15 results on '"Blakley, Barry"'
Search Results
2. Effect of increasing concentration of ergot alkaloids in the diet of feedlot cattle: performance, welfare, and health parameters
- Author
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Sarich, Jenna M, primary, Stanford, Kim, additional, Schwartzkopf-Genswein, Karen S, additional, McAllister, Tim A, additional, Blakley, Barry R, additional, Penner, Gregory B, additional, and Ribeiro, Gabriel O, additional
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- 2023
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3. 156 Effect of Increasing Levels of Ergot Alkaloids in the Diet of Feedlot Cattle
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Sarich, Jenna M, primary, Stanford, Kim, additional, Schwartzkopf-Genswein, Karen S, additional, McAllister, Tim A, additional, Blakley, Barry R, additional, Penner, Gregory B, additional, and Ribeiro, Gabriel O, additional
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- 2022
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4. 152 Effect of Continuous or Intermittent Feeding of Ergot Contaminated Grain in a Mash or Pelleted Form on the Performance of Backgrounding Beef Steers
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Reynolds, Matthew R, primary, Stanford, Kim, additional, Meléndez, Daniela M, additional, Schwartzkopf-Genswein, Karen S, additional, McAllister, Timothy A, additional, Blakley, Barry R, additional, McKinnon, John J, additional, and Ribeiro, Gabriel O, additional
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- 2022
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5. Sustained vascular contractile response induced by an R- and S-epimer of the ergot alkaloid ergocristine and attenuation by a noncompetitive antagonist
- Author
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Cherewyk, Jensen E, primary, Parker, Sarah E, additional, Blakley, Barry R, additional, and Al-Dissi, Ahmad N, additional
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- 2022
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6. Effect of ergot alkaloids and a mycotoxin deactivating product on in vitro ruminal fermentation using the Rumen simulation technique (RUSITEC)
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Sarich, Jenna M, primary, Stanford, Kim, additional, Schwartzkopf-Genswein, Karen S, additional, Gruninger, Robert J, additional, McAllister, Tim A, additional, Meale, Sarah J, additional, Blakley, Barry R, additional, Penner, Gregory B, additional, and Ribeiro, Gabriel O, additional
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- 2022
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7. 150 Effect of Ergot Alkaloids and a Mycotoxin Deactivating Product on in vitro Rumen Fermentation Using the Rumen Simulation Technique (RUSITEC)
- Author
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Sarich, Jenna, primary, Stanford, Kim, additional, Schwartzkopf-Genswein, Karen S, additional, McAllister, Tim A, additional, Blakley, Barry, additional, Penner, Gregory B, additional, and Ribeiro, Gabriel O, additional
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- 2021
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8. Corrigendum to: Assessment of the vasoactive effects of the (S)-epimers of ergot alkaloids in vitro
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Cherewyk, Jensen E, primary, Parker, Sarah E, additional, Blakley, Barry R, additional, and Al-Dissi, Ahmad N, additional
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- 2021
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9. Assessment of the vasoactive effects of the (S)-epimers of ergot alkaloids in vitro
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Cherewyk, Jensen E, primary, Parker, Sarah E, additional, Blakley, Barry R, additional, and Al-Dissi, Ahmad N, additional
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- 2020
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10. Effect of Continuous Or Intermittent Feeding of Ergot Contaminated Grain in a Mash Or Pelleted Form on the Performance and Health Parameters of Finishing Beef Steers.
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Reynolds, Matthew R., Stanford, Kim, Meléndez, Daniela M., Schwartzkopf-Genswein, Karen S., McAllister, Tim A., Blakley, Barry R., McKinnon, John J., and Ribeiro, Gabriel O.
