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Penelitian ini bertujuan untuk mengkaji keberhasilan sambung samping tanaman durian pada berbagai taraf konsentrasi IAA dan umur batang bawah yang berbeda. Penelitian disusun dengan rancangan acak lengkap faktorial dengan faktor pertama adalah konsentrasi auksin IAA ( 0, 75 da 150 ppm) dan faktor kedua adalah umur batang bawah ( 6, 9, dan 12 bulan). Masing-masing perlakuan diulang 5 kali. Parameter yang diamati yaitu waktu keberhasilan sambungan, waktu pecah tunas, panjang tunas, jumlah daun terbuka, dan lebar tajuk. Data dianalisis ragam dan dilanjutkan uji DMRT ( Duncan Multiple Range Test ). Hasil penelitian menunjukkan bahwa tidak ada pengaruh pada taraf konsentrasi IAA sedangkan taraf umur batang bawah memberikan pengaruh nyata (P

Penelitian ini bertujuan untuk mengkaji pengaruh penambahan BAP dan IAA pada media pengakaran kultur jaringan terhadap pertumbuhan tanaman pisang raja bulu (Musa paradisiaca). Penelitian dilaksanakan di Laboratorium Kultur Jaringan Kebun Benih Hortikultura Salaman, Magelang pada bulan Juni hingga Agustus 2018. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) faktorial dengan 4 (empat) kali ulangan. Faktor pertama adalah hormon BAP dengan konsentrasi masing-masing 0 ; 0,5 ; 1 ; 1,5 ; dan 2 ppm, faktor kedua yaitu hormon IAA dengan konsentrasi 0, 1, 2, 3, dan 4 ppm. Kombinasi perlakuan sebanyak 25 dengan 4 kali ulangan, sehingga terdapat 100 unit percobaan yang setiap unit percobaan terdiri dari 5 planlet pisang raja bulu. Hasil penelitian menunjukkan bahwa terdapat pengaruh interaksi perlakuan BAP dan IAA terhadap parameter pertumbuhan planlet pisang raja bulu. Kombinasi BAP 1 ppm + IAA 3 ppm memberikan pengaruh terbaik pada jumlah daun dan diameter batang, sedangkan kombinasi BAP 0,5 ppm + IAA 4 ppm memberikan pengaruh terbaik pada jumlah akar dan panjang akar. Kombinasi BAP 1,5 ppm + IAA 4 ppm memberikan pengaruh terbaik pada tinggi planlet. Kesimpulan dari penelitian ini adalah zat pengatur tumbuh BAP 0,5 – 1,5 ppm + IAA 3 – 4 ppm direkomendasikan untuk media pengakaran kultur jaringan pisang raja bulu. Kata kunci: in vitro, BAP, IAA, plantlet, pisang

Penelitian ini bertujuan untuk mengetahui respon pertumbuhan beberapa varietas benih bawang merah terhadap beberapa dosis GA 3 terhadap pertumbuhan bawang merah. Penelitian ini dilaksanakan pada tanggal 5 Maret – 6 Juli 2018 di Screen House Agroecotechnopark dan Laboratorium Fisiologi dan Pemuliaan Tanaman, Universitas Diponegoro, Semarang. Penelitian ini akan menggunakan Rancangan Acak Lengkap Faktorial 4x3 dengan 4 kali ulangan. Faktor pertama yaitu dosis GA 3 terhadap terdiri atas 4 taraf yaitu M0: 0 ppm, M1: 50 pm, M2:100 ppm dan M3: 200 ppm. Faktor kedua yaitu beberapa varietas benih bawang merah terdiri atas 3 taraf yaitu V1: Bima, V2: Tutuk dan V3: Lokananta . Parameter yang diamati yaitu Indek vigor, daya kecambah, potensi tumbuh maksimum, tinggi tanaman, jumlah daun, jumlah anakan dan berat basah total tanaman. Data dianalisis ragam dan di uji lanjut dengan Duncan’s Multiple Range Test (DMRT) . Hasil penelitian menunjukkan perlakuan dosis GA 3 100 ppm dengan varietas Lokananta memberikan hasil nyata lebih tinggi dibanding perlakuan lainnya, pada parameter indeks vigor dan daya kecambah. Perlakuan dosis GA 3 50 ppm dengan varietas tuktuk memberikan hasil nyata lebih tinggi dibanding perlakuan lainnya, pada jumlah daun dan anakan bawang merah. Kata Kunci : Allium cepa L ., true shallot seed , giberelin, pertumbuhan.

