1. Role of MOG-stimulated Th1 type 'light up' (GFP+) CD4+ T cells for the development of experimental autoimmune encephalomyelitis (EAE)
- Author
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Takehiro Koshio, Yoshikazu Yuki, Mamoru Yura, Hiroshi Kiyono, Ichiro Takahashi, Keiji Nakagami, and Masashi Serada
- Subjects
Adoptive cell transfer ,Encephalomyelitis, Autoimmune, Experimental ,Encephalomyelitis ,T cell ,Immunology ,Green Fluorescent Proteins ,Molecular Sequence Data ,Lymphocyte Activation ,Myelin oligodendrocyte glycoprotein ,Mice ,Immune system ,Antigen ,immune system diseases ,Cell Movement ,T-Lymphocyte Subsets ,medicine ,Immunology and Allergy ,Animals ,Amino Acid Sequence ,biology ,Experimental autoimmune encephalomyelitis ,Brain ,T lymphocyte ,Th1 Cells ,medicine.disease ,Molecular biology ,Adoptive Transfer ,Peptide Fragments ,Mice, Inbred C57BL ,Luminescent Proteins ,Myelin-Associated Glycoprotein ,medicine.anatomical_structure ,Spinal Cord ,Antigens, Surface ,biology.protein ,Cytokines ,Female ,Myelin-Oligodendrocyte Glycoprotein ,Myelin Proteins - Abstract
Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis in humans. EAE can be passively transferred into naive syngeneic animals by administration of MOG-specific T cell clones. Lymphocytes isolated from green fluorescent protein (GFP)-transgenic (Tg) mice can light up by emitting green fluorescence, thus making it feasible to use such animals in a passive transfer model for EAE. When MOG-sensitized splenic lymphocytes from GFP-Tg mice were adoptively transferred to irradiated, syngeneic C57BL/6 and RAG-1(-/-)mice, typical symptoms of EAE developed. Analysis of the reconstituted mice with EAE revealed prominent infiltration of fluorescing (GFP+), CD4+ T cells into the central nervous system (CNS). Real-time confocal imaging revealed these cells in the spinal cords and brains of recipient mice. This infiltration was also confirmed by anti-GFP monoclonal antibodies. Furthermore, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) evaluation indicated that the infiltrating GFP+, CD4+ T cells exclusively produced T helper type 1 (Th1) cytokines, especially interferon-gamma (IFN-gamma). These results clearly show that MOG-specific CD4+ T cells preferentially invade into the CNS and mediate the development of EAE by producing Th1-biased cytokines.
- Published
- 2001