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ERGOT alkaloids ,BLOOD cell count ,PELLETED feed ,BLOOD urea nitrogen ,ALANINE aminotransferase ,FUSARIUM toxins ,HAPTOGLOBINS - Abstract
This study evaluated the effect of feeding pelleted or mash ergot contaminated grain fed continuously or intermittently on the growth performance, blood serum variables, and carcass quality of finishing beef steers. Sixty black angus steers (442 ± 36.7 kg) were used in a complete randomized finishing (90% concentrate:10% barley silage DM basis) 126-d study. Steers were randomly assigned to 4 different treatments and individually housed. Treatments included: 1) control ration [CON; no added ergot alkaloids (EA)], 2) continuous ergot mash (CEM; fed continuously at 2 ppm total EA), 3) intermittent ergot mash (IEM; fed at 2 ppm EA during the first week of each 21-d period and control diet for the remaining 2 weeks), and 4) intermittent ergot pellet (IEP; fed at 2 ppm EA incorporated into a pelleted supplement during the first week of each 21-d period and control diet for the other 2 weeks). Steers were weighed every 21 d and blood samples were collected on day 0, 42, 84, and 126. Steer DMI (P < 0.01, 9.95 vs. 11.05 kg/day) and ADG (P = 0.04) decreased for all EA treatments compared with the CON. Total shrunk BW gain (P = 0.03, 202.5 vs. 225.2 kg), final BW (P = 0.03, 617.9 vs. 662.2 kg), and carcass weight (P = 0.06) decreased for all EA steers compared with the CON steers. No treatment effects (P > 0.05) were observed for carcass dressing percentage, ribeye area, marbling score, or liver abscess. The percent of carcasses graded AAA decreased for all EA steers compared CON steers (P < 0.01, 46.7 vs. 93.3%). Steers fed EA had increased rectal temperature compared with CON steers (P < 0.01, 39.8 vs. 39.4 °C). Hair cortisol concentrations were less in steers fed IEP (P = 0.05, 1.23 vs. 1.76 pg/mg) than CON. No treatment effects (P > 0.05) were observed for complete blood count, and serum prolactin, haptoglobin, albumin, alkaline phosphatase, alanine transaminase, amylase, calcium, creatine, glucose, lipase, total bilirubin, total protein, or globulin. Blood urea nitrogen was less for EA steers compared with CON (P = 0.01, 3.48 vs. 4.10 mmol/L). The IEM steers had decreased concentrations of serum gamma-glutamyl transferase compared with IEP steers (P = 0.04, 23.4 vs. 28.5 IU). Pelleting ergot contaminated grain did not reduce the impact of ergot alkaloids on any of the measured parameters. Results suggest that continuously or intermittently feeding an ergot contaminated diet (2 ppm EA) can significantly reduce intake, growth performance, and carcass weight, with minimal impact on blood serum variables in finishing steers. Pelleting was not an effective method of reducing ergot toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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11. Effect of continuous or intermittent feeding of ergot contaminated grain in a mash or pelleted form on the performance and health of feedlot beef steers
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Reynolds, Matthew R, Stanford, Kim, Meléndez, Daniela M, Schwartzkopf-Genswein, Karen S, McAllister, Tim A, Blakley, Barry R, McKinnon, John J, and Ribeiro, Gabriel O
- Abstract
This study evaluated the effect of feeding ergot contaminated grain continuously or intermittently through backgrounding (BG) and finishing (FN) in a mash or pelleted supplement on the growth performance, health and welfare parameters, and carcass characteristics of feedlot beef steers. Sixty black Angus steers (300 ± 29.4 kg BW) were used in a complete randomized 238-d study. Steers were stratified by weight and randomly assigned to four different diets (15 steers/treatment) and individually housed. Treatments included: (1) control [CON; no added ergot alkaloids (EA)], (2) continuous ergot mash (CEM; fed continuously at 2 mg total EA/kg of DM), (3) intermittent ergot mash (IEM; fed at 2 mg total EA/kg of DM, during the first week of each 21-d period and CON for the remaining 2 wk, this feeding pattern was repeated in each period), and (4) intermittent ergot pellet (IEP; fed at 2 mg of total EA/kg of DM as a pellet during the first week of each 21-d period and CON for the remaining 2 wk as described for IEM). Steers were fed barley based BG diets containing 40% concentrate:60% silage (DM basis) for 84 d (four 21-d periods), transitioned over 28 d (no ergot fed) to an FN diet (90% concentrate:10% silage DM basis) and fed for 126 d (six 21-d periods) before slaughter. In the BG phase, steer DMI (P< 0.01, 7.45 vs. 8.05 kg/d) and ADG (P< 0.01) were reduced for all EA diets compared to CON. The CEM fed steers had lower ADG (P< 0.01, 0.735 vs. 0.980 kg) and shrunk final BW (P< 0.01, 350 vs. 366 kg) than CON. CEM had lower gain:feed (P< 0.07, 0.130 vs. 0.142) than CON. In the FN phase, steer DMI (P< 0.01, 9.95 vs. 