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The objective of this study was to determine the effect on digestibility of forages using a daisy jar system with inoculum from steers supplemented with the yeast fermentation product Saccharomyces cerevisiae. Forages tested included ryegrass, wheat, orchard grass, alfalfa, and sorghum sudan hays. Samples were ground through a 1mm screen, placed in Ankom F57 bags, and run in triplicate to increase statistical significance. Two ruminally cannulated jersey steers were used in a switchback design. Animals were housed in a dry lot pen. The study consisted of 2 three-week periods, 2-week standardization followed by a 1-week test period. Diets offered for period 1 were steer A = 100% alfalfa hay w/o yeast product and steer B = 100% wheat hay w/o yeast product. Diets offered for period 2 were steer A =100% alfalfa hay +15g/h/d yeast product and steer B =100% wheat hay +15g/h/d yeast product. During the test week, rumen fluid contents were collected from each donor via rumen cannula approximately 4 h after feeding and taken to the lab to be composited. The samples were incubated for 0 h, 24 h, 48 h, 72 h, and 96 h. At the end of the incubation period bags were collected and cold shocked to stop microbial digestion. Samples were dried at 55oC and stored for later analysis. Model included trt (+/-yeast), forage, hr, and interactions forage*hr and forage*trt. No significant differences were observed (P = 0.38) on DM digestibility or NDF digestibility (P = 0.76) with the supplementation of yeast fermentation product. Forage*Trt was significant (P = 0.03 DMdig, P = 0.02 NDFdig). Implications of this study are the inclusion of a Saccharomyces cerevisiae fermentation product at a rate of 15g/h/d may not impact overall forage NDF or DM digestibility but may impact types of forage differently in an in vitro system using composited inoculum from donors on a forage diet. [ABSTRACT FROM AUTHOR]

Enhanced microbial activity in the equine hindgut increases nutrient availability, reduces fecal waste, and adds value to low quality forages. The dietary supplement DigestaWell® Fiber (DF; fibrolytic cofactors: methionine and B vitamins), designed to increase microbial efficiency, has been shown to improve in-vitro digestibility of alfalfa and tall fescue forages after 48h incubation. The objective of this study was to determine if DF would improve in-vitro dry matter digestibility (DMD) and ADF digestibility (ADFD) of alfalfa and coastal bermudagrass hays incubated for 48h or 72h. Utilizing a crossover design, four mature Quarter Horse mares were fed 2% BW daily of alfalfa (AF; 32.8% NDF, 27.1% ADF) or coastal bermudagrass (CB; 63.2% NDF, 35.4% ADF) hay for 19d periods. DMD and ADFD were determined after 48h or 72h incubation with or without treatment (320mg/L DF) at 37.5°C in an ANKOM Daisy II incubator inoculated with 200g fresh feces. Forage samples were run in triplicate. Data were transformed to approximate normality, then analyzed using a repeated measures mixed model in SAS with main effects of forage, treatment, incubation time, and feeding period, and statistically significant interactions. Across incubation times, DMD was higher for AF than CB (P < 0.01; 49.4% vs 42.1%). Across forages, DMD was higher at 72h than at 48h incubation (P < 0.01; 47.5% vs 43.9%). DF improved DMD across forages at 48h (P = 0.03), but not at 72h (P = 0.47). Across incubation times, DF improved DMD of CB (P < 0.01), but not AF (P = 0.79). Additionally, across both forages and both incubation times, DF improved ADFD (P < 0.01). In conclusion, DF may improve microbial fermentation in the equine hindgut. Future research should investigate in-vivo effects of oral supplementation of DF on forage digestibility measures. [ABSTRACT FROM AUTHOR]

