bensulfuron-methyl in biotypes of Scirpus mucronatus L. collected in Chilean rice fields.
- Author
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Cruz-Hipolito H, Osuna MD, Vidal RA, and De Prado R
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- Acetolactate Synthase antagonists & inhibitors, Acetolactate Synthase chemistry, Amino Acid Sequence, Chile, Cyperaceae enzymology, Cyperaceae genetics, Enzyme Inhibitors pharmacology, Seeds drug effects, Cyperaceae drug effects, Herbicide Resistance, Oryza growth & development, Sulfonylurea Compounds pharmacology
- Abstract
Two biotypes of Scirpus mucronatus not controlled with the herbicide bensulfuron-methyl in rice fields were characterized by using field, greenhouse, and laboratory techniques. Seeds were collected in two rice areas [Parral (R1) and Linares (R2)], where bensulfuron-methyl at 150 g ha(-1) did not control S. mucronatus. A third seed sample of S. mucronatus susceptible (S) to bensulfuron-methyl was collected in an area from Chile. The dose-response studies confirmed resistance to bensulfuron-methyl in R1 and R2 S. mucronatus biotypes; ratios (R/S) of the ED(50) values of resistant to susceptible plants were 1719 and 1627 for R1 and R2, respectively. The biotype R1 also showed strong cross-resistance (ratios ranging from 1719 to 43) to sulfonylureas (bensulfuron-methyl, cyclosulfamuron, ethoxysulfuron, imazosulfuron, and pyrazosulfuron-ethyl) and imidazolinone (imazamox) and a weak cross-resistance (ratio of 1.705) to pyrimidinyloxybenzoates (bispyribac-sodium), all ALS inhibiting herbicides used in rice. Absorption, translocation, and metabolism results did not explain the differences in susceptibility among biotypes. The in vitro assays confirmed cross-resistance to all ALS inhibitors tested and the level of cross resistance was bensulfuron-methyl > imazosulfuron ≫ cyclosulfamuron ≫ pyrazosulfuron-ethyl ≫ ethoxysulfuron > imazamox ≫ bispiribac-sodium. Molecular studies demonstrated that the Pro197His amino acid substitution on the ALS enzyme could explain the loss of affinity for the ALS-inhibiting herbicides. more...
- Published
- 2009
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14. Effect of soil type on wines produced from Vitis vinifera L. cv. Grenache in commercial vineyards.
- Author
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de Andrés-de Prado R, Yuste-Rojas M, Sort X, Andrés-Lacueva C, Torres M, and Lamuela-Raventós RM
- Subjects
- Adult, Humans, Middle Aged, Phenols analysis, Stilbenes analysis, Taste, Time Factors, Water, Fruit chemistry, Soil analysis, Vitis chemistry, Vitis growth & development, Wine analysis
- Abstract
In recent years, the wine industry has become increasingly interested in the influence of the terroir characteristics on the features of a wine and, in particular, the mechanisms by which a soil influences wine quality. Among published papers on this topic, most merely describe the effect of the soil; few explain it. In this study were conducted a sensory evaluation and phenolic composition and stilbene concentration tests in order to analyze the effects of soil on wine. Significant differences were found in the results of the tests conducted on two vineyards during two consecutive harvests in 2004 and 2005. The results, in line with previous reports, show that the more fertile of the two vineyards, which was also the one with the greatest water-holding capacity, produced wines that presented significantly lower color intensity and shade, as well as lower total phenolic composition and a smaller quantity of hydroxycinnamic compounds. In 2004, these wines presented significantly higher trans-resveratrol content, due to a fungal attack that was favored by the vineyard's soil characteristics. Extreme drought conditions in 2005 had a marked impact on the characteristics of the wines, increasing wavelength measurements significantly and reducing stilbene concentrations. Finally, sensory evaluations revealed significant differences between the wines produced on the two vineyards in both years for five of the seven attributes evaluated. No significant differences were found from one year to the next between the wines produced from the same vineyard, indicating that the attributes of these wines were maintained despite markedly different vintage conditions. more...
- Published
- 2007
- Full Text
- View/download PDF
15. Lolium rigidum, a pool of resistance mechanisms to ACCase inhibitor herbicides.
- Author
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De Prado JL, Osuna MD, Heredia A, and De Prado R
- Subjects
- Halogenated Diphenyl Ethers, Lolium ultrastructure, Microscopy, Electron, Scanning, Phenyl Ethers pharmacology, Spain, Acetyl-CoA Carboxylase antagonists & inhibitors, Drug Resistance, Enzyme Inhibitors pharmacology, Herbicides pharmacology, Lolium drug effects
- Abstract
Three diclofop-methyl (DM) resistant biotypes of Lolium rigidum (R1, R2, and R3) were found in different winter wheat fields in Spain, continuously treated with DM, DM + chlortoluron, or DM + isoproturon. Herbicide rates that inhibited shoot growth by 50% (ED50) were determined for DM. There were found that the different biotypes exhibited different ranges of resistance to this herbicide; the resistant factors were 7.2, 13, and 36.6, respectively. DM absorption, metabolism, and effects on ACCase isoforms were examined in these biotypes of L. rigidum. The most highly resistant, biotype R3, contained an altered isoform of ACCase. In biotype R2, which exhibited a medium level of resistance, there was an increased rate of oxidation of the aryl ring of diclofop, a reaction most likely catalyzed by a cytochrome P450 enzyme. In the other biotype, R1, DM penetration was significantly less than that observed in the resistant (R2 and R3) and susceptible (S) biotypes. Analysis of the leaf cuticle surface by scanning electron microscopy showed a greater epicuticular wax density in the leaf cuticles of biotype R1 than in the other biotypes. more...
- Published
- 2005
- Full Text
- View/download PDF
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