Penelitian bertujuan untuk mengkaji jenis naungan yang sesuai dan sasaran pemberian paklobutrazol yang tepat untuk pertumbuhan serta pembungaan anggrek Dendrobium sp. Penelitian menggunakan Pooled Design dengan perlakuan naungan : N 1 (naungan 30%) , N 2 (naungan 50%) , N 3 (naungan 70%), serta cara pengaplikasian paklobutrazol : P0 (kontrol), P1 (disemprotkan pada akar), P2 (disemprotkan pada daun), P3 (disemprotkan pada akar dan daun) , diulang sebanyak 3 kali. Parameter yang diamati meliputi tinggi tanaman , diameter batang, klorofil total , dan waktu inisiasi bunga anggrek . Data dianalisis ragam dan uji lanjut dengan BNT pada taraf α = 5%. Hasil penelitian menunjukkan bahwa naungan 50% berpengaruh pada tinggi tanaman (26,97 cm) dan diameter batang (1,57 cm), naungan 70% juga berpengaruh terhadap tinggi tanaman anggrek (29,30 cm) dan klorofil total (0,55 mg/g). Paklobutrazol tidak memiliki pengaruh terhadap tinggi tanaman dan diameter batang. Paklobutrazol hanya berpengaruh terhadap kandungan klorofil total. Interaksi antara perlakuan naungan 70% dan pemberian paklobutrazol pada daun, menunjukkan kadar klorofil tertingi sebesar 0,71 mg/g. Faktor pemberian naungan dan paklobutrazol tidak mempengaruhi pembungaan anggrek, tapi inisiasi bunga mulai terlihat di beberapa tanaman. Waktu inisiasi bunga terjadi saat hari ke 71 – 126 setelah perlakuan. Semua inisiasi bunga tidak dapat berkembang menjadi rangkaian bunga. Kata kunci: anggrek, paklobutrazol, naungan, pertumbuhan, pembungaan. ABSTRACT The research aimed to examine the appropriate shade types and target of paclobutrazol giving for growth and flowering of Dendrobium sp. The research used Pooled Design with shade treatment: N1 (30% shade), N2 (50% shade), N3 (70% shade), treatment of paclobutrazol application: P0 (control), P1 (sprayed on roots), P2 (sprayed on leaf), P3 (sprayed on roots and leaf), replicated three times. Parameters measured were plant height, stem diameter, total chlorophyll, and the initiation time of orchid flowers. Data analyzed with anova and followed by LSD at α = 5%. The results showed that 50% shade affect plant height (26.97 cm) and stem diameter (1.57 cm), the 70% shade also affect plant height (29.30 cm) and total chlorophyll (0.55 mg/g). Paclobutrazol has no effect on plant height and stem diameter. Paclobutrazol only affect the total chlorophyll content. The interaction between the shade treatment of 70% and the application of paclobutrazol in leaves, showed the highest total chlorophyll content of 0.71 mg/g. Shading and paclobutrazol factors do not affect the flowering of orchids, but the flower initiation begins to be seen in some plants. The initiation time of flower occurs at days 71 to 126 after treatment. All flowers initiation didn’t develop into a flower arrangement. Keyword: orchid, shade, paclobutrazol, growth, flowering

The objective of this research was to study the growth and production of peanut at phosphate fertilization and vesicular-arbuscular mycorrhizal fungi (MVA) inoculation. This research was conducted in green house and Laboratory of P lant P hysiology and B reeding UNDIP, and Laboratory of Micology UGM from April 16 th to August 15 th 2017. The research was assigned in completely randomized factorial design with the first factor was the phosphate fertilization control , Triple Super Phosphate (TSP) and rock phosphate (BP) and second factor was without vesicular-arbuscular mycorrhizal fungi (MVA) inoculation and with vesicular-arbuscular mycorrhizal fungi (MVA) inoculation. The results showed that TSP fertilizationhad significant effect onplant height , number of pods, weight of pods, weight of seed and total P content of soil. Fertilization of BP had significant effect onnumber of pods, weight of pods, weight of seed, total P content of soil and total P of peanut hay. Inoculation of MVA had significant effect onplant height , number of pods, weight of pods, weight of seed, total P content of soil, percentage of colonization on plant roots and number of MVA spores. Keyword: peanut , vesicular-arbuscular mycorrhizal fungi , phosphorus fertilization ABSTRAK Penelitian bertujuan untuk mengkaji pertumbuhan dan produksi tanaman kacang tanah ( Arachis hypogaea L. ) pada berbagai jenis pemupukan fosfat dan inokulasi cendawan mikoriza vesikular-arbuskular (MVA). Penelitian dilakukan pada tanggal 16 April – 15 Agustus 2017 di green house dan Laboratorium Fisiologi dan Pemuliaan Tanaman UNDIP, serta Laboratorium Mikologi UGM. Penelitian disusun dengan rancangan acak lengkap faktorial dengan faktor pertama adalah kontrol, pemupukan Triple Super Phosphate (TSP), Batuan Fosfat (BP) dan faktor kedua adalah tanpa inokulasi cendawan MVA dan dengan inokulasi cendawan MVA . Hasil penelitian menunjukkan bahwa perlakuan pemupukan TSP berpengaruh nyata terhadap tinggi tanaman, jumlah polong, bobot polong, bobot biji dan kadar P total tanah. Pemupukan BP berpengaruh nyata terhadap jumlah polong, bobot polong, bobot biji, kadar P total tanah dan kandungan P jerami tanaman kacang tanah. Inokulasi cendawan MVA berpengaruh nyata terhadap tinggi tanaman, jumlah polong, bobot polong, bobot biji, kadar P total tanah, persentase kolonisasi pada akar tanaman dan jumlah spora cendawan MVA. Kata kunci: kacang tanah , cendawan mikoriza vesikular-aruskular , pemupukan fosfat

