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The ability to estimate the rate of dissipation ( ε) of turbulent kinetic energy at middepth in a high-speed tidal channel using broadband acoustic Doppler current profilers (ADCPs) is assessed by making comparisons to direct measurements of ε obtained using shear probes mounted on a streamlined underwater buoy. The investigation was carried out in Grand Passage, Nova Scotia, Canada, where the depth-averaged flow speed reached 2 m s−1 and the Reynolds number was 8 × 107. The speed bin-averaged dissipation rates estimated from the ADCP data agree with the shear probe data to within a factor of 2. Both the ADCP and the shear probe measurements indicate a linear dependence of ε on the cube of the flow speed during flood and much lower dissipation rates during ebb. The ebb-flood asymmetry and the small-scale intermittency in ε are also apparent in the lognormal distributions of the shear probe data. Possible sources of bias and error in the ε estimates are investigated, and the most likely causes of the discrepancy between the ADCP and shear probe estimates are the cross-channel separation of the instruments and the high degree of spatial variability that exists in the channel. [ABSTRACT FROM AUTHOR]