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In this paper, the resistance and the gene homology of Acinetobacter calcoaceticus-baumannii strains which were isolated clinically from the Fourth and the Third People's Hospital of Wuxi, China was studied. The prevalence and distribution was observed and recorded. The susceptibility testing was performed with agar dilution technique and compared with the genotype was investigated with randomly amplified polymorphic DNA (RAPD) technique. To these strains, the resistant rates of Ampicillin, Piperacillin, Cefazolin, Nitrofurantoin were 100%, those of Imipenem, Meropenem and Cefoperazone/Sulbactam were 27%, 15% and 12%, respectively. The resistant rates of other β-lactams and quinolones were higher than 60%. The resistant rates of aminoglycosides were higher than 75%. The type A and D included 35 strains and 22 strains, respectively. The same genotype separated from the same and the different endemic areas. It is sensitive to Imipenem, Meropenem and Cefoperazone/Sulbactam among the 100 strains of Acinetobacter calcoaceticus –baumannii. Maybe the 2 strains are Acinetobacter calcoaceticus and 98 strains are Acinetobacter baumannii. The type A and D are the major types, which show cross-transmission in two hospitals in Wuxi.

In this paper, the resistance and the gene homology of Acinetobacter calcoaceticus-baumannii strains which were isolated clinically from the Fourth and the Third People's Hospital of Wuxi, China was studied. The prevalence and distribution was observed and recorded. The susceptibility testing was performed with agar dilution technique and compared with the genotype was investigated with randomly amplified polymorphic DNA (RAPD) technique. To these strains, the resistant rates of Ampicillin, Piperacillin, Cefazolin, Nitrofurantoin were 100%, those of Imipenem, Meropenem and Cefoperazone/Sulbactam were 27%, 15% and 12%, respectively. The resistant rates of other β-lactams and quinolones were higher than 60%. The resistant rates of aminoglycosides were higher than 75%. The type A and D included 35 strains and 22 strains, respectively. The same genotype separated from the same and the different endemic areas. It is sensitive to Imipenem, Meropenem and Cefoperazone/Sulbactam among the 100 strains of Acinetobacter calcoaceticus –baumannii. Maybe the 2 strains are Acinetobacter calcoaceticus and 98 strains are Acinetobacter baumannii . The type A and D are the major types, which show cross-transmission in two hospitals in Wuxi.

A novel optimal multilevel thresholding algorithm for image segmentation is presented to deal with RAPD Fingerprint of acinetobacter calcoaceticus-baumannii. The proposed algorithm is an artificial immune network used to optimize multilevel thresholds of image segmentation. In addition, a fast fuzzy clustering technique is used to improve efficiency of the algorithm. Experimental results show that the algorithm can effectively extract fingerprint from the images to facilitate genotyping analysis of RAPD fingerprinting of acinetobacter calcoaceticus-baumannii.