10 results on '"Baker, Stephen"'
Search Results
2. Molecular characterization of the Salmonella enterica Serovar Typhi Vi-typing bacteriophage E1
- Author
-
Pickard, Derek, Thomson, Nicholas R., Baker, Stephen, Wain, John, Pardo, Mercedes, Goulding, David, Hamlin, Nancy, Choudhary, Jyoti, Threfall, John, and Dougan, Gordon
- Subjects
Salmonella -- Genetic aspects ,Biochemical genetics -- Research ,Virulence (Microbiology) -- Evaluation ,Bacteriophages -- Identification and classification ,Biological sciences - Abstract
Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated El-typing bacteriophage using a number of molecular approaches. The absolute requirement for Vi capsule expression for infectivity was demonstrated using different Vi-negative S. enterica derivatives. The phage particles were shown to have an icosahedral head and a long noncontractile tail structure. The genome is 45,362 bp in length with defined capsid and tail regions that exhibit significant homology to the S. enterica transducing phage ES18. Mass spectrometry was used to confirm the presence of a number of hypothetical proteins in the Vi phage El particle and demonstrate that a number of phage proteins are modified posttranslationally. The genome of the Vi phage E1 is significantly related to other bacteriophages belonging to the same serovar Typhi phage-typing set, and we demonstrate a role for phage DNA modification in determining host specificity.
- Published
- 2008
3. Analysis of the hypervariable region of the Salmonella enterica genome associated with [tRNA.sup.leuX]
- Author
-
Bishop, Anne L., Baker, Stephen, Jenks, Sara, Fookes, Maria, Gaora, Peadar O., Pickard, Derek, Anjum, Muna, Farrar, Jeremy, Hien, Tran T., Ivens, Al, and Dougan, Gordon
- Subjects
Escherichia coli -- Research ,Escherichia coli -- Genetic aspects ,Bacterial genetics -- Research ,Bacteriology -- Research ,Salmonella -- Research ,Salmonella -- Genetic aspects ,Biological sciences - Abstract
The divergence of Salmonella enterica and Escherichia coli is estimated to have occurred approximately 140 million years ago. Despite this evolutionary distance, the genomes of these two species still share extensive synteny and homology. However, there are significant differences between the two species in terms of genes putatively acquired via various horizontal transfer events. Here we report on the composition and distribution across the Salmonella genus of a chromosomal region designated SPI-10 in Salmonella enterica serovar Typhi and located adjacent to [tRNA.sup.leuX]. We find that across the Salmonella genus the [tRNA.sup.leuX] region is a hypervariable hot spot for horizontal gene transfer; different isolates from the same S. enterica serovar can exhibit significant variation in this region. Many P4 phage, plasmid, and transposable element-associated genes are found adjacent to [tRNA.sup.leuX] in both Salmonella and E. coil, suggesting that these mobile genetic elements have played a major role in driving the variability of this region.
- Published
- 2005
4. Genomic and genetic analysis of Bordetella bacteriophages encoding reverse transcriptase-mediated tropism-switching cassettes
- Author
-
Liu, Minghsun, Gingery, Mari, Doulatov, Sergei R., Liu, Yichin, Hodes, Asher, Baker, Stephen, Davis, Paul, Simmonds, Mark, Churcher, Carol, Mungall, Karen, Quail, Michael A., Preston, Andrew, Harvill, Eric T., Maskell, Duncan J., Eiserling, Frederick A., Parkhill, Julian, and Miller, Jeff F.
- Subjects
Biological sciences - Abstract
Liu et al. recently described a group of related temperate bacteriophages that infect Bordetella subspecies and undergo a unique template-dependent, reverse transcriptase-mediated tropism switching phenomenon (Liu et al., Science 295:2091-2094, 2002). Tropism switching results from the introduction of single nucleotide substitutions at defined locations in the VR1 (variable region 1) segment of the mtd (major tropism determinant) gene, which determines specificity for receptors on host bacteria. In this report, we describe the complete nucleotide sequences of the 42.5- to 42.7-kb double-stranded DNA genomes of three related phage isolates and characterize two additional regions of variability. Forty-nine coding sequences were identified. Of these coding sequences, bbp36 contained VR2 (variable region 2), which is highly dynamic and consists of a variable number of identical 19-bp repeats separated by one of three 5-bp spacers, and bpm encodes a DNA adenine methylase with unusual site specificity and a homopolymer tract that functions as a hotspot for frameshift mutations. Morphological and sequence analysis suggests that these Bordetella phage are genetic hybrids of P22 and T7 family genomes, lending further support to the idea that regions encoding protein domains, single genes, or blocks of genes are readily exchanged between bacterial and phage genomes. Bordetella bacteriophages are capable of transducing genetic markers in vitro, and by using animal models, we demonstrated that lysogenic conversion can take place in the mouse respiratory tract during infection.
