1. Fabrication of a tunable hydrogel membrane for constructing indirect cell coculture system
- Author
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Xiaojun Ma, Weiting Yu, Yizhe Song, Xiuli Wang, Na Li, Yan Lv, Guoshuang Zheng, and Demeng Zhang
- Subjects
0301 basic medicine ,Materials science ,Fabrication ,Polymers and Plastics ,Cell ,Nanotechnology ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,Polyelectrolyte ,Surfaces, Coatings and Films ,Chitosan ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Membrane ,medicine.anatomical_structure ,chemistry ,Permeability (electromagnetism) ,Materials Chemistry ,medicine ,Biophysics ,Hydrogel membrane ,0210 nano-technology ,Intracellular - Abstract
In this study, an improved indirect cell coculture system was constructed by using a polyelectrolyte complex membrane generated by alginate (A) and chitosan (C). Methodologies of characterizing thickness and permeability of flat AC membrane were first established due to the importance of these two parameters in determining intercellular distance and degree of contact between cocultured cells. Compared to reaction time, both alginate concentration and molecular weight (Mw) of chitosan play more dominant roles in determining the membrane thickness and diffusion coefficients. Moreover, cells in the alginate gel and on the AC membrane could maintain high cell viability. Thus, an improved indirect cell coculture system constructed by flat AC membrane was fabricated and characterized, which provides a robust tool to study the effect of intercellular distance and degree of contact between cocultured cells on cell–cell interactions. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43100.
- Published
- 2015
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