Mutation is one of plant breeding ways to expand genetic diversity. The purpose of the research was to evaluate the effect of sodium azide mutagen on soybean variety Dering 1 at saline and non-saline soil . The research was arranged in Factorial Design based on Completely Randomized Design with 2 factors. The first factor was doses of Sodium Azide consisted of 0 mM, 0.05 mM, 0.1 mM, 0.2 mM, dan 0.4 mM, 0.8 mM, 1.6 mM, 3.2 mM, 6.4 mM, 12.8 mM, and 25.6 mM. The second factor was salinity levels consisted of 0 dS/m, 2 dS/m and 5 dS/m. Parameters measured were plant height, number of leaves, number of pods, weight of pod, number of seeds, and weight of seeds per plant on M1 generation. Result showed that lethal dosage (LD) 50 was obtained at 0,663 mM. Sodium azide mutagent caused diversity of plant height, number of leaves at saline and non-saline soil. There was 10 plants and 3 plants that was classified as tolerant and most tolerant at saline soil (2 dS/m) respectively. Keywords : soybean, sodium azide, saline soil ABSTRAK Mutasi adalah salah satu cara pemuliaan tanaman untuk memperluas kergaman genetik. Tujuan penelitian untuk mengevaluasi pengaruh mutagen sodium azida terhadap kedelai varietas Dering 1 pada tanah salin dan non-salin. Rancangan percobaan yang digunakan di greenhouse adalah Percobaan Faktorial dengan dasar Rancangan Acak Lengkap yang terdiri dari 2 faktor. Faktor pertama adalah dosis mutagen kimia Sodium Azide (SA) yang terdiri dari 11 taraf perlakuan yaitu 0 mM, 0,05 mM, 0,1 mM, 0,2 mM, dan 0,4 mM, 0,8 mM, 1,6 mM, 3,2 mM, 6,4 mM, 12,8 mM, 25,6 mM. Faktor kedua adalah tingkat salinitas yaitu 0 dS/m, 2 dS/m dan 5 dS/m. Parameter yang diamati adalah tinggi tanaman, jumlah daun, jumlah polong, berat polong, jumlah biji, dan berat biji per tanaman pada generasi M1. Hasil penlitian menunjukkan bahwa dosis letal median (LD50) diperoleh pada 0,663 mM. Mutagen sodium azida menyebabkan keragaman tinggi tanaman, jumlah daun pada tanah salin dan non-salin. Terdapat 10 tanaman dan 3 tanaman yang masing-masing tergolong tahan dan sangat tahan pada tanah salin (2 dS/m). Kata kunci : kedelai, sodium azida, tanah salin

Cherry tomato have various colour, shape and nutritious fruit. The aim of this research was to make population for genetic selection and to know about xenia and metaxenia effect on tomato crossing. The crossing was assigned in dialel crossing combination with Ranti tomato, Cherry tomato var. Grape, Red Pear and Indigo Rose as a crossing parents. The crossing combination was Ranti x Grape, Ranti x Red Pear, Ranti x Indigo Rose, Grape x Ranti, Grape x Red Pear, Grape x Indigo Rose, Indigo Rose x Grape, Indigo Rose x Ranti, Indigo Rose x Red Pear. Parameter measured were crossing ability, fruit set ability, fruit ripening age, seed number and fruit shape. The result showed that Grape x Red Pear crossing combination had the highest crossing ability and Indigo Rose x Red Pear had the highest fruit set ability. Xenia and metaxenia effects were showed in fruit ripening age, seed number and fruit shape. Genotype of Red Pear x Indigo Rose was found as superior genotype candidate for ripening age, seed number and had highest percentage of fruit shape alteration. Keywords : Hybridization, xenia, metaxenia, tomato, cherry tomato.

