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Present study was conducted to investigate the post parturient behavioural activities of Surti buffaloes (n = 25) and their calves using low light-intensity cameras and digital video recorder at five stages (I: from calving to half an hour, II: 6-6:30 hours, III: 12-12:30 hours, IV: 18-18:30 hours and V: 24-24:30 hours after calving) over a period of 24 h immediately after calving. Based on their maternal behaviour score (MBS) dams were categorized into two classes that is, group 1 (MBS = 1: very aggressive and 2: very attentive) and group 2 (MBS = 3: indifferent and 4: apathetic) where dam scored MBS 1 represented very aggressive and 4 apathetic. Overall eating time was significantly (P < .05) higher in buffalo cows of group 2 (8.87 ± 1.34 min) than in group 1 (5.37 ± 0.50 min). Dams of both the groups spent significantly (P < .05) longer time in sniffing and licking calf's body and licking ano-genital area of calf during stage I than later stages. Significantly (P < .05) longer time intervals from birth to standing (53.03 ± 3.04 min) were observed in group 2 calves. A positive correlation (P < .01) was observed between the time interval from birth to suckling and duration of active teat seeking (0.525). It may be inferred that post parturient neonatal behaviour have certain level of relationship with maternal behaviour score, whereas, parity and sex did not show any significant effect on MBS in Surti buffaloes. [ABSTRACT FROM AUTHOR]

Escherichia coli are important pathogens among bacterial causes of neonatal piglet diarrhoea. Their adhesion to the intestinal epithelial cells is an essential prerequisite for incidence of diarrhoea, which makes certain animal innately resistant and this adhesion pattern is genetically controlled. Among the candidate genes associated with adhesion pattern, MUC13 is the most likely responsible gene examined in diverse outbred swine populations. The present investigation aimed to evaluate the native Indian (desi) pigs in terms of E. coli adhesion pattern (with Indian isolate) and MUC13 expression profile across adhesion phenotypes. Out of 80 pigs screened, 27 were found to be non-adhesive, 46 adhesive and 7 were weakly adhesive. Effect of sex was found to be significant (P<.05) with non-significant age effect. RT-PCR analysis revealed that although, porcine MUC13 mRNA expression was highest in adhesive (2.67±0.69 fold), moderate in weakly adhesive (1.22±0.35 fold) and low levels in non-adhesive (calibrator) phenotypes, differences were statistically non-significant (P<.05). While the availability of non-adhesive phenotypes among Indian desi pigs implicates abundance of input genetic resources to start a breeding programme against diarrhoea, the descending expression of MUC 13 in adhesive and weakly adhesive samples speculates its important role in adhesion of E. coli. [ABSTRACT FROM AUTHOR]

In present investigation genetic variability in humoral response to classical swine fever (CSF) vaccination was explored. The aim of the study was to find association of 14 single nucleotide polymorphisms (SNPs) (IL-1β, MX1, IRF3 and SLA genes) with humoral response measured in terms of percentage inhibition of E2 antibody by competitive enzyme-linked immunosorbent assay. The post-vaccination response of CSF vaccination was low to moderate inDesipiglets ranged from 25.29% to 92.23% whereas it was moderate to high inDesi×Landracepiglets ranged from 69.30% to 100%. The response to CSF vaccination was significantly (P< 0.0001) differing inDesiandDesi×Landracepiglets. The mean percentage inhibition inDesipiglets was 62.73% but inDesi×Landracecross-bred it was 97.24%. None of the SNPs of present investigation was significantly associated with humoral immune response inDesipiglets. Two SNPs namely rs80937718 (SLA DQA1) and rs80929898 (SLA DQA1) were significantly (P< 0.01) affecting the humoral response of CSF vaccination inDesi×Landracepiglets. [ABSTRACT FROM PUBLISHER]

In the present study, polymorphisms at two single nucleotide polymorphisms (SNPs)  and one microsatellite locus of SLC11A1 gene were investigated for finding their association with susceptibility to bovine tuberculosis trait (tuberculin reaction) in Indian cattle. A total of 245 animals were tested with single intradermal tuberculin test for screening positive animals for tuberculin reaction. At rs109915208 locus very low polymorphism information content of 0.0454 was observed, whereas it was moderate (0.352) at rs109453173 locus of SLC11A1. At rs109915208 locus the genotypic as well as allelic frequencies were differing significantly (p-value < .05) in case–control animals where the odds ratio (OR) of ‘CC’verses‘CT’ genotype and the OR of ‘C’verses ‘T’ allele were approaching towards infinity, suggesting that animals having ‘CT’ genotype and ‘T’ allele were less susceptible for tuberculin reaction as compared to their contemporary genotype/allele. The significantly associated SNP was non-synonymous causing an amino acid change. [ABSTRACT FROM PUBLISHER]