8 results on '"Baker, Stephen"'
Search Results
2. Analysis of the Hypervariable Region of the Salmonella enterica Genome Associated with tRNAleuX.
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Bishop, Anne L., Baker, Stephen, Jenks, Sara, Fookes, Maria, Gaora, Peadar Ó., Pickard, Derek, Anjum, Muna, Farrar, Jeremy, Hien, Tran T., Ivens, Al, and Dougan, Gordon
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SALMONELLA , *ESCHERICHIA coli , *ENTEROBACTERIACEAE , *BACTERIAL genetics , *BACTERIOLOGY , *BACTERIAL genomes - Abstract
The divergence of Salmonella enterica and Escherichia coli is estimated to have occurred approximately 140 million years ago. Despite this evolutionary distance, the genomes of these two species still share extensive synteny and homology. However, there are significant differences between the two species in terms of genes putatively acquired via various horizontal transfer events. Here we report on the composition and distribution across the Salmonella genus of a chromosomal region designated SPI-10 in Salmonella enterica serovar Typhi and located adjacent to tRNAleux. We find that across the Salmonella genus the tRNAleux region is a hypervariable hot spot for horizontal gene transfer; different isolates from the same S. enterica serovar can exhibit significant variation in this region. Many P4 phage, plasmid, and transposable element-associated genes are found adjacent to tRNAleux in both Salmonella and E. coli, suggesting that these mobile genetic elements have played a major role in driving the variability of this region. [ABSTRACT FROM AUTHOR]
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- 2005
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3. Genomic Comparison of Salmonella enterica Serovars and Salmonella bongori by Use of an S. enterica Serovar Typhimurium DNA Microarray.
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Chan, Kaman, Baker, Stephen, Kim, Charles C., Detweiler, Corrella S., Dougan, Gordon, and Falkow, Stanley
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SALMONELLA enteritidis , *DNA microarrays - Abstract
The genus Salmonella consists of over 2,200 serovars that differ in their host range and ability to cause disease despite their close genetic relatedness. The genetic factors that influence each serovar's level of host adaptation, how they evolved or were acquired, their influence on the evolution of each serovar, and the phylogenic relationships between the serovars are of great interest as they provide insight into the mechanisms behind these differences in host range and disease progression. We have used an Salmonella enterica serovar Typhimurium spotted DNA microarray to perform genomic hybridizations of various serovars and strains of both S. enterica (subspecies I and IIIa) and Salmonella bongori to gain insight into the genetic organization of the serovars. Our results are generally consistent with previously published DNA association and multilocus enzyme electrophoresis data. Our findings also reveal novel information. We observe a more distant relationship of serovar Arizona (subspecies IIIa) from the subspecies I serovars than previously measured. We also observe variability in the Arizona SPI-2 pathogenicity island, indicating that it has evolved in a manner distinct from the other serovars. In addition, we identify shared genetic features of S. enterica serovars Typhi, Paratyphi A, and Sendal that parallel their unique ability to cause enteric fever in humans. Therefore, whereas the taxonomic organization of Salmonella into serogroups provides a good first approximation of genetic relatedness, we show that it does not account for genomic changes that contribute to a serovar's degree of host adaptation. [ABSTRACT FROM AUTHOR]
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- 2003
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4. Composition, Acquisition, and Distribution of the Vi Exopolysaccharide-Encoding Salmonella enterica Pathogenicity Island SPI-7.
