1. Selection of cell wall antigens for the rapid detection of bacteria by immunological methods.
- Author
-
Labadie J and Desnier I
- Subjects
- Animals, Antigens, Surface analysis, Bacterial Outer Membrane Proteins analysis, Bacterial Outer Membrane Proteins immunology, Bacterial Outer Membrane Proteins isolation & purification, Blotting, Western, Cattle, Cell Wall immunology, Cross Reactions, Enterobacteriaceae immunology, Enterobacteriaceae isolation & purification, Enterobacteriaceae ultrastructure, Enzyme-Linked Immunosorbent Assay, Food Preservation, Gram-Negative Bacteria isolation & purification, Gram-Negative Bacteria ultrastructure, Gram-Positive Bacteria isolation & purification, Gram-Positive Bacteria ultrastructure, Lactobacillus immunology, Lactobacillus isolation & purification, Lactobacillus ultrastructure, Pseudomonas immunology, Pseudomonas isolation & purification, Pseudomonas ultrastructure, Swine, Turkeys, Antigens, Bacterial analysis, Food Microbiology, Gram-Negative Bacteria immunology, Gram-Positive Bacteria immunology, Meat
- Abstract
Some proteins extracted from the cell wall of Gram-positive and Gram-negative bacteria that affect the time for which meat can be stored are antigens produced by the strains from our laboratory collection or from field samples. Moreover, the rapid detection of bacteria (within 8 h for Gram-positive or 5 h for Gram-negative) that influence the quality of meat is made possible by immunochemical techniques such as ELISA or by introducing simultaneous detection of these antigens, as no cross-reactions have been observed.
- Published
- 1992
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