1. Dexmedetomidine protects against lung ischemia-reperfusion injury by the PI3K/Akt/HIF-1[alpha] signaling pathway
- Author
-
Zhang, Wei, Zhang, Jia-Qiang, Meng, Fan-Min, and Xue, Fu-Shan
- Subjects
Dexmedetomidine -- Dosage and administration -- Complications and side effects ,Protein kinases -- Physiological aspects -- Genetic aspects -- Research ,Reperfusion injury -- Physiological aspects -- Genetic aspects -- Drug therapy -- Research ,Cellular signal transduction -- Physiological aspects -- Genetic aspects -- Research ,Health - Abstract
Purpose To evaluate the role of the phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt)/hypoxia-inducible factor 1[alpha] (HIF-1[alpha]) signaling pathway in the protection by dexmedetomidine against lung ischemia-reperfusion injury (IRI) in rats. Methods Forty-eight male Sprague-Dawley rats weighing 250-350 g were randomly divided into six groups (n = 8 each group): sham group, IRI group, low-dose dexmedetomidine group (LD group), high-dose dexmedetomidine group (HD group), combined low-dose dexmedetomidine and LY294002 group (LDL group), and combined high-dose dexmedetomidine and LY294002 group (HDL group). A 30-min ischemia was induced by occluding the hilum of the left lung, followed by a 120-min reperfusion by removing occlusion of the hilum. After the left lung was removed, the wet/dry weight ratio (W/D) of the lung tissues was determined. Pathological changes of lung tissues were evaluated by light and electron microscopes and the expression of p-Akt and HIF-1[alpha] in the lung tissues was determined by western blotting. Results Compared with the sham group, both the W/D ratio and lung injury were significantly increased, the p-Akt expression was down-regulated and HIF-1[alpha] expression was up-regulated in the five experimental groups. Compared with the LD and LDL groups, both the W/D ratio and lung injury were decreased, but the expression of p-Akt and HIF-1[alpha] was increased in the HD and HDL groups. Conclusions Administration of dexmedetomidine before ischemia can provide a protection against lung IRI by re-installing the PI3K/Akt/HIF-1[alpha] signaling pathway., Author(s): Wei Zhang[sup.1] , Jia-Qiang Zhang[sup.1] , Fan-Min Meng[sup.1] , Fu-Shan Xue[sup.2] Author Affiliations: (1) Department of Anesthesiology, Henan Provincial People's Hospital of Zhengzhou University, ZhengzhouPeople's Republic of China (2) [...]
- Published
- 2016
- Full Text
- View/download PDF