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Pickard, Derek, Wain, John, Baker, Stephen, Line, Alexandra, Chohan, Sonia, Fookes, Maria, Barron, Andrew, Gaora, Peadar Ó., Chabalgoity, José A., Thanky, Niren, Scholes, Christoph, Thomson, Nicholas, Quail, Michael, Parkhill, Julian, and Dougan, Gordon
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SALMONELLA , *MICROBIAL exopolysaccharides , *BACTERIOLOGY - Abstract
Vi capsular polysaccharide production is encoded by the viaB locus, which has a limited distribution in Salmonella enterica serovars. In S. enterica serovar Typhi, viaB is encoded on a 134-kb pathogenicity island known as SPI-7 that is located between partially duplicated tRNA[sup pheU] sites. Functional and bioinformatic analysis suggests that SPI-7 has a mosaic structure and may have evolved as a consequence of several independent insertion events. Analysis of viaB-associated DNA in Vi-positive S. enterica serovar Paratyphi C and S. enterica serovar Dublin isolates revealed the presence of similar SPI-7 islands. In S. enterica serovars Paratyphi C and Dublin, the SopE bacteriophage and a 15-kb fragment adjacent to the intact tRNA[sup pheU] site were absent. In S. enterica serovar Paratyphi C only, a region encoding a type IV pilus involved in the adherence or S. enterica serovar Typhi to host cells was missing. The remainder of the SPI-7 islands investigated exhibited over 99% DNA sequence identity in the three serovars. Of 30 other Salmonella serovars examined, 24 contained no insertions at the equivalent tRNA[sup pheU] site, 2 had a 3.7-kb insertion, and 4 showed sequence variation at the tRNA[sup pheU]-phoN junction, which was not analyzed further. Sequence analysis of the SPI-7 region from S. enterica serovar Typhi strain CT18 revealed significant synteny with clusters of genes from a variety of saprophytic bacteria and phytobacteria, including Pseudomonas aeruginosa and Xanthomonas axonopodis pv. citri. This analysis suggested that SPI-7 may be a mobile element, such as a conjugative transposon or an integrated plasmid remnant. [ABSTRACT FROM AUTHOR]
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- 2003
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5. Structure, Diversity, and Mobility of the Salmonella Pathogenicity Island 7 Family of Integrative and Conjugative Elements within Enterobacteriaceae.
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Seth-Smith, Helena M. B., Fookes, Maria C., Okoro, Chinyere K., Baker, Stephen, Harris, Simon R., Scott, Paul, Pickard, Derek, Quail, Michael A., Churcher, Carol, Sanders, Mandy, Harmse, Johan, Dougan, Gordon, Parkhill, Julian, and Thomson, Nicholas R.
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ENTEROBACTERIACEAE , *SALMONELLA , *BACTERIA , *CITROBACTER , *ESCHERICHIA coli - Abstract
Integrative and conjugative elements (ICEs) are self-mobile genetic elements found in the genomes of some bacteria. These elements may confer a fitness advantage upon their host bacteria through the cargo genes that they carry. Salmonella pathogenicity island 7 (SPI-7), found within some pathogenic strains of Salmonella enterica, possesses features indicative of an ICE and carries genes implicated in virulence. We aimed to identify and fully analyze ICEs related to SPI-7 within the genus Salmonella and other Enterobacteriaceae. We report the sequence of two novel SPI-7-1ike elements, found within strains of Salmonella bongori, which share 97% nucleotide identity over conserved regions with SPI-7 and with each other. Although SPI-7 within Salmonella enterica serovar Typhi appears to be fixed within the chromosome, we present evidence that these novel elements are capable of excision and self-mobility. Phylogenetic analyses show that these Salmonella mobile elements share an ancestor which existed approximately 3.6 to 15.8 million years ago. Additionally, we identified more distantly related ICEs, with distinct cargo regions, within other strains of Salmonella as well as within Citrobacter, Erwinia, Escherichia, Photorhabdus, and Yersinia species. In total, we report on a collection of 17 SPI-7 related ICEs within enterobacterial species, of which six are novel. Using comparative and mutational studies, we have denned a core of 27 genes essential for conjugation. We present a growing family of SPl-7-related ICEs whose mobility, abundance, and cargo variability indicate that these elements may have had a large impact on the evolution of the Enterobacteriaceae. [ABSTRACT FROM AUTHOR]
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- 2012
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6. Genomic and Genetic Analysis of Bordetella Bacteriophages Encoding Reverse Transcriptase-Mediated Tropism-Switching Cassettes.