The purpose of this study was to determine if supplementation of a yeast fermentation product had an effect on the ADG, performance, and meat quality characteristics of Kathadin sheep fed a ryegrass hay based diet in drylot. Twenty-four Katahdin lambs were divided into two groups (male, n = 8; female n = 16) based on weight and gender: a control (CON) and treatment (TRT) used in a repeated block design. The lambs had a BW of 21.5 ± 2.5 kg, and concluded with a finishing weight of 36.3 ± 3.4 kg. Lambs were housed on dirt drylot with shade structures, and offered an ad libitum TMR diet was formulated to be isocaloric and isonitrogenous at 14% CP and was ryegrass hay based, and top dressed with ground corn as a carrier. The TRT received the yeast supplement at a rate of 4/g/h/d. The diet was offered at 2% of the group's body weight ad libitum. A 1-wk standardization period was conducted before the start of the study to obtain a 5% refusal rate. Orts were collected and recorded, as well as weekly feed samples were collected twice a week, and compiled for a chemical analysis for NDF, ADF, ASH, CP, and ether extract. Temperature and humidity data was also collected throughout the study. Lambs were weighed weekly with a rolling weight being used for the ADG of the lambs. Proc mixed procedures of SAS was used for data analysis. We found no statistical significance in DMI (CON=11.14 ±3.36 kg/d and TRT= 10.89 ±3.55 kg/d per group), and no statistical significance by gender (P < 0.11). There was a statistical significance (P < 0.03), for ADG per group. This suggests that there is a tendency for greater performance and ADG for lambs supplemented with a yeast fermentation product. [ABSTRACT FROM AUTHOR]

The purpose of this study was to determine if supplementation of a yeast fermentation product had an effect on the ADG, performance, and meat quality characteristics of Katahdin sheep fed a ryegrass hay based diet in drylot. Twenty-four Katahdin lambs were divided into two groups (male, n = 8; female n = 16) based on weight and gender: a control (CON) and treatment (TRT) used in a repeated block design. The lambs had a BW of 21.5 ± 2.5 kg, and concluded with a finishing weight of 36.3 ± 3.4 kg. Lambs were housed on dirt drylot with shade structures, and offered an ad libitum TMR diet was formulated to be isocaloric and isonitrogenous at 14% CP and was ryegrass hay based, and top dressed with ground corn as a carrier. The TRT received the yeast supplement at a rate of 4/g/h/d. The diet was offered at 2% of the group's body weight ad libitum. A 1-wk standardization period was conducted before the start of the study to obtain a 5% refusal rate. Orts were collected and recorded, as well as weekly feed samples were collected twice a week, and compiled for a chemical analysis for NDF, ADF, ASH, CP, and ether extract. Temperature and humidity data was also collected throughout the study. Lambs were weighed weekly with a rolling weight being used for the ADG of the lambs. Proc mixed procedures of SAS was used for data analysis. We found no statistical significance in DMI (CON=11.14 ±3.36 kg/d and TRT= 10.89 ±3.55 kg/d per group), and no statistical significance by gender (P < 0.11). There was a statistical significance (P < 0.03), for ADG per group. This suggests that there is a tendency for greater performance and ADG for lambs supplemented with a yeast fermentation product. [ABSTRACT FROM AUTHOR]

Enhanced microbial activity in the equine hindgut increases nutrient availability, reduces fecal waste, and adds value to low quality forages. The dietary supplement DigestaWell® Fiber (DF; fibrolytic cofactors: methionine and B vitamins), designed to increase microbial efficiency, has been shown to improve in-vitro digestibility of alfalfa and tall fescue forages after 48-h incubation. The objective of this study was to determine if DF would improve in-vitro dry matter digestibility (DMD) and ADF digestibility (ADFD) of alfalfa and coastal bermudagrass hays incubated for 48h or 72h. Utilizing a crossover design, four mature Quarter Horse mares were fed 2% BW daily of alfalfa (AF; 32.8% NDF, 27.1% ADF) or coastal bermudagrass (CB; 63.2% NDF, 35.4% ADF) hay for 19-d periods. DMD and ADFD were determined after 48-h or 72-h incubation with or without treatment (320mg/L DF) at 37.5°C in an ANKOM Daisy II incubator inoculated with 200g fresh feces. Forage samples were run in triplicate. Data were transformed to approximate normality, then analyzed using a repeated measures mixed model in SAS with main effects of forage, treatment, incubation time, and feeding period, and statistically significant interactions. Across incubation times, DMD was higher for AF than CB (P < 0.01; 49.4% vs 42.1%). Across forages, DMD was higher at 72h than at 48h incubation (P < 0.01; 47.5% vs 43.9%). DF improved DMD across forages at 48h (P = 0.03), but not at 72h (P = 0.47). Across incubation times, DF improved DMD of CB (P < 0.01), but not AF (P = 0.79). Additionally, across both forages and both incubation times, DF improved ADFD (P < 0.01). In conclusion, DF may improve microbial fermentation in the equine hindgut. Future research should investigate in-vivo effects of oral supplementation of DF on forage digestibility measures. [ABSTRACT FROM AUTHOR]