1. Evolution of pathologic T-cell subsets in patients with atopic dermatitis from infancy to adulthood
- Author
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Amy S. Paller, Tali Czarnowicki, Talia Canter, Stephanie M. Rangel, Taylor Erickson, Joseph Han, Hyun Je Kim, Emma Guttman-Yassky, Rachel Lefferdink, James G. Krueger, Naoya Kameyama, Ana B. Pavel, Helen He, and Yeriel Estrada
- Subjects
Adult ,Male ,0301 basic medicine ,Adolescent ,medicine.medical_treatment ,T cell ,Immunology ,Eczema Area and Severity Index ,Article ,Dermatitis, Atopic ,03 medical and health sciences ,Th2 Cells ,0302 clinical medicine ,Humans ,Immunology and Allergy ,Medicine ,SCORAD ,Child ,medicine.diagnostic_test ,business.industry ,Innate lymphoid cell ,Infant, Newborn ,Infant ,HLA-DR Antigens ,Atopic dermatitis ,Th1 Cells ,medicine.disease ,030104 developmental biology ,Cytokine ,medicine.anatomical_structure ,Child, Preschool ,Cytokines ,Biomarker (medicine) ,Female ,business ,CD8 ,030215 immunology - Abstract
BACKGROUND: The circulating immune phenotype was defined in adults and young children with early atopic dermatitis/AD, but chronologic changes in blood of AD infants and children through adolescence have not been explored. OBJECTIVE: To compare immune activation and cytokine polarization in blood of 0–5y/o (n=39), 6–11y/o (n=26), 12–17y/o (n=21) and ≥18y/o (n=43) patients with AD vs. age-matched controls. METHODS: Flow cytometry was used to measure interferon-γ, interleukin (IL)-9, IL-13, IL-17, and IL-22 cytokines in CD4(+)/CD8(+) T-cells, with ICOS and HLA-DR defining mid- and long-term T-cell activation, respectively, within skin-homing/CLA(+) vs. systemic/CLA(−) T-cells. Unsupervised clustering differentiated patients based on their blood biomarker frequencies. RESULTS: While CLA(+) Th1 frequencies were significantly lower in infants with AD vs. all older patients (P
- Published
- 2020
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