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Minghsun Liu, F. Marion, Gingery, Mari, Doulatov, Sergei R., Yichin Liu, Hodes, Asher, Baker, Stephen, Davis, Paul, Simmonds, Mark, Churcher, Carol, Mungall, Karen, Quail, Michael A., Preston, Andrew, Harvill, Eric T., Maskell, Duncan J., Eiserling, Frederick A., Parkhill, Julian, and Miller, Jeff F.
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BORDETELLA , *BACTERIOPHAGES , *GENOMES , *NUCLEOTIDE sequence , *GENES , *BACTERIA - Abstract
Liu et al. recently described a group of related temperate bacteriophages that infect Bordetella subspecies and undergo a unique template-dependent, reverse transcriptase-mediated tropism switching phenomenon (Liu et al., Science 295:2091-2094, 2002). Tropism switching results from the introduction of single nucleotide substitutions at defined locations in the VR1 (variable region 1) segment of the mtd (major tropism determinant) gene, which determines specificity for receptors on host bacteria. In this report, we describe the complete nucleotide sequences of the 42.5- to 42.7-kb double-stranded DNA genomes of three related phage isolates and characterize two additional regions of variability. Forty-nine coding sequences were identified. Of these coding sequences, bbp36 contained VR2 (variable region 2), which is highly dynamic and consists of a variable number of identical 19-bp repeats separated by one of three 5-bp spacers, and bpm encodes a DNA adenine methylase with unusual site specificity and a homopolymer tract that functions as a hotspot for frameshift mutations. Morphological and sequence analysis suggests that these Bordetella phage are genetic hybrids of P22 and T7 family genomes, lending further support to the idea that regions encoding protein domains, single genes, or blocks of genes are readily exchanged between bacterial and phage genomes. Bordetella bacteriophages are capable of transducing genetic markers in vitro, and by using animal models, we demonstrated that lysogenic conversion can take place in the mouse respiratory tract during infection. [ABSTRACT FROM AUTHOR]
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- 2004
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7. Molecular Characterization of the Salmonella enterica Serovar Typhi Vi-Typing Bacteriophage E1.
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Pickard, Derek, Thomson, Nicholas R., Baker, Stephen, Wain, John, Pardo, Mercedes, Goulding, David, Hamlin, Nancy, Choudhary, Jyoti, Threfall, John, and Dougan, Gordon
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BACTERIOPHAGES , *VIRUSES , *MICROBIAL virulence , *PATHOGENIC microorganisms , *SALMONELLA , *MASS spectrometry - Abstract
Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage using a number of molecular approaches. The absolute requirement for Vi capsule expression for infectivity was demonstrated using different Vi-negative S. enterica derivatives. The phage particles were shown to have an icosahedral head and a long noncontractile tail structure. The genome is 45,362 bp in length with defined capsid and tail regions that exhibit significant homology to the S. enterica transducing phage ES18. Mass spectrometry was used to confirm the presence of a number of hypothetical proteins in the Vi phage E1 particle and demonstrate that a number of phage proteins are modified posttranslationally. The genome of the Vi phage E1 is significantly related to other bacteriophages belonging to the same serovar Typhi phage-typing set, and we demonstrate a role for phage DNA modification in determining host specificity. [ABSTRACT FROM AUTHOR]
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- 2008
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8. Yersinia pestis pFra Shows Biovar-Specific Differences and Recent Common Ancestry with a...
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Prentice, Michael B., James, Keith D., Parkhill, Julian, Baker, Stephen G., Stevens, Kim, Simmonds, Mark N., Mungall, Karen L., Churcher, Carol, Oyston, Petra C.F., Titball], Richard W., Wren, Brendan W., Wain, John, Pickard, Derek, Tran Tinh Hien, Farrar, Jeremy J., and Dougan, Gordon
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YERSINIA pestis , *PLASMIDS - Abstract
Demonstrates specific plasmid rearrangements in different Yersinia pestis strains which distinguish Yersinia pestis biovar Orientalis strains from other biovars. Factors influencing the rise and fall of plague epidemics; Key difference between Yersinia pseudotuberculosis and Yersinia pestis; Yersinia pestis interbiovar comparisons.
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- 2001
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