1. Sperabillins, new antibacterial antibiotics with potent in vivo activity. Taxonomy, fermentation, isolation and biological activity.
- Author
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Katayama N, Nozaki Y, Tsubotani S, Kondo M, Harada S, and Ono H
- Subjects
- Amidines chemistry, Amidines isolation & purification, Amidines pharmacology, Animals, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification, Anti-Bacterial Agents pharmacology, Bacterial Infections drug therapy, Bacterial Proteins biosynthesis, Bacterial Proteins drug effects, Cell Membrane drug effects, Cell Membrane metabolism, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Culture Media, DNA, Bacterial biosynthesis, DNA, Bacterial drug effects, Fermentation, Magnetic Resonance Spectroscopy, Mice, Peptidoglycan biosynthesis, Peptidoglycan drug effects, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa metabolism, Pseudomonas fluorescens classification, RNA, Bacterial biosynthesis, RNA, Bacterial drug effects, Spectrophotometry, Infrared, Spectrophotometry, Ultraviolet, Staphylococcus aureus genetics, Staphylococcus aureus metabolism, Anti-Bacterial Agents biosynthesis, Bacteria drug effects, Pseudomonas aeruginosa drug effects, Pseudomonas fluorescens metabolism, Staphylococcus aureus drug effects
- Abstract
A Gram-negative bacterium was found to produce new antibacterial antibiotics, sperabillins A, B, C and D, and the producing bacterium was characterized and identified as Pseudomonas fluorescens YK-437. Sperabillins were isolated by column chromatographies using cation-exchange resins, activated carbon and cation-exchange Sephadex, and preparative reverse-phase HPLC. Sperabillins showed antibacterial activity against Gram-negative and Gram-positive bacteria including antibiotic-resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. Sperabillin A inhibited DNA, RNA, protein, and cell wall biosynthesis in Escherichia coli. Sperabillins showed good protective effects in experimentally infected mice.
- Published
- 1992
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