11.05 kg/d) and ADG (P= 0.04) were also decreased for all EA fed steers compared to CON. Total shrunk BW gain (P = 0.03, 202.5 vs. 225.2 kg), final BW (P= 0.03, 617.9 vs. 662.2 kg), and carcass weight (P= 0.06) decreased for all EA fed steers compared to CON. The percentage of AAA carcasses decreased for all EA fed steers (P< 0.01, 46.7 vs. 93.3%) compared to CON. EA fed steers had increased rectal temperatures (P< 0.01, 39.8 vs. 39.4 °C) compared to CON. Pelleting ergot contaminated grain did not reduce the impact of ergot alkaloids on any of the measured parameters during BG or FN. Continuously or intermittently feeding ergot contaminated diets (2 mg total EA/kg of DM) significantly reduced intake, growth performance, and carcass weight, with minimal impact on blood parameters in feedlot steers. Pelleting was not an effective method of reducing ergot toxicity.This study evaluated the effect of continuous or intermittent feeding EA in a mash or pelleted form on growth performance, health and welfare parameters, and carcass characteristics of feedlot beef steers. Continuously or intermittently feeding an ergot contaminated diet (2 ppm EA) both reduced intake, growth performance, and carcass weight, but pelleting did not reduce the toxicities of EA.Produced by the fungus Claviceps purpurea, ergot alkaloids (EA) are toxic to beef cattle when consumed and can lead to reduction in feed intake and growth performance, vasoconstriction of the blood vessels, hyperthermia, damage to extremities (ears, tails, and hooves) and in severe cases, death. Grain is often cleaned to meet quality standards, and the resulting screenings are often utilized for feeding livestock and can have high concentrations of EA. The application of heat during pelleting of EA contaminated grain has been suggested to reduce its toxicity. Backgrounding and finishing beef cattle feeding experiments were conducted to assess the effect of continuously or intermittently feeding EA contaminated grain (2 mg/kg of diet DM) either as a pellet or as mash on growth performance, health, and animal welfare. Feeding EA grain continuously or intermittently either as a mash or pellet drastically reduced growth performance of steers, with no difference between treatments.
- Published
- 2024
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12. Effect of Continuous or Intermittent Feeding of Ergot Contaminated Grain in a Mash or Pelleted Form on the Performance of Backgrounding Beef Steers.
- Author
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Reynolds, Matthew R., Stanford, Kim, Meléndez, Daniela M., Schwartzkopf-Genswein, Karen S., McAllister, Timothy A., Blakley, Barry R., McKinnon, John J., and Ribeiro, Gabriel O.
- Subjects
CALVES ,GRAIN ,BLOOD cell count ,ERGOT alkaloids ,FEEDLOTS ,PELLETED feed - Abstract
The objective of this study was to evaluate the effect of feeding pelleted or mash ergot contaminated grain continuously or intermittently on performance and blood parameters of backgrounding steers. Sixty black angus steers (300 ± 29.4 kg) were used in a complete randomized backgrounding (60% barley silage:40% concentrate DM basis) 84 d study. Steers were randomly assigned to 4 different treatments and housed individually. Treatments included: 1) control ration (CON; no added ergot), 2) continuous ergot mash (CEM; fed continuously at 2 ppm), 3) intermittent ergot mash (IEM; fed at 2 ppm on the first wk of each 21 d period and control diet for the remaining 2 wk), and 4) intermittent ergot pellet (IEP; fed at 2 ppm during the first wk of each 21 d period and control diet for the other 2 wk). Calves were weighed every 21 days and blood samples were collected on d 0, 42, and 84. No treatment effects (P>0.05) were observed for complete blood count. Steer DMI was reduced for all ergot diets compared to the CON diet (P< 0.01, 7.45 vs. 8.05 kg/d). Steer ADG was reduced for all ergot diets compared to the CON diet (P< 0.01). CEM fed steers had lower ADG (P< 0.01, 0.980 vs. 0.735 kg) and shrunk final weight (P< 0.01, 366 vs. 350 kg) than CON calves, but CEM and IEM were no different. CEM fed steers had lower Gain:Feed (P< 0.07, 0.130 vs. 0.142) than CON calves. Pelleting ergot contaminated grain did not reduce the impact of ergot alkaloids on any of the measured parameters. Results suggest that continuously or intermittently feeding an ergot contaminated TMR (2 ppm) can significantly reduce the growth performance of backgrounded feedlot steers but no effects were observed for blood parameters. Pelleting was not an effective method of reducing ergot toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
13. Effect of Increasing Levels of Ergot Alkaloids in the Diet of Feedlot Cattle.
- Author
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Sarich, Jenna M., Stanford, Kim, Schwartzkopf-Genswein, Karen S., McAllister, Tim A., Blakley, Barry R., Penner, Gregory B., and Ribeiro, Gabriel O.