Jatropha curcas is a plant that is used as an alternative energy for fossil fuel replacement because of it’s high oil content. Jatropha curcas seeds have 30-40% oil content. The purpose of the study was to improve the seed quality and to enhance seedling growth of Jatropha c urcas after storage using plant growth regulator. This study used factorial design based on Completely Randomized Design with two factors and 3 replications . The first factor was seed storage which consist of 2 levels : A1 = seed storage in 2015, A2 = seed storage in 2016. The second factor was plant growth regulator level which consists of 6 levels : B1=GA+NAA 0 ppm, B2= GA+NAA 20 ppm, B3= GA+ NAA 40 ppm, B4= GA+NAA 60 ppm, B5 = GA+NAA 80 PPM, B6 = GA+NAA 100 ppm. Parameters measured were seed germination, vigor index, growth rate, plant height and the number of leaves. The data were analyzed statistically by analysis of variance and followed by Tukey test and Polynomial Orthogonal. The result showed that seed germination, vigor index and growth rate were significantly affected by storage treatment and invigoration using plant growth regulator treatment. Invigoration using auxin and gibberelli c acid 40 ppm has the highest result of seed germinat i on, vigor index and growth rate. There was no influence of seed storage and invigoration with plant growth regulator treatment on plant height and number of leaves. Keywords : Jatropha curcas, invigoration, plant growth regulator.

The purpose of this research was to study the influence of various concentrations of IAA and BAP on early growth budding of guava (Psidium guajava L.) Var. Kristal in side grafting propagation method. The experiment used completely randomized factorial design. The first factor was various concentrations of IAA (0, 100, and 200 ppm) and the second factor was various concentrations of BAP (0, 100, and 200 ppm). Each treatment was repeated three times. Parameters observed were the time of bud break, time of shoot emergence, and shoot length. The data were analyzed by variance and continued analyzed by Duncan Multiple Range Test. The results showed that the level of IAA concentration gave effect (P

The purpose of the research was to study the concentration of NaOCl and drying method during the extraction process for germination and growth of tomato seedlings. This study used Completely Randomized Design with two factors with three replicates . The first factor was soaking the seeds with NaOCl concentration s: NaOCl 0% (control), NaOCl 6%, NaOCl 9% and NaOCl 13%. The second factor was the drying method s: without drying (control), natural drying and drying using oven. The parameters observed were germination, seed growth rate, maximum growth potential, leaf size, leaf area, plant height, plant growth rate and relative plant growth rate. The data were analyzed using vari ance analysis and continued by Duncan Multiple Range Test (DMRT) of 5%. The result of variance analysis showed that immersion treatment using NaOCl and drying method had not given interaction during seed germination and seedling growth. Soaking with 9% NaOCl showed the best result of germination process and seedling growth whereas the best drying method was naturally because it d id not cause impermeability of seeds. Keyword : Tomato, seed, drying, NaOCl, seed viability

Beauveria bassiana is one of the pathogens that can be used as an environmentally friendly bio pesticide, as it does not produce harmful residues and does not cause pest resistance. This research was conducted in the village of Campursari village, District of Bulu, Temanggung Regency. The purpose of this research was assess the effectiveness of Beauveria bassiana application in order to suppress the population of brown planthopper and adult rice bug in rice plants and to find out rice plants production. The research used factorial randomized design which consists of two treatments. The first treatment was the application of Beauveria bassiana with controlled doses by 0, 100, 150 and 200 g/14 l. The second treatment was the application interval between 7, 10, and 14 days. The parameters observed were brown planthopper populations, adult rice bug population, plant height, number of tillers and grain production. The results showed that application with doses of 200 g/14 l and 7 day interval application was effective to suppress population of brown planthopper and adult rice bug and also saved grain production by 1,20 kg to 7,02 kg/m 2 depending on application dosage. Keywords : planthopper, adult rice bug, Beauveria , doses, application interval

