1. In Vivo Splicing and Functional Characterization of Mycobacterium leprae RecA
- Author
-
Klaus Frischkorn, M. Joseph Colston, B Springer, Elaine O. Davis, Erik C. Böttger, and Peter Sander
- Subjects
Recombination, Genetic ,biology ,Mycobacterium smegmatis ,Genetics and Molecular Biology ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,medicine.disease_cause ,Microbiology ,Mycobacterium leprae ,Mycobacterium tuberculosis ,Rec A Recombinases ,In vivo ,Protein splicing ,RNA splicing ,medicine ,Protein Splicing ,bacteria ,Homologous recombination ,Molecular Biology ,Escherichia coli - Abstract
The RecA proteins from Mycobacterium tuberculosis and Mycobacterium leprae contain inteins. In contrast to the M. tuberculosis RecA, the M. leprae RecA is not spliced in Escherichia coli . We demonstrate here that M. leprae RecA is functionally spliced in Mycobacterium smegmatis and produces resistance toward DNA-damaging agents and homologous recombination.
- Published
- 2000
- Full Text
- View/download PDF