- Subjects
ERGOT alkaloids ,CATTLE nutrition ,BEEF cattle ,REDUCING diets ,WEIGHT gain ,SILAGE - Abstract
This study evaluated the effects of feeding ergot alkaloids (EA) at increasing levels (0, 0.75, 1.5, and 3.0 ppm) to feedlot beef cattle over backgrounding (BG) and finishing (FS) phases on growth performance and carcass quality. Steers (n=240; 280±32 kg) were blocked by weight and randomly allocated to 16 pens (4 pens/treatment; 15 steers/pen). Treatments included: no added EA (CTL); or EA added at 0.75 (EA075); 1.50 (EA150); and 3.0 ppm (EA300). Steers were fed barley-based BG diets containing 40% concentrate: 60% silage (DM basis) for 84 d, then stepped up over 28 d to a 90% concentrate:10% silage (DM basis) FS diet for 119 d. Steers fed EA300 received CTL in FS after 77 days on feed (DOF). In the BG phase, a quadratic decrease (P=0.05) in ADG and a linear decrease in final BW (P=0.03) and total BW gain (P=0.02) were observed with increasing EA. In the FS phase, up to 77 DOF, there was a linear decrease in steers DMI (P=0.04), ADG (P=0.01), and total BW gain (P=0.01) with increasing levels of EA. Over the full FS phase (119 DOF), a quadratic response was observed for ADG (P=0.05), final BW (P=0.05), total BW gain (P=0.02) and carcass weight (P=0.05) with steers fed EA150 showing the lowest performance. Gain:feed was not affected (P>0.20) by EA in the BG or FS phase. Dressing percentage (P=0.02) showed a quadratic effect with EA300 being the lowest. Additionally, a linear increase in lean meat yield (P< 0.001) was observed with increasing EA. Including EA in the diet promoted a linear increase in rectal temperature (P< 0.01). In conclusion, increasing levels of EA in the diet reduced growth performance, but when EA was removed, steers showed compensatory weight gain. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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14. Effect of ergot alkaloids and a mycotoxin deactivating product on in vitroruminal fermentation using the Rumen simulation technique (RUSITEC)
- Author
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Sarich, Jenna M, Stanford, Kim, Schwartzkopf-Genswein, Karen S, Gruninger, Robert J, McAllister, Tim A, Meale, Sarah J, Blakley, Barry R, Penner, Gregory B, and Ribeiro, Gabriel O
- Abstract
The rumen simulation technique (RUSITEC) was used to investigate the effect of ergot alkaloids (EA) and a mycotoxin deactivating product (Biomin AA; MDP) on nutrient digestion, ruminal fermentation parameters, total gas, methane, and microbial nitrogen production. Ruminal fermentation vessels received a feedlot finishing diet of 90:10 concentrate:barley silage (DM basis). Using a randomized complete block design, treatments were assigned (n= 4 vessels/treatment) within two RUSITEC apparatuses in a 2 × 2 factorial arrangement. Treatments included: (1) control (CON) diet (no EA and no MDP); (2) CON diet + 1 g/d MDP; (3) CON diet + 20 mg/kg EA; and (4) CON diet + 20 mg/kg EA + 1 g/d MDP. The study was conducted over 14 d with 7 d of adaptation and 7 d of sample collection. Data were analyzed in SAS using PROC MIXED including fixed effects of EA, MDP, and the EA×MDP interaction. Random effects included RUSITEC apparatus and cow rumen inoculum (n= 4). Ergot alkaloids decreased dry matter (DMD) (P= 0.01; 87.9 vs. 87.2%) and organic matter disappearance (OMD) (P= 0.02; 88.8 vs. 88.4%). Inclusion of MDP increased OMD (P= 0.01; 88.3 vs. 88.9%). Neutral detergent fiber disappearance (NDFD) was improved with MDP; however, an EA×MDP interaction was observed with MDP increasing (P< 0.001) NDFD more with EA diet compared to CON. Acetate proportion decreased (P= 0.01) and isovalerate increased (P= 0.03) with EA. Consequently, acetate:propionate was reduced (P= 0.03) with EA. Inclusion of MDP increased total volatile fatty acid (VFA) production (P< 0.001), and proportions of acetate (P= 0.03) and propionate (P= 0.03), and decreased valerate (P< 0.001), isovalerate (P= 0.04), and caproate (P= 0.002). Treatments did not affect (P≥ 0.17) ammonia, total gas, or methane production (mg/d or mg/g of organic matter fermented). The inclusion of MDP reduced (P< 0.001) microbial nitrogen (MN) production in the effluent and increased (P= 0.01) feed particle-bound