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Start Over Author "Ni, Hui" Journal journal of agricultural and food chemistry
8 results on '"Ni, Hui"'

4. Heterologous Expression and Characterization of a New Clade of Aspergillusα-L-Rhamnosidase Suitable for Citrus Juice Processing

Author

Li, Lijun, Gong, Jianye, Wang, Song, Li, Guiling, Gao, Ting, Jiang, Zedong, Cheng, Yi-Sheng, Ni, Hui, and Li, Qingbiao

Abstract

α-L-Rhamnosidase is a glycoside hydrolase capable of removing naringin from citrus juice. However, α-L-rhamnosidases always have broad substrate spectra, causing negative effects on citrus juice. In this study, a α-L-rhamnosidase-expressing fungal strain, JMU-TS529, was identified, and its α-L-rhamnosidase was characterized. As a result, JMU-TS529 was identified as Aspergillus tubingensisvia morphological and molecular characteristics. The predicted protein sequence shared an amino acid identity of less than 30% with previously characterized α-L-rhamnosidases. The optimal pH and temperature were 4.0 and 50–60 °C, respectively. Most importantly, the α-L-rhamnosidase showed a strong ability to hydrolyze naringin but scarcely acted on other substrates. Furthermore, the enzyme could efficiently remove naringin from pomelo juice without changing its attractive aroma. These results indicate that the present enzyme represents a new clade of Aspergillusα-L-rhamnosidase that is desirable for debittering citrus juice, providing a better alternative for improving the quality of citrus juice.

Published
2019
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