- Published
- 2004
5. Composition, acquisition, and distribution of the Vi exopolysaccharide-encoding Salmonella enterica pathogenicity island SPI-7
- Author
-
Pickard, Derek, Wain, John, Baker, Stephen, Line, Alexandra, Chohan, Sonia, Fookes, Maria, Barron, Andrew, Gaora, Peadar O., Chabalgoity, Jose A., Thanky, Niren, Scholes, Christoph, Thomson, Nicholas, Quail, Michael, Parkhill, Julian, and Dougan, Gordon
- Subjects
DNA -- Genetic aspects ,RNA -- Genetic aspects ,Gene expression -- Physiological aspects ,Salmonella typhimurium -- Genetic aspects ,Polysaccharides -- Physiological aspects ,Bacteriology -- Research ,Biological sciences - Abstract
Vi capsular polysaccharide production is encoded by the viaB locus, which has a limited distribution in Salmonella enterica serovars. In S. enterica serovar Typhi, viaB is encoded on a 134-kb pathogenicity island known as SPI-7 that is located between partially duplicated tRN[A.sup.pheU] sites. Functional and bioinformatic analysis suggests that SPI-7 has a mosaic structure and may have evolved as a consequence of several independent insertion events. Analysis of viaB-associated DNA in Vi-positive S. enterica serovar Paratyphi C and S. enterica serovar Dublin isolates revealed the presence of similar SPI-7 islands. In S. enterica serovars Paratyphi C and Dublin, the SopE bacteriophage and a 15-kb fragment adjacent to the intact tRN[A.sup.pheU] site were absent. In S. enterica serovar Paratyphi C only, a region encoding a type IV pilus involved in the adherence of S. enterica serovar Typhi to host cells was missing. The remainder of the SPI-7 islands investigated exhibited over 99% DNA sequence identity in the three serovars. Of 30 other Salmonella serovars examined, 24 contained no insertions at the equivalent tRN[A.sup.pheU] site, 2 had a 3.7-kb insertion, and 4 showed sequence variation at the tRN[A.sup.pheU]phoN junction, which was not analyzed further. Sequence analysis of the SPI-7 region from S. enterica serovar Typhi strain CT18 revealed significant synteny with clusters of genes from a variety of saprophytic bacteria and phytobacteria, including Pseudomonas aeruginosa and Xanthomonas axonopodis pv. citri. This analysis suggested that SPI-7 may be a mobile element, such as a conjugative transposon or an integrated plasmid remnant.
- Published
- 2003
6. Yersinia pestis pFra shows biovar-specific differences and recent common ancestry with a Salmonella enterica serovar Typhi plasmid
- Author
-
Prentice, Michael B., James, Keith D., Parkhill, Julian, Baker, Stephen G., Stevens, Kim, Simmonds, Mark N., Mungall, Karen L., Churcher, Carol, Oyston, Petra C., Titball, Richard W., Wren, Brendan W., Wain, John, Pickard, Derek, Hien, Tran Tinh, Farrar, Jeremy J., and Dougan, Gordon
- Subjects
Yersinia pestis -- Genetic aspects ,Population genetics -- Analysis ,Plasmids -- Genetic aspects ,Biological diversity -- Analysis ,Biological sciences - Abstract
Results reveal that more than 50% of the Yersinia pestis pMT1/pFra plasmid and a cryptic plasmid of Salmonella enterica serovar Typhi exhibit a common ancestry. Data support that Y. pestis biovar Orientalis emerged from biovars Medievalis and antiqua and specific plasmid rearrangements in P. pestis distinguish Orientalis from other biovars.
- Published
- 2001
7. Genomic comparison of Salmonella enterica serovars and Salmonela bongori by use of an S. enterica serovar Typhimurium DNA microarray
- Author
-
Chan, Kaman, Baker, Stephen, Kim, Charles C., Detweiler, Corrella S., Dougan, Gordon, and Falkow, Stanley
- Subjects
Bacteriology -- Research ,Salmonella typhimurium -- Genetic aspects ,Salmonella typhimurium -- Physiological aspects ,DNA microarrays -- Genetic aspects ,DNA microarrays -- Physiological aspects ,Genomes -- Physiological aspects ,Hybridization -- Genetic aspects ,Biological sciences - Abstract
Researchbhas been conducted on the genus Salmonella consisting of serovars which are different in their host range. The genomic hybridizations of these serovars and strains of S. enterica and S. bongori have been performed via the use of S. enterica serovar Typhimurium spotted DNA microarray in investigating serovars' genetic organization, and the results are reported.