MN. Consequently, total MN decreased (P= 0.001) with MDP. In all treatments, the dominant microbial phyla were Firmicutes, Bacteroidota, and Proteobacteria, and the major microbial genus was Prevotella. Inclusion of MDP further increased the abundance of Bacteroidota (P= 0.04) as it increased both Prevotella(P= 0.04) and Prevotellaceae_UCG-003 (P= 0.001). In conclusion, EA reduced OMD and acetate production due to impaired rumen function, these responses were successfully reversed by the addition of MDP.Ergot alkaloids can affect ruminal metabolism of cattle, which can have large impacts on production, welfare, and health. Mycotoxin deactivators can alleviate some of the negative impacts that dietary ergot has on cattle.Ergot formed from a parasitic fungus (Claviceps purpurea) affects various types of grains (rye, wheat, or oats) and may contain several toxic ergot alkaloids (EA). Individual EA may impact the rumen microorganisms, and cattle feed intake, digestibility, health, and overall performance. A common method to alleviate toxicity in mycotoxin-contaminated feed is through the addition of mycotoxin binders (MDP); however, their efficacy against EA is unknown. To better understand the effect of EA in cattle, we performed an in vitroexperiment to examine the impact of EA on the ruminal microbial populations and fermentation of a finishing feedlot diet using an artificial rumen (RUSITEC). Additionally, an MDP was added to test if it could reduce the detrimental effects of EA on rumen fermentation. MDP increased total volatile fatty acids (VFA) and reduced total microbial protein synthesis. Furthermore, EA reduced microbial diversity and the acetate:propionate ratio. Although EA reduced organic matter digestibility and acetate production, these negative effects were reversed by the addition of the MDP.
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- 2022
- Full Text
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15. Effect of Ergot Alkaloids and a Mycotoxin Deactivating Product on in vitro Rumen Fermentation Using the Rumen Simulation Technique (RUSITEC).
- Author
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Sarich, Jenna, Stanford, Kim, Schwartzkopf-Genswein, Karen S., McAllister, Tim A., Blakley, Barry, Penner, Gregory B., and Ribeiro, Gabriel O.
- Subjects
ERGOT alkaloids ,RUMEN fermentation ,SIMULATION methods & models ,FATTY acids ,ORGANIC compounds ,METHANE ,AMMONIA ,MYCOTOXINS - Abstract
A rumen simulation technique (RUSITEC) investigated effects of ergot alkaloids (E) and a mycotoxin deactivating product (Biomin® AA; B) on nutrient digestion, rumen fermentation, total gas and methane emissions. Fermenters received a finishing diet of 90:10 concentrate:barley silage. Using a randomized complete block design, treatments were assigned (4 vessels/treatment) within two RUSITECs in a 2 × 2 factorial. Treatments included: 1) control diet, 2) control + 1 g B, 3) control + 20 ppm E, and 4) control + 20 ppm E + 1 g B. The study had a 14-d experimental period, with 7-d adaptation and 7-d sample collection. Data were analyzed in SAS using PROC mixed including fixed effects of E, B, and E×B interaction. Random effects included RUSITEC apparatus and cow rumen inoculum (n = 4). Ergot decreased dry matter disappearance (DMD) (P < 0.05; 87.9 vs. 87.2%) and organic matter disappearance (OMD) (P < 0.05; 88.8 vs. 88.4%). Adding B increased OMD (P < 0.05; 88.3 vs. 88.9%) and neutral detergent fiber disappearance (NDFD) (P < 0.01); however, an E×B interaction was observed for NDFD (P < 0.01) with B promoting greater increases with E. Ergot decreased acetate proportions (P < 0.01) and increased isovalerate (P < 0.05). Consequently, acetate:propionate was reduced (P < 0.05) with E. Inclusion of B increased total volatile fatty acids (P < 0.01), and proportions of acetate (P < 0.05) and propionate (P < 0.05), and decreased valerate (P < 0.01), isovalerate (P < 0.01), and caproate (P < 0.01). Treatments did not affect (P = 0.17) ammonia-N, total gas, or methane production (mg/d or mg/g of OM fermented). In conclusion, E reduced OMD and acetate production, but these responses were reversed by the addition of B. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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