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Start Over Author "Wu, Dandan" Journal journal of agricultural and food chemistry
8 results on '"Wu, Dandan"'

7. Multiomics Reveals Mechanisms of Alternaria oxytropisInhibiting Pathogenic Fungi in Oxytropis ochrocephala

Author

Fu, Keyi, Schardl, Christopher L., Cook, Daniel, Cao, Xuanli, ling, Ning, He, Chunyu, Wu, Dandan, Xue, Longhai, Li, Yanzhong, and Shi, Zunji

Abstract

Endophytic fungi can benefit the host plant and increase the plant resistance. Now, there is no in-depth study of how Alternaria oxytropis(A. oxytropis) is enhancing the ability of inhibiting pathogenic fungi in Oxytropis ochrocephala(O. ochrocephala). In this study, the fungal community and metabolites associated with endophyte-infected (EI) and endophyte-free (EF) O. ochrocephalawere compared by multiomics. The fungal community indicated that there was more A. oxytropis, less phylum Ascomycota, and less genera Leptosphaeria, Colletotrichum, and Comoclathrisin the EI group. As metabolic biomarkers, the levels of swainsonine and apigenin-7-O-glucoside-4-O-rutinoside were significantly increased in the EI group. Through in vitro validation experiments, swainsonine and apigenin-7-O-glucoside-4-O-rutinoside can dramatically suppress the growth of pathogenic fungi Leptosphaeria sclerotioidesand Colletotrichum americae-borealisby increasing the level of oxidative stress. This work suggested that O. ochrocephalacontaining A. oxytropiscould increase the resistance to fungal diseases by markedly enhancing the content of metabolites inhibiting pathogenic fungi.

Published
2024
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8. Role of RNA Modifications, Especially m6A, in Aflatoxin Biosynthesis of Aspergillus flavus .

Author

Yang C, Wu D, Lin H, Ma D, Fu W, Yao Y, Pan X, Wang S, and Zhuang Z

Subjects
Adenine metabolism, RNA metabolism, Aspergillus flavus genetics, Aspergillus flavus metabolism, Aflatoxins metabolism
Abstract

RNA modifications play key roles in eukaryotes, but the functions in Aspergillus flavus are still unknown. Temperature has been reported previously to be a critical environmental factor that regulates the aflatoxin production of A. flavus , but much remains to be learned about the molecular networks. Here, we demonstrated that 12 kinds of RNA modifications in A. flavus were significantly changed under 29 °C compared to 37 °C incubation; among them, m6A was further verified by a colorimetric method. Then, the transcriptome-wide m6A methylome and m6A-altered genes were comprehensively illuminated through methylated RNA immunoprecipitation sequencing and RNA sequencing, from which 22 differentially methylated and expressed transcripts under 29 °C were screened out. It is especially notable that AFCA_009549, an aflatoxin biosynthetic pathway gene ( aflQ ), and the m6A methylation of its 332nd adenine in the mRNA significantly affect aflatoxin biosynthesis in A. flavus both on media and crop kernels. The content of sterigmatocystin in both Δ aflQ and aflQ A332C strains was significantly higher than that in the WT strain. Together, these findings reveal that RNA modifications are associated with secondary metabolite biosynthesis of A. flavus .

Published
2024
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