- Published
- 2003
8. Analysis of the Hypervariable Region of the Salmonella enterica Genome Associated with tRNAleuX.
- Author
-
Bishop, Anne L., Baker, Stephen, Jenks, Sara, Fookes, Maria, Gaora, Peadar Ó., Pickard, Derek, Anjum, Muna, Farrar, Jeremy, Hien, Tran T., Ivens, Al, and Dougan, Gordon
- Subjects
- *
SALMONELLA , *ESCHERICHIA coli , *ENTEROBACTERIACEAE , *BACTERIAL genetics , *BACTERIOLOGY , *BACTERIAL genomes - Abstract
The divergence of Salmonella enterica and Escherichia coli is estimated to have occurred approximately 140 million years ago. Despite this evolutionary distance, the genomes of these two species still share extensive synteny and homology. However, there are significant differences between the two species in terms of genes putatively acquired via various horizontal transfer events. Here we report on the composition and distribution across the Salmonella genus of a chromosomal region designated SPI-10 in Salmonella enterica serovar Typhi and located adjacent to tRNAleux. We find that across the Salmonella genus the tRNAleux region is a hypervariable hot spot for horizontal gene transfer; different isolates from the same S. enterica serovar can exhibit significant variation in this region. Many P4 phage, plasmid, and transposable element-associated genes are found adjacent to tRNAleux in both Salmonella and E. coli, suggesting that these mobile genetic elements have played a major role in driving the variability of this region. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
9. Structure, Diversity, and Mobility of the Salmonella Pathogenicity Island 7 Family of Integrative and Conjugative Elements within Enterobacteriaceae.
- Author
-
Seth-Smith, Helena M. B., Fookes, Maria C., Okoro, Chinyere K., Baker, Stephen, Harris, Simon R., Scott, Paul, Pickard, Derek, Quail, Michael A., Churcher, Carol, Sanders, Mandy, Harmse, Johan, Dougan, Gordon, Parkhill, Julian, and Thomson, Nicholas R.
- Subjects
- *
ENTEROBACTERIACEAE , *SALMONELLA , *BACTERIA , *CITROBACTER , *ESCHERICHIA coli - Abstract
Integrative and conjugative elements (ICEs) are self-mobile genetic elements found in the genomes of some bacteria. These elements may confer a fitness advantage upon their host bacteria through the cargo genes that they carry. Salmonella pathogenicity island 7 (SPI-7), found within some pathogenic strains of Salmonella enterica, possesses features indicative of an ICE and carries genes implicated in virulence. We aimed to identify and fully analyze ICEs related to SPI-7 within the genus Salmonella and other Enterobacteriaceae. We report the sequence of two novel SPI-7-1ike elements, found within strains of Salmonella bongori, which share 97% nucleotide identity over conserved regions with SPI-7 and with each other. Although SPI-7 within Salmonella enterica serovar Typhi appears to be fixed within the chromosome, we present evidence that these novel elements are capable of excision and self-mobility. Phylogenetic analyses show that these Salmonella mobile elements share an ancestor which existed approximately 3.6 to 15.8 million years ago. Additionally, we identified more distantly related ICEs, with distinct cargo regions, within other strains of Salmonella as well as within Citrobacter, Erwinia, Escherichia, Photorhabdus, and Yersinia species. In total, we report on a collection of 17 SPI-7 related ICEs within enterobacterial species, of which six are novel. Using comparative and mutational studies, we have denned a core of 27 genes essential for conjugation. We present a growing family of SPl-7-related ICEs whose mobility, abundance, and cargo variability indicate that these elements may have had a large impact on the evolution of the Enterobacteriaceae. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
10. Genomic and Genetic Analysis of Bordetella Bacteriophages Encoding Reverse Transcriptase-Mediated Tropism-Switching Cassettes.
- Author
-
Minghsun Liu, F. Marion, Gingery, Mari, Doulatov, Sergei R., Yichin Liu, Hodes, Asher, Baker, Stephen, Davis, Paul, Simmonds, Mark, Churcher, Carol, Mungall, Karen, Quail, Michael A., Preston, Andrew, Harvill, Eric T., Maskell, Duncan J., Eiserling, Frederick A., Parkhill, Julian, and Miller, Jeff F.
- Subjects
- *
BORDETELLA , *BACTERIOPHAGES , *GENOMES , *NUCLEOTIDE sequence , *GENES , *BACTERIA - Abstract
Liu et al. recently described a group of related temperate bacteriophages that infect Bordetella subspecies and undergo a unique template-dependent, reverse transcriptase-mediated tropism switching phenomenon (Liu et al., Science 295:2091-2094, 2002). Tropism switching results from the introduction of single nucleotide substitutions at defined locations in the VR1 (variable region 1) segment of the mtd (major tropism determinant) gene, which determines specificity for receptors on host bacteria. In this report, we describe the complete nucleotide sequences of the 42.5- to 42.7-kb double-stranded DNA genomes of three related phage isolates and characterize two additional regions of variability. Forty-nine coding sequences were identified. Of these coding sequences, bbp36 contained VR2 (variable region 2), which is highly dynamic and consists of a variable number of identical 19-bp repeats separated by one of three 5-bp spacers, and bpm encodes a DNA adenine methylase with unusual site specificity and a homopolymer tract that functions as a hotspot for frameshift mutations. Morphological and sequence analysis suggests that these Bordetella phage are genetic hybrids of P22 and T7 family genomes, lending further support to the idea that regions encoding protein domains, single genes, or blocks of genes are readily exchanged between bacterial and phage genomes. Bordetella bacteriophages are capable of transducing genetic markers in vitro, and by using animal models, we demonstrated that lysogenic conversion can take place in the mouse respiratory tract during infection. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.