Penelitian bertujuan mengetahui dosis ekstrak daun kelor yang dapat meningkatkan jumlah anakan tebu. Rancangan penelitian yang digunakan adalah rancangan acak lengkap faktorial dua faktor dengan 5 kali ulangan. Faktor pertama yaitu perlakuan dosis ekstrak daun kelor (0, 10, 20, dan 30) dengan 10 ml/l larutan sitokinin sintetik, dan faktor kedua adalah 3 varietas tanaman tebu (Varietas 1 = BM 9514, varietas 2 = BM 9044, dan varietas 3 = BM 9605). Parameter pengamatan yaitu daya kecambah, tinggi tanaman, jumlah anakan, berat segar tanaman, berat kering tanaman, berat segar tajuk, berat kering tajuk, berat segar akar, berat kering akar, dan volume akar. Data dianalisis ragam dan di uji lanjut dengan Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukan bahwa daya kecambah tertinggi pada varietas BM 9605 yaitu 97,4%. Perlakuan ekstrak daun kelor memiliki pengaruh terhadap volume akar dan terdapat interaksi dosis dan varietas. perlakuan varietas memiliki pengaruh terhadap tinggi tanaman dan jumlah anakan tanaman tebu. Responsibilitas tanaman tebu terhadap perlakuan ekstrak daun kelor memiliki hasil yang berbeda. varietas BM 9044 sudah merespon perlakuan dosis ekstrak daun kelor dengan dosis 10 ml/l sedangkan Varietas BM 9605 merespon perlakuan dosis ekstrak daun kelor dengan dosis 20 ml/l, dan Varietas BM 9514 merespon perlakuan dosis ekstrak daun kelor dengan dosis 30 ml/l.

Moss Rose (Portulaca grandiflora Hook) is one of the ornamental plants and medicinal plants. The purpose of this research was to evaluate cytological and morphological responses of Moss Rose (Portulaca grandiflora) with colcichine treatment. Colcichine treatment was done with drop method technique in different concentrations and different frequency of application. This research used Completely Randomized Design in 4x3 factorial arrangement and used descriptive analysis to compare diploid plant and tetraploid plant characters. The first factor was variation of cholcichine concentration which were K0 (0%), K1 (0.1%), K2 (0.3%), and K3 (0.6%). The second factor was variation of application frequency which were A1 (2 drop per day for a day), A2 (2 drop per day for 2 days), and A3 (2 drop per day for 3 days). The observed parameters were shoot response by colcichine treatment, number of chromosomes, length and width of stomata, stomata density, stem diameter, flower diameter, plant high, fresh weight, number of branches, and number of leaves. The result showed that Moss Rose’s shoot was intolerant with colcichine treatment in high concentration and long time application. The tetraploid plants was randomly produced by a number of treatment which were 0.1% colcichine 2 drops for 3 days, 0.3% colcichine 2 drops for 2 days, 0.6% colcichine 2 drops for 2 days, and 0.6% colcichine 2 drops for 3 days. The shoot’s growth was inhibited. The induced of tetraploid plant in Moss Rose was accompanied by double chromosome number as 2n=4x=36, larger stomata (length and width), lower stomata density, and higher morphological characters. Moss Rose (Portulaca grandiflora Hook) is one of the ornamental plants and medicinal plants. The purpose of this research was to evaluate cytological and morphological responses of Moss Rose (Portulaca grandiflora) with colcichine treatment. Colcichine treatment was done with drop method technique in different concentrations and different frequency of application. This research used Completely Randomized Design in 4x3 factorial arrangement and used descriptive analysis to compare diploid plant and tetraploid plant characters. The first factor was variation of cholcichine concentration which were K0 (0%), K1 (0.1%), K2 (0.3%), and K3 (0.6%). The second factor was variation of application frequency which were A1 (2 drop per day for a day), A2 (2 drop per day for 2 days), and A3 (2 drop per day for 3 days). The observed parameters were shoot response by colcichine treatment, number of chromosomes, length and width of stomata, stomata density, stem diameter, flower diameter, plant high, fresh weight, number of branches, and number of leaves. The result showed that Moss Rose’s shoot was intolerant with colcichine treatment in high concentration and long time application. The tetraploid plants was randomly produced by a number of treatment which were 0.1% colcichine 2 drops for 3 days, 0.3% colcichine 2 drops for 2 days, 0.6% colcichine 2 drops for 2 days, and 0.6% colcichine 2 drops for 3 days. The shoot’s growth was inhibited. The induced of tetraploid plant in Moss Rose was accompanied by double chromosome number as 2n=4x=36, larger stomata (length and width), lower stomata density